| 1 |
GPL223 |
SAGE:10:NlaIII:Bos taurus |
115,184 |
|
2002-12-04 |
|
Bos taurus
 |
SAGE NlaIII |
SAGE NlaIII, SAGE:10:NlaIII:Bos taurus, |
| 2 |
GPL245 |
BMAM microarray |
3,888 |
Michigan State University |
2003-02-06 |
|
Bos taurus
|
cDNA Array |
spotted DNA/cDNA, BMAM microarray, Bovine cDNA microarray enhanced for mammary transcripts. Keywords = cDNA, microarray, mammary, bovine, lactation, involution |
| 3 |
GPL309 |
NBFGC_1 |
19,194 |
Michigan State University |
2003-04-24 |
|
Bos taurus
|
cDNA Array |
spotted DNA/cDNA, NBFGC_1, National Bovine Functional Genomics Consortium microarray version 1 Keywords = bovine microarray EST cDNA |
| 4 |
GPL325 |
NBFGC cDNA microarray |
19,200 |
Michigan State University |
2003-05-15 |
|
Bos taurus
|
cDNA Array |
spotted DNA/cDNA, NBFGC cDNA microarray, National Bovine Functional Genomics Consortium cDNA microarray. Keywords = cDNA, microarray, bovine |
| 5 |
GPL336 |
Porcine Brain Library array |
3,888 |
Michigan State University |
2003-06-12 |
|
Sus scrofa
 |
cDNA Array |
spotted DNA/cDNA, Porcine Brain Library array, cDNA microarray from porcine brain cDNA library |
| 6 |
GPL338 |
BLI_array_NIAS |
9,216 |
National Institute of Agrobiological Sciences |
2003-06-17 |
|
Bos taurus
|
cDNA Array |
spotted DNA/cDNA, BLI_array_NIAS, BLI_array_NIAS platform of a spotted cDNA microarray has been established using a bovine liver cDNA library. The array has been fabricated collaboratively by Laboratory of Reproductive Biology and Technology at National Institute of Agrobiological Sciences, Tsukuba Science City, Japan and Department of Paediatric Pharmacology at National Institute of Child Health and Development, Tokyo, Japan. Keywords = bovine liver, cDNA array, gene expression |
| 7 |
GPL363 |
BOTL3 7_18_03 |
3,888 |
Michigan State University |
2003-07-18 |
|
Bos taurus
|
cDNA Array |
spotted DNA/cDNA, BOTL3 7_18_03, Bovine Total Leukocyte cDNA microarray Third Generation Microarray Keywords = microarray, cDNA, bovine |
| 8 |
GPL374 |
ToxExpress GeneChip Canine Array |
12,505 |
University of Pennsylvania |
2003-08-05 |
|
Canis lupus familiaris
 |
GeneChip |
in situ oligonucleotide, ToxExpress GeneChip Canine Array, Affymetrix Canis familiaris array. Available commercially from GeneLogic Bethesda, MD. |
| 9 |
GPL518 |
UIUC Porcine muscle plus |
2,880 |
University of Illinois at Urbana Champaign |
2003-10-02 |
|
Sus scrofa
|
cDNA Array |
spotted DNA/cDNA, UIUC Porcine muscle plus, cDNA microarray from porcine muscle cDNA library |
| 10 |
GPL974 |
Dog heart cDNA |
2,304 |
Louis Bugnard |
2004-02-04 |
|
Canis lupus familiaris
|
cDNA Array |
spotted DNA/cDNA, Dog heart cDNA, DNA microarray from substractive cDNA library of dog heart Keywords = Heart dog obesity hypertension |
| 11 |
GPL1209 |
Porcine 1000 embryo gene array |
1,015 |
Iowa State University |
2004-04-30 |
|
Sus scrofa
|
cDNA Array |
spotted DNA/cDNA, Porcine 1000 embryo gene array, Porcine cDNA microarray, containing 1021 genes selected for embryo expression. This platform includes the GenBank accession numbers, our clone ID, and gene name description for the 1021 genes. |
| 12 |
GPL1221 |
BPL_array_NIAS |
4,608 |
National Institute of Agrobiological Sciences |
2004-05-07 |
|
Bos taurus
|
cDNA Array |
spotted DNA/cDNA, BPL_array_NIAS, BPL_array_NIAS platform of a spotted cDNA microarray has been established using a bovine utero-placenta cDNA library. The array has been fabricated collaboratively by Laboratory of Reproductive Biology and Technology at National Institute of Agrobiological Sciences, Tsukuba City, Japan and Department of Paediatric Pharmacology at National Institute of Child Health and Development, Tokyo, Japan. Keywords = bovine utero-placenta Keywords = cDNA array Keywords = gene expression |
| 13 |
GPL1270 |
SAGE:10:NlaIII:Sus scrofa |
137,047 |
|
2004-06-01 |
|
Sus scrofa
|
SAGE NlaIII |
SAGE NlaIII, SAGE:10:NlaIII:Sus scrofa, This is a virtual platform for SAGE libraries generated for Sus scrofa using NlaIII as an anchor enzyme. Platform_anchor: NlaIII |
| 14 |
GPL1434 |
10K AgR Bovine Array |
10,752 |
AgResearch |
2004-09-02 |
|
Bos taurus
|
cDNA Array |
spotted DNA/cDNA, 10K AgR Bovine Array, This array contains spotted PCR products from our bovine EST collection. Included are housekeeping genes, water blanks (negative PCR) and no DNA blanks (to estimate carryover). |
| 15 |
GPL1489 |
Canis Familiaris Brain |
4,224 |
NCSU |
2004-10-11 |
|
Canis lupus familiaris
|
cDNA Array |
spotted DNA/cDNA, Canis Familiaris Brain, Probe set derived from Canine brain EST library |
| 16 |
GPL1527 |
CattleArray3800 from PyxisGenomics |
8,064 |
University of Minnesota |
2004-10-22 |
|
Bos taurus
|
cDNA Array |
spotted DNA/cDNA, CattleArray3800 from PyxisGenomics, The array elements were selected from cDNA clones derived from expressed sequence tags (ESTs) of bovine spleen and normalized placenta libraries. The ESTs representing distinct genes were determined via sequence similarity searches against human UniGene sequences using an E value of e10-5 as a threshold. The CattleArray3800 contains positive expression controls: beta actin (ACTB); phosphoglycerate kinase 1; (PGK1); hypoxanthine phosphoribosyltransferase (HPRT) and beta-2-microglobulin (B2M). Two exogenous plant genes, soy rubisco small chain 1 (RBS1) and chlorophyll ab binding protein (CAB) can be used as negative or spiking controls. |
| 17 |
GPL1624 |
PorkChip 2,600 cDNA array |
12,288 |
University of Minneosta |
2004-10-29 |
|
Sus scrofa
|
RT-PCR |
spotted DNA/cDNA, PorkChip 2,600 cDNA array, Spotted cDNA array on glass. This set includes approximately 2,600 cDNA clones, selected from a Peyer’s patch subtracted library and other sources. The PorkChip2 cDNA microarray contains 2,423 ESTs from the subtracted jejunal Peyer’s patch library (UMNMPM3), 10 ESTs from the normal jejunal Peyer’s patch library (UMNMPM1), and 26 ESTs from the immune-stimulated jejunal Peyer’s patch library (UMNMPM2), which were cloned using the GeneTrapper cDNA Positive Selection System and pCMVