||WUSTL Brugia malayi v.1
||Washington Univeristy School of Medicine
||spotted oligonucleotide, WUSTL Brugia malayi v.1, B. malayi clusters for arrays were selected from 8392 clusters generated and posted at the website http://nema.cap.ed.ac.uk/nematodeESTs/nembase These clusters were derived from > 20 cDNA libraries that represent the major B. malayi life cycle stages. They represent approximately ~40% of the total number of predicted genes for B. malayi. Clusters with multiple ESTs, predicted homology by BLAST, and sequence permitting design of a unique 65-mer oligonucleotides were chosen for inclusion on the array. Oligos were synthesized from the consensus sequence of selected clusters (n=3569) by standard methods by Illumina (San Diego, CA). The oligonucleotides (50nM in 3x SSC with 0.75M betaine) were printed in duplicate on MWG Epoxy slides (MWG Biotech Inc, High point, NC) by a locally constructed linear servo arrayer (after the DeRisi model, http://derisilabs.ucsf.edu/ ). Keywords = brugia malayi nematode worm parasite filaria filariasis
||Fred Hutchinson Cancer Research Center
||spotted DNA/cDNA, dmyc overexpression, cDNA spotted microarrays were constructed from release 1 of the Drosophila Gene Collection (Rubin et al. 2000 ) and 430 additional cDNA and genomic sequences. ). Microarray analyses were performed on samples ar 7 or 14h after dMyc induction. Total RNA was isolated from wondering third instar larvae (120 h after egg deposition, AED) which were heat shocked at 37Ã‚Â°C for 2 h.
||cDNA microarray Trymanosoma cruzi - v.2
||Universidade de SÃƒÂ£o Paulo
||spotted DNA/cDNA, cDNA microarray Trymanosoma cruzi - v.2, DNA microarrays were constructed with 755 ESTs obtained from non-normalised and normalised cDNA libraries of CL Brener epimastigotes and from amastigotas of Tulahuen (ÃƒÅ“rmenyi et al., 1999; DaRocha et al., 2000). Polymerase chain reaction (PCR) amplification of the ESTs (average length 800 bp) was obtained with T3 and T7 primers using DNA polymerase (Biolase). The amplification products were purified with Multiscreen plates (Millipore). Inserts representing 32 cloned T. cruzi coding and non-coding genes, control sheared DNAs and oligonucleotides were also immobilised on the glass slides with Generation III Microarray System spotter (Molecular Dynamics) according to manufacturer's instructions.