| 1 |
GSM48951 |
WTCy3/Rv2869c Cy5 no Tween #1 |
10,080 |
Sloan Kettering Institute for Cancer Research |
2005-04-23 |
[Oligo Array] M. tuberculosis oligo (GPL1983) |
RNA |
Mycobacterium tuberculosis
 |
unclassified |
source_name:Rv2869c mutant Wild Type M. tuberculosis title:WTCy3/Rv2869c Cy5 no Tween #1 description:o All RNA was from the indicated strain of M. tuberculosis (either wild type M. tuberculosis Erdman or the Mtb Rv2869c null mutant •RNA was isolated from logarithmic phase cultures at OD600 .3. For (+Tween) conditions, the media was Middlebrook 7H9 with 10% OADC supplement, .5% glycerol, and .05% Tween 80. For (-Tween) conditions, bacilli were transferred to identical media without Tween 80 and harvested for RNA preparation 18hours later. Protocol for preparing the hybridization extract: 15ml of liquid culture described above were collected by centrifugation at 3000g, and cell pellets frozen on dry ice. Frozen pellets were resuspended in 1ml of Trizol reagent (Invitrogen) and lysed by mechanical disruption using .7mm Zirconia beads for 3 x 1min in a Mini bead beater 8 (Biospec products) at maximum speed. The remainder of the procedure was according to the Trizol manufacturers instructions with the addition of the optional high salt precipitation step. RNA was subsequently cleaned using Qiagen RNAeasy columns according ot the manufacturers RNA cleanup protocol. Labeling protocol(s). 5-7.5micrograms of total RNA was labeled using the Fairplay microrray labeling kit (Stratagene) with substitution of Random Hexamer primers. Aminoallyl labeled CDNA was coupled to Cy3/Cy5 using Post labeling reactive dye packs (Amersham). Excess dye was removed using Qiaquick nucleotide removal columns according to the manufacturer’s instructions. |
| 2 |
GSM49626 |
1_GLC_FRU |
5,736 |
North Carolina State University |
2005-04-26 |
[cDNA Array] Whole Genome Array L. acidophilus NCFM (GPL1401) |
RNA |
Lactobacillus acidophilus
 |
unclassified |
source_name:L. acidophilus, glucose L. acidophilus, fructose title:1_GLC_FRU description:L. acidophilus cells, transferred at least five times in modified MRS medium (Barrangou et al, 2003) containing the carbohydrate of interest, were grown to an OD600 of 0.6, harvested and processed for microarray experiments (Azcarate-Peril et al, 2005). |
| 3 |
GSM49627 |
2_SUC_GLC |
5,736 |
North Carolina State University |
2005-04-26 |
[cDNA Array] Whole Genome Array L. acidophilus NCFM (GPL1401) |
RNA |
Lactobacillus acidophilus
 |
unclassified |
source_name:L. acidophilus, sucrose L. acidophilus, glucose title:2_SUC_GLC description:L. acidophilus cells, transferred at least five times in modified MRS medium (Barrangou et al, 2003) containing the carbohydrate of interest, were grown to an OD600 of 0.6, harvested and processed for microarray experiments (Azcarate-Peril et al, 2005). |
| 4 |
GSM49628 |
3_GLC_FOS |
5,736 |
North Carolina State University |
2005-04-26 |
[cDNA Array] Whole Genome Array L. acidophilus NCFM (GPL1401) |
RNA |
Lactobacillus acidophilus
 |
unclassified |
source_name:L. acidophilus, glucose L. acidophilus, fructooligosaccharide title:3_GLC_FOS description:L. acidophilus cells, transferred at least five times in modified MRS medium (Barrangou et al, 2003) containing the carbohydrate of interest, were grown to an OD600 of 0.6, harvested and processed for microarray experiments (Azcarate-Peril et al, 2005). |
| 5 |
GSM49629 |
4_RAF_GLC |
5,736 |
North Carolina State University |
2005-04-26 |
[cDNA Array] Whole Genome Array L. acidophilus NCFM (GPL1401) |
RNA |
Lactobacillus acidophilus
 |
unclassified |
source_name:L. acidophilus, raffinose L. acidophilus, glucose title:4_RAF_GLC description:L. acidophilus cells, transferred at least five times in modified MRS medium (Barrangou et al, 2003) containing the carbohydrate of interest, were grown to an OD600 of 0.6, harvested and processed for microarray experiments (Azcarate-Peril et al, 2005). |
| 6 |
GSM49630 |
5_GLC_LAC |
5,736 |
North Carolina State University |
2005-04-26 |
