| 1 |
GSM60839 |
SAGE_Drosophila pseudoobscura_Male |
7,672 |
Veterinärmedizinische Universität Wien |
2005-06-13 |
[SAGE NlaIII] SAGE:10:NlaIII:Drosophila pseudoobscura (GPL2537) |
SAGE |
Drosophila pseudoobscura
 |
unclassified |
source_name:male flies title:SAGE_Drosophila pseudoobscura_Male description:The mRNA was isolated from total RNA using a biotin-labeled Tn oligo and Dynal beads. Double stranded cDNA was generated with Superscript II reverse transcriptase and DNA polymerase I. The resulting cDNA library was digested with NlaIII (anchoring enzyme). 3’ restriction fragments were recovered with Dynal beads and split into two populations. Each aliquot was ligated to one of the two annealed linker pairs. After extensive washing tags were created by digestion with BsmFI (tagging enzyme). Tags were blunted with T4 polymerase and ligated to generate ditags. Ditags were PCR amplified and digested with NlaIII. Restriction fragments were separated on a 12% polyacrylamide gel and the band containing the ditags was isolated from the gel. The purified inserts were concatemerized and ligated into pZero and transformed into EDH5? electromax cells. |
| 2 |
GSM60840 |
SAGE_Drosophila pseudoobscura_Female |
7,486 |
Veterinärmedizinische Universität Wien |
2005-06-13 |
[SAGE NlaIII] SAGE:10:NlaIII:Drosophila pseudoobscura (GPL2537) |
SAGE |
Drosophila pseudoobscura
 |
unclassified |
source_name:female flies title:SAGE_Drosophila pseudoobscura_Female description:The mRNA was isolated from total RNA using a biotin-labeled Tn oligo and Dynal beads. Double stranded cDNA was generated with Superscript II reverse transcriptase and DNA polymerase I. The resulting cDNA library was digested with NlaIII (anchoring enzyme). 3’ restriction fragments were recovered with Dynal beads and split into two populations. Each aliquot was ligated to one of the two annealed linker pairs. After extensive washing tags were created by digestion with BsmFI (tagging enzyme). Tags were blunted with T4 polymerase and ligated to generate ditags. Ditags were PCR amplified and digested with NlaIII. Restriction fragments were separated on a 12% polyacrylamide gel and the band containing the ditags was isolated from the gel. The purified inserts were concatemerized and ligated into pZero and transformed into EDH5? electromax cells. |
| 3 |
GSM255664 |
Munoz_OP_untreated |
7,806 |
USDA |
2008-01-08 |
[SAGE NlaIII] SAGE:10:NlaIII:Rhipicephalus microplus (GPL6351) |
SAGE |
Rhipicephalus microplus
 |
unclassified |
source_name:Munoz stratin_f13 generation_12-16d old larvae title:Munoz_OP_untreated description:For more details see: Insect Molecular Biology (2007) 16(1), 49-60. |
| 4 |
GSM255665 |
San Roman_OP_treated |
7,714 |
USDA |
2008-01-08 |
[SAGE NlaIII] SAGE:10:NlaIII:Rhipicephalus microplus (GPL6351) |
SAGE |
Rhipicephalus microplus
 |
unclassified |
source_name:San Roman_f18 generation_12-16 days old larvae title:San Roman_OP_treated description:For more details see: Insect Molecular Biology (2007) 16(1), 49-60. |
| 5 |
GSM255666 |
San Roman_OP_untreated |
6,095 |
USDA |
2008-01-08 |
[SAGE NlaIII] SAGE:10:NlaIII:Rhipicephalus microplus (GPL6351) |
SAGE |
Rhipicephalus microplus
 |
unclassified |
source_name:San Roman_f18 generation_12-16 days old larvae title:San Roman_OP_untreated description:For more details see: Insect Molecular Biology (2007) 16(1), 49-60. |
| 6 |
GSM255662 |
Munoz_OP_treated |
6,030 |
USDA |
2008-01-08 |
[SAGE NlaIII] SAGE:10:NlaIII:Rhipicephalus microplus (GPL6351) |
SAGE |
Rhipicephalus microplus
 |
unclassified |
source_name:Munoz stratin_f13 generation_12-16d old larvae title:Munoz_OP_treated description:For more details see: Insect Molecular Biology (2007) 16(1), 49-60. |
| 7 |
GSM249492 |
Ovary Babesia Infected (6 hours) B. microplus |
5,315 |
USDA |
2007-12-13 |
[SAGE NlaIII] SAGE:17:NlaIII:Rhipicephalus microplus (GPL6274) |
SAGE |
Rhipicephalus microplus
 |
ovary |
B. microplus Adult ovary tissue |
| 8 |
GSM249490 |
Ovary control(time zero) B. microplus |
3,933 |
USDA |
2007-12-13 |
[SAGE NlaIII] SAGE:17:NlaIII:Rhipicephalus microplus (GPL6274) |
SAGE |
Rhipicephalus microplus
 |
ovary |
Adult Ovary [B. microplus] |
| 9 |
GSM109148 |
Grass Shrimp Endosulfan-exposed Library |
5,286 |
University of Southern Mississippi |
2006-05-16 |
[SAGE NlaIII] SAGE:17:NlaIII:Palaemonetes pugio (GPL3770) |
SAGE |
Palaemonetes pugio
 |
unclassified |
source_name:Grass shrimp Endosulfan-exposed title:Grass Shrimp Endosulfan-exposed Library description:This library was constructed from Endosulfan-exposed shrimp in an exposure to identify differentially expressed genes in shrimp following exposure to fipronil, endosulfan, or cadmium. |
| 10 |
GSM109146 |
Grass Shrimp Control Library |
4,814 |
University of Southern Mississippi |
2006-05-16 |
[SAGE NlaIII] SAGE:17:NlaIII:Palaemonetes pugio (GPL3770) |
SAGE |
Palaemonetes pugio
 |
unclassified |
source_name:Grass shrimp control title:Grass Shrimp Control Library description:This library was constructed from control shrimp in an exposure to identify differentially expressed genes in shrimp following exposure to fipronil, endosulfan, or cadmium. |
| 11 |
GSM109150 |
Grass Shrimp Cadmium-exposed Library |
4,519 |
University of Southern Mississippi |
2006-05-16 |
[SAGE NlaIII] SAGE:17:NlaIII:Palaemonetes pugio (GPL3770) |
SAGE |
Palaemonetes pugio
 |
unclassified |
source_name:Grass shrimp Cadmium-exposed title:Grass Shrimp Cadmium-exposed Library description:This library was constructed from Cadmium-exposed shrimp in an exposure to identify differentially expressed genes in shrimp following exposure to fipronil, endosulfan, or cadmium. |
| 12 |
GSM109147 |
Grass Shrimp Fipronil-exposed Library |
3,189 |
University of Southern Mississippi |
2006-05-16 |
[SAGE NlaIII] SAGE:17:NlaIII:Palaemonetes pugio (GPL3770) |
SAGE |
Palaemonetes pugio
 |
unclassified |
source_name:Grass shrimp Fipronil-exposed title:Grass Shrimp Fipronil-exposed Library description:This library was constructed from Fipronil-exposed shrimp in an exposure to identify differentially expressed genes in shrimp following exposure to fipronil, endosulfan, or cadmium. |
| 13 |
GSM119465 |
Bombyx ovary SAGE library #1 |
40,311 |
The University of Tokyo |
2006-07-10 |
[SAGE NlaIII] SAGE:16:NlaIII:Bombyx mori (GPL3984) |
SAGE |
Bombyx mori
 |
ovary |
ovary (stage1-stage3) |
| 14 |
GSM119467 |
Bombyx ovary SAGE library #2 |
16,740 |
The University of Tokyo |
2006-07-10 |
[SAGE NlaIII] SAGE:16:NlaIII:Bombyx mori (GPL3984) |
SAGE |
Bombyx mori
 |
ovary |
ovary (stage2-3) |
| 15 |
GSM87437 |
Leishmania donovani promastigotes |
8,615 |
Shanghai Jiao Tong University |
2005-12-12 |
[SAGE NlaIII] SAGE:10:NlaIII:Leishmania donovani (GPL3252) |
SAGE |
Leishmania donovani
 |
peripheral blood |
L. donovani promastigotes were routinely cultured at 22℃ in M199 medium(pH 7.4) supplemented with 10% heat-inactivated fetal bovine serum |
| 16 |
GSM282623 |
Leishmania donovani axenic amastigotes |
7,835 |
Shanghai Jiao Tong University |
2008-04-18 |
[SAGE NlaIII] SAGE:10:NlaIII:Leishmania donovani (GPL3252) |
SAGE |
Leishmania donovani
 |
unclassified |
source_name:Leishmania donovani title:Leishmania donovani axenic amastigotes description:Amastigotes were cultured in a 95% air:5% CO2 humid atmosphere ratio in Medium 199 supplemented with 20% (V/V) FBS, pH 5.5, 37℃. |
| 17 |
GSM51910 |
SAGE library generated from normal Bombyx. mori embryos |
17,718 |
Shanghai Institute of Plant Physiology ang Ecology |
2005-05-19 |
[SAGE NlaIII] SAGE:10:NlaIII:Bombyx mori (GPL2095) |
SAGE |
Bombyx mori
 |
embryo |
mix of the developing embryos |
| 18 |
GSM51912 |
radiation treated Bombyx Mori developing embryos |
15,531 |
Shanghai Institute of Plant Physiology ang Ecology |
2005-05-19 |
[SAGE NlaIII] SAGE:10:NlaIII:Bombyx mori (GPL2095) |
SAGE |
Bombyx mori
 |
embryo |
mix of the radiation developing embryos |
| 19 |
GSM652443 |
LongSAGE Leishmania infantum chagasi |
8,016 |
INSTITUTO DE DOENÇAS TROPICAIS NATAN PORTELLA |
2011-01-10 |
[SAGE NlaIII] SAGE:17:NlaIII:Leishmania chagasi (GPL11414) |
SAGE |
Leishmania chagasi
 |
unclassified |
source_name:Leishmania infantum chagasi promastigote cells title:LongSAGE Leishmania infantum chagasi description:leishmania infantum chagasi is the causative agent of visceral leishmaniasis in the New World. |
| 20 |
GSM264128 |
Metacyclic Leishmanaia major parasites |
9,530 |
Institut Pasteur de Tunis |
2008-02-07 |
[SAGE NlaIII] SAGE:10:NlaIII:Leishmania major (GPL6471) |
SAGE |
Leishmania major
 |
unclassified |
source_name:Metacyclic Leishmania major parasites title:Metacyclic Leishmanaia major parasites description:Metacyclic Leishmanaia major parasites |