| 1 |
GPL1473 |
CDMC_Drosophila_7k3 |
16,896 |
University of Toronto |
2004-09-28 |
|
Drosophila melanogaster
 |
cDNA Array |
spotted DNA/cDNA, CDMC_Drosophila_7k3, The CDMC_Drosophila_7k3 array is a primarily cDNA-based Drosophila melanogaster glass microarray. The array features 5,756 Berkeley Drosophila genome Project cDNAs, 1,078 NIH Testis cDNAs (Andrews et al., 2000) and 546 gene fragments that were amplified from genomic DNA, together representing approximately 50% of the predicted genes in D. melanogaster. The array is produced by the Canadian Drosophila Microarray Centre, located at the University of Toronto, and is available for distribution to academic labs. The arrays are printed on CMT-GAPS II substrates (Corning Inc., Acton, MA) using a SpotArray 72 microarrayer (Perkin Elmer, Boston, MA). The array features 16,896 spots in total, representing ~6,900 unique genes and numerous controls. The spots are approximately 125 microns in diameter and are spaced at 180 micron centre-to-centre distances. Printing conditions and post-print treatments are as described earlier (Neal et al., 2003). Keywords = CDMC Keywords = Drosophila Keywords = Microarray Keywords = cDNA |
| 2 |
GPL1467 |
CDMC_Drosophila_12k1 |
27,648 |
University of Toronto |
2004-09-26 |
|
Drosophila melanogaster
|
cDNA Array |
spotted DNA/cDNA, CDMC_Drosophila_12k1, The 12k1 platform is a primarily cDNA-based glass microarray. The array features 11,018 Berkeley Drosophila Genome Project cDNAs, 297 NIH Testis cDNAs (Andrews et al., 2000) and 432 gene sequences that were amplified from genomic DNA. Approximately 10,500 unique genes are represented by the above, corresponding to roughly 78% of the predicted genes in Drosophila melanogaster (FlyBase annotation release 3.2). This array is produced by the Canadian Drosophila Microarray Centre located at the University of Toronto. It is available for distribution to academic labs. The 12k1 arrays are printed on CMT-UltraGAPS substrates (Corning Inc, Acton, MA) using a SpotArray 72 microarrayer (Perkin Elmer, Boston, MA). There are 26880 spots and 768 blanks on each array in a 12 row by 4 column arrangement of blocks. Each block contains 560 spots and 16 blanks arrayed in a 24 row by 24 column grid. The blanks occupy positions 9 through 24 of the 24th row. The blocks are spaced at 4500 micron intervals and the spot-to-spot distance is 180 microns. The nominal spot diameter is 125 microns. Printing conditions and post-print treatments are as described earlier (Neal et al., 2003). Keywords = CDMC Keywords = Drosophila Keywords = microarray Keywords = cDNA |
| 3 |
GPL1447 |
Drosophila 200K high density 36mer oligo array |
193,866 |
Yale |
2004-09-14 |
|
Drosophila melanogaster
|
Oligo Array |
spotted oligonucleotide, Drosophila 200K high density 36mer oligo array, synthesized oligonucleotide array on glass. This set includes 200,000 oligonucleotides, uniquely identified 36-mers. Keywords = high density oligonucleotide |
| 4 |
GPL1404 |
MJArray Fly |
32,448 |
Forschungszentrum Karlsruhe |
2004-08-11 |
|
Drosophila melanogaster
|
cDNA Array |
spotted DNA/cDNA, MJArray Fly, The array was assembled using amplicons that were obtained using the Incyte primer set. The set is identical to the one used to produce platform GPL20, primer sequences can be viewed in that platform. We included a reference to the ID of GPL20. The amplicons represent approximately 93% of the genes predicted in version 1.0 of the Drosophila genome from sequences released by the Berkeley Drosophila Genome Project and Celera Genomics in March 2000. The amplicons represent approximately 75% of the genes predicted in the January 2003 release 3.1 Drosophila genome annotation update. The regions amplified to build the array ranged in size from 150 to 600 bp with an average size of 410 bp. PCR fragments were amplified and each amplicon was quality control tested for DNA concentration, purity, and fragment size. A total of 11,796 reactions led to a single product of correct size and sufficient amount (marked as ok in column GENE_NAME). The 675 reactions that produced multiple products were run on a gel again and the band of the correct size was excised cloned into pCR TOPO 2.1 (Invitrogen). The 2034 reactions where no band was visible was also subjected to a 'blind' clone-attempt. Those 2709 cloning-reactions led to 2033 positive clones, that were included on the array and marked as 'clone!' in column GENE_NAME. Non sequence-verified clones were not included in the current study. A mixture of all these 2033 clones was used to make a titration dilution as a hybridisation control (Incyte Amplification Titration Standard, IATS) that is included in each of the 48 blocks of our array. Arrays were spotted with a Omnigrid 100 (GeneMachines) with 24 SMP3 pins (Telechem). By having two identical subarrays next to each other on each slide, we obtain 48 blocks with 26 rows and 26 columns each. Spot to spot spacing is 150 micron, average spot diameter is 100 micron. |
| 5 |
GPL1321 |
[Plasmodium_Anopheles] Affymetrix Plasmodium/Anopheles Genome Array |
22,769 |
Affymetrix, Inc. |
2004-07-01 |
Affymetrix |
Plasmodium falciparum,Anopheles gambiae
 |
GeneChip |
in situ oligonucleotide, [Plasmodium_Anopheles] Affymetrix Plasmodium/Anopheles Genome Array, Affymetrix submissions are typically submitted to GEO using the GEOarchive method described at http://www.ncbi.nlm.nih.gov/projects/geo/info/geo_affy.html June 03, 2009: annotation table updated with netaffx build 28 Oct 12, 2009: annotation table updated with netaffx build 32 |
| 6 |
GPL1322 |
[Drosophila_2] Affymetrix Drosophila Genome 2.0 Array |
18,952 |
Affymetrix, Inc. |
2004-07-01 |
Affymetrix |
Drosophila melanogaster
|
GeneChip |
in situ oligonucleotide, [Drosophila_2] Affymetrix Drosophila Genome 2.0 Array, Affymetrix submissions are typically submitted to GEO using the GEOarchive method described at http://www.ncbi.nlm.nih.gov/projects/geo/info/geo_affy.html June 03, 2009: annotation table updated with netaffx build 28 June 08, 2012: annotation table updated with netaffx build 32 |
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