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MPSS, Massively Parallel Signature Sequencing - MPSS (classic method), Sequencing from the 3' most DpnII site from mRNA using Lynx's MPSS technology. MPSS allows a comprehensive survey of all expressed transcripts in a cell or tissue, without apriori knowledge. This dataset was annotated using a combination of LocusLink, Unigene (build 171) and the latest human build as provided from UCSC (hg16). Annotation Classe Table: Virtual Signature Class mRNA Orientation Poly-Adenylation Features Position 0 Either - Repeat Warning Not applicable Not applicable 1 Forward Strand Poly-A Signal, Poly-A Tail 3' most 2 Forward Strand Poly-A Signal 3' most 3 Forward Strand Poly-A Tail 3' most 4 Forward Strand None 3' most 5 Forward Strand None Not 3' most 6 Forward Strand Internal Poly-A Not 3' most 11 Reverse Strand Poly-A Signal, Poly-A Tail 5' most 12 Reverse Strand Poly-A Signal 5' most 13 Reverse Strand Poly-A Tail 5' most 14 Reverse Strand None 5' most 15 Reverse Strand None Not 5' most 16 Reverse Strand Internal Poly-A Not 5' most 22 Unknown Poly-A Signal Last before signal 23 Unknown Poly-A Tail Last before tail 24 Unknown None Last in sequence 25 Unknown None Not last 26 Unknown Internal Poly-A Not 3' most 1000 Unknown- Derived from Genomic Sequence Not applicable Not applicable Keywords = MPSS, mRNA sequencing, tags
MPSS tags from techical comparison of SAGE and MPSS
30,099
University of Oxford
2007-07-27
Homo sapiens
MPSS
MPSS, MPSS tags from techical comparison of SAGE and MPSS, A list of all tags obtained in a set of MPSS libraries used to analyse the transcriptome of a CD4+ T cell clone.