[cDNA Array] Whole Genome Array L. acidophilus NCFM (GPL1401) |
RNA |
Lactobacillus acidophilus
 |
unclassified |
source_name:L. acidophilus, glucose L. acidophilus, lactose title:5_GLC_LAC description:L. acidophilus cells, transferred at least five times in modified MRS medium (Barrangou et al, 2003) containing the carbohydrate of interest, were grown to an OD600 of 0.6, harvested and processed for microarray experiments (Azcarate-Peril et al, 2005). |
| 7 |
GSM49631 |
6_GAL_GLC |
5,736 |
North Carolina State University |
2005-04-26 |
[cDNA Array] Whole Genome Array L. acidophilus NCFM (GPL1401) |
RNA |
Lactobacillus acidophilus
 |
unclassified |
source_name:L. acidophilus, galactose L. acidophilus, glucose title:6_GAL_GLC description:L. acidophilus cells, transferred at least five times in modified MRS medium (Barrangou et al, 2003) containing the carbohydrate of interest, were grown to an OD600 of 0.6, harvested and processed for microarray experiments (Azcarate-Peril et al, 2005). |
| 8 |
GSM49632 |
7_GLC_TRE |
5,736 |
North Carolina State University |
2005-04-26 |
[cDNA Array] Whole Genome Array L. acidophilus NCFM (GPL1401) |
RNA |
Lactobacillus acidophilus
 |
unclassified |
source_name:L. acidophilus, glucose L. acidophilus, trehalose title:7_GLC_TRE description:L. acidophilus cells, transferred at least five times in modified MRS medium (Barrangou et al, 2003) containing the carbohydrate of interest, were grown to an OD600 of 0.6, harvested and processed for microarray experiments (Azcarate-Peril et al, 2005). |
| 9 |
GSM49633 |
8_FRU_SUC |
5,736 |
North Carolina State University |
2005-04-26 |
[cDNA Array] Whole Genome Array L. acidophilus NCFM (GPL1401) |
RNA |
Lactobacillus acidophilus
 |
unclassified |
source_name:L. acidophilus, fructose L. acidophilus, sucrose title:8_FRU_SUC description:L. acidophilus cells, transferred at least five times in modified MRS medium (Barrangou et al, 2003) containing the carbohydrate of interest, were grown to an OD600 of 0.6, harvested and processed for microarray experiments (Azcarate-Peril et al, 2005). |
| 10 |
GSM49634 |
9_FOS_FRU |
5,736 |
North Carolina State University |
2005-04-26 |
[cDNA Array] Whole Genome Array L. acidophilus NCFM (GPL1401) |
RNA |
Lactobacillus acidophilus
 |
unclassified |
source_name:L. acidophilus, fructooligosaccharides L. acidophilus, fructose title:9_FOS_FRU description:L. acidophilus cells, transferred at least five times in modified MRS medium (Barrangou et al, 2003) containing the carbohydrate of interest, were grown to an OD600 of 0.6, harvested and processed for microarray experiments (Azcarate-Peril et al, 2005). |
| 11 |
GSM49635 |
10_FRU_RAF |
5,736 |
North Carolina State University |
2005-04-26 |
[cDNA Array] Whole Genome Array L. acidophilus NCFM (GPL1401) |
RNA |
Lactobacillus acidophilus
 |
unclassified |
source_name:L. acidophilus, fructose L. acidophilus, raffinose title:10_FRU_RAF description:L. acidophilus cells, transferred at least five times in modified MRS medium (Barrangou et al, 2003) containing the carbohydrate of interest, were grown to an OD600 of 0.6, harvested and processed for microarray experiments (Azcarate-Peril et al, 2005). |
| 12 |
GSM49636 |
11_LAC_FRU |
5,736 |
North Carolina State University |
2005-04-26 |
[cDNA Array] Whole Genome Array L. acidophilus NCFM (GPL1401) |
RNA |
Lactobacillus acidophilus
 |
unclassified |
source_name:L. acidophilus, lactose L. acidophilus, fructose title:11_LAC_FRU description:L. acidophilus cells, transferred at least five times in modified MRS medium (Barrangou et al, 2003) containing the carbohydrate of interest, were grown to an OD600 of 0.6, harvested and processed for microarray experiments (Azcarate-Peril et al, 2005). |
| 13 |
GSM49637 |
12_FRU_GAL |
5,736 |
North Carolina State University |
2005-04-26 |
[cDNA Array] Whole Genome Array L. acidophilus NCFM (GPL1401) |
RNA |
Lactobacillus acidophilus
 |
unclassified |
source_name:L. acidophilus, fructose L. acidophilus, galactose title:12_FRU_GAL description:L. acidophilus cells, transferred at least five times in modified MRS medium (Barrangou et al, 2003) containing the carbohydrate of interest, were grown to an OD600 of 0.6, harvested and processed for microarray experiments (Azcarate-Peril et al, 2005). |
| 14 |
GSM49638 |
13_TRE_FRU |
5,736 |
North Carolina State University |
2005-04-26 |
[cDNA Array] Whole Genome Array L. acidophilus NCFM (GPL1401) |
RNA |
Lactobacillus acidophilus
 |
unclassified |
source_name:L. acidophilus, trehalose L. acidophilus, fructose title:13_TRE_FRU description:L. acidophilus cells, transferred at least five times in modified MRS medium (Barrangou et al, 2003) containing the carbohydrate of interest, were grown to an OD600 of 0.6, harvested and processed for microarray experiments (Azcarate-Peril et al, 2005). |
| 15 |
GSM49639 |
14_SUC_FOS |
5,736 |
North Carolina State University |
2005-04-26 |
[cDNA Array] Whole Genome Array L. acidophilus NCFM (GPL1401) |
RNA |
Lactobacillus acidophilus
 |
unclassified |
source_name:L. acidophilus, sucrose L. acidophilus, fructooligosaccharides title:14_SUC_FOS description:L. acidophilus cells, transferred at least five times in modified MRS medium (Barrangou et al, 2003) containing the carbohydrate of interest, were grown to an OD600 of 0.6, harvested and processed for microarray experiments (Azcarate-Peril et al, 2005). |
| 16 |
GSM49640 |
15_RAF_SUC |
5,736 |
North Carolina State University |
2005-04-26 |
[cDNA Array] Whole Genome Array L. acidophilus NCFM (GPL1401) |
RNA |
Lactobacillus acidophilus
 |
unclassified |
source_name:L. acidophilus, raffinose L. acidophilus, sucrose title:15_RAF_SUC description:L. acidophilus cells, transferred at least five times in modified MRS medium (Barrangou et al, 2003) containing the carbohydrate of interest, were grown to an OD600 of 0.6, harvested and processed for microarray experiments (Azcarate-Peril et al, 2005). |
| 17 |
GSM49641 |
16_SUC_LAC |
5,736 |
North Carolina State University |
2005-04-26 |
[cDNA Array] Whole Genome Array L. acidophilus NCFM (GPL1401) |
RNA |
Lactobacillus acidophilus
 |
unclassified |
source_name:L. acidophilus, sucrose L. acidophilus, lactose title:16_SUC_LAC description:L. acidophilus cells, transferred at least five times in modified MRS medium (Barrangou et al, 2003) containing the carbohydrate of interest, were grown to an OD600 of 0.6, harvested and processed for microarray experiments (Azcarate-Peril et al, 2005). |
| 18 |
GSM49642 |
17_GAL_SUC |
5,736 |
North Carolina State University |
2005-04-26 |
[cDNA Array] Whole Genome Array L. acidophilus NCFM (GPL1401) |
RNA |
Lactobacillus acidophilus
 |
unclassified |
source_name:L. acidophilus, galactose L. acidophilus, sucrose title:17_GAL_SUC description:L. acidophilus cells, transferred at least five times in modified MRS medium (Barrangou et al, 2003) containing the carbohydrate of interest, were grown to an OD600 of 0.6, harvested and processed for microarray experiments (Azcarate-Peril et al, 2005). |
| 19 |
GSM49643 |
18_SUC_TRE |
5,736 |
North Carolina State University |
2005-04-26 |
[cDNA Array] Whole Genome Array L. acidophilus NCFM (GPL1401) |
RNA |
Lactobacillus acidophilus
 |
unclassified |
source_name:L. acidophilus, sucrose L. acidophilus, trehalose title:18_SUC_TRE description:L. acidophilus cells, transferred at least five times in modified MRS medium (Barrangou et al, 2003) containing the carbohydrate of interest, were grown to an OD600 of 0.6, harvested and processed for microarray experiments (Azcarate-Peril et al, 2005). |
| 20 |
GSM49644 |
19_FOS_RAF |
5,736 |
North Carolina State University |
2005-04-26 |
[cDNA Array] Whole Genome Array L. acidophilus NCFM (GPL1401) |
RNA |
Lactobacillus acidophilus
 |
unclassified |
source_name:L. acidophilus, fructooligosaccharides L. acidophilus, raffinose title:19_FOS_RAF description:L. acidophilus cells, transferred at least five times in modified MRS medium (Barrangou et al, 2003) containing the carbohydrate of interest, were grown to an OD600 of 0.6, harvested and processed for microarray experiments (Azcarate-Peril et al, 2005). |