LOCUS GF100473 660 bp DNA linear STS 05-MAY-2009 DEFINITION LMU0501 Bovine sperm and blood Bos taurus STS genomic, sequence tagged site. ACCESSION GF100473 VERSION GF100473.1 KEYWORDS STS. SOURCE Bos taurus (cattle) ORGANISM Bos taurus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Laurasiatheria; Cetartiodactyla; Ruminantia; Pecora; Bovidae; Bovinae; Bos. REFERENCE 1 (bases 1 to 660) AUTHORS Awad,A., Russ,I., Emmerling,R., Forster,M. and Medugorac,I. TITLE Confirmation and Refinement of a QTL on BTA05 affecting Milk Production Traits in the Fleckvieh Dual Purpose Cattle Breed JOURNAL Unpublished (2008) COMMENT Contact: Ashraf Awad Chair of Animal Genetics and Husbandry Faculty of Veterinary Medicine, Ludwig-Maximilians-University Munich Veterinaerstr. 13, 80539 Munich, Germany Tel: ++49-89-21802973 Fax: ++49-89-344925 Email: ashraf.awad@gen.vetmed.uni-muenchen.de Primer A: CATGTTGGTAATGAATGGGCTA Primer B: TAATCAACGGCATCATCAGG STS size: 267 PCR Profile: Initial denaturation: 95 degrees C for 2.00 minute(s) Denaturation: 94 degrees C for 30.00 second(s) Annealing: 59 degrees C for 120.00 second(s) Polymerization: 72 degrees C for 75.00 second(s) PCR Cycles: 29 Final extension: 68 degrees C for 60.00 minute(s) Thermal Cycler: Primus 96plus (MWG-Biotech) and PTC-100 (MJ Research) Protocol: Template: 30ng Primer: each 0, 25 uM dNTPs: each 200 uM Taq Polymerase: 0.2units/ul Total Vol: 15 ul Buffer: MgCl2: 2 mM KCl: 50 mM Tris-HCl: 10 mM pH: 9.0 chromosome 05. FEATURES Location/Qualifiers source 1..660 /organism="Bos taurus" /mol_type="genomic DNA" /db_xref="taxon:9913" /clone_lib="Bovine sperm and blood" /note="; Dinucleotide repeats in genomic regions with low marker density." STS 207..473 primer_bind 207..228 primer_bind complement(454..473) BASE COUNT 208 a 120 c 86 g 246 t ORIGIN 1 tgcagatagt tttttgagat cctgaccact tagactgagt tatcccttca ctaagcatta 61 tgacataatc tgagtatatt ttatcttaat caagtaactt acttattcag ttaataatca 121 tacgatgtaa tactgaacag aactcagacc ttagccctat ctttccattt tatatatgaa 181 aaagcataac attttaattt aaaaatcatg ttggtaatga atgggctata tcttttttaa 241 aaaccttttt tgaggtatgc acttgaaaca ctagatcctt aaaaaactga aaagccaagc 301 ttttaaaaat aaccaccttg caatatatat atatatatat atatatatct gtatataagt 361 gccctacaat taatgttcta taattagtgt tttgcttctc catttttctc ctttaacaat 421 ctcttccttc tactcatcac ttttgcttca aatcctgatg atgccgttga ttagatgtga 481 gttattgctt taggtaagtg gtacaagtgg tgtttataaa ctttccttgg gtatcacaat 541 ttttctacct cttatcctct ctatatcagg aattctaaga ttgtctcttt attcaactaa 601 tccatcactg aaaagtttta ggaggtccca tctcacaaaa agacagaaca gcttggctga // LOCUS GF100474 660 bp DNA linear STS 05-MAY-2009 DEFINITION LMU0502 Bovine sperm and blood Bos taurus STS genomic, sequence tagged site. ACCESSION GF100474 VERSION GF100474.1 KEYWORDS STS. SOURCE Bos taurus (cattle) ORGANISM Bos taurus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Laurasiatheria; Cetartiodactyla; Ruminantia; Pecora; Bovidae; Bovinae; Bos. REFERENCE 1 (bases 1 to 660) AUTHORS Awad,A., Russ,I., Emmerling,R., Forster,M. and Medugorac,I. TITLE Confirmation and Refinement of a QTL on BTA05 affecting Milk Production Traits in the Fleckvieh Dual Purpose Cattle Breed JOURNAL Unpublished (2008) COMMENT Contact: Ashraf Awad Chair of Animal Genetics and Husbandry Faculty of Veterinary Medicine, Ludwig-Maximilians-University Munich Veterinaerstr. 13, 80539 Munich, Germany Tel: ++49-89-21802973 Fax: ++49-89-344925 Email: ashraf.awad@gen.vetmed.uni-muenchen.de Primer A: TGGAAGAATATGCAGGTAACTCT Primer B: GTCGCTCTTTGTGGCTTCAC STS size: 300 PCR Profile: Initial denaturation: 95 degrees C for 2.00 minute(s) Denaturation: 94 degrees C for 30.00 second(s) Annealing: 59 degrees C for 120.00 second(s) Polymerization: 72 degrees C for 75.00 second(s) PCR Cycles: 29 Final extension: 68 degrees C for 60.00 minute(s) Thermal Cycler: Primus 96plus (MWG-Biotech) and PTC-100 (MJ Research) Protocol: Template: 30ng Primer: each 0, 25 uM dNTPs: each 200 uM Taq Polymerase: 0.2units/ul Total Vol: 15 ul Buffer: MgCl2: 2 mM KCl: 50 mM Tris-HCl: 10 mM pH: 9.0 chromosome 05. FEATURES Location/Qualifiers source 1..660 /organism="Bos taurus" /mol_type="genomic DNA" /db_xref="taxon:9913" /clone_lib="Bovine sperm and blood" /note="; Dinucleotide repeats in genomic regions with low marker density." STS 285..584 primer_bind 285..307 primer_bind complement(565..584) BASE COUNT 229 a 80 c 111 g 240 t ORIGIN 1 cctacttgaa tctatgcatc tttgtgatgg gctcttccca gtaatgaaac caagtaaacc 61 aaatattgaa cccagacctt ggtagcataa agtgataagc caagacctta aaatatagtg 121 tagaatgtta ttaatataat tgtcaccaag tagtgttagt aactgtgttt agaaagagca 181 aaaaatgcat tttagttaat atatgtgata gaattaaaat taattttaaa tatttatttt 241 attctagtat atcttttatg tttttataat ataacatatt tatatggaag aatatgcagg 301 taactcttaa atgaatatat atatatatat atatatatat atctgaaagc agctttacaa 361 ttttctctat gtgagaaatg aatggtgaaa aattttgaga cctgtggttt taatacttgc 421 ctataggtcc tgagtgttgg agttgggtct aaagttcaac gtaatagttc aaagtgacac 481 tcttatcttg agctaatggc aatattgaaa attggaaagt gcctttagga attttctttc 541 aggtgttgtt tctgaagaat aattgtgaag ccacaaagag cgactttctg tcatccttgt 601 tttgttctct ttaattaaaa aaataaagag gaagtaattt actactgaaa taatttctgc //