||Yale University School of Medicine
||spotted DNA/cDNA, Dmel_arrayplatform, Spotted array using PCR fragments of approximately 95% of the genes in the D. melanogaster BDGP genome. We followed a sequence of decreasingly stringent tests to pick optimal primer pairs for the PCR fragments on the arrays. For each gene, we looked for (first) a 500b to 2kb sequence at the 3Ã¢â‚¬â„¢ end and, failing this, (second) two 200b to 500b sequences in:(1) coding sequence, completely within an EST clot, and unduplicated (BLAST<=10^-4); (2) coding sequence, partially within an EST clot, unduplicated; (3) coding sequence, unduplicated; (4) exon, completely within an EST clot, unduplicated; (5) exon, partially within an EST clot, unduplicated; (6) exon, unduplicated; (7) sequence less than 2kb at the 3Ã¢â‚¬â„¢ end. See above for the other details about making these arrays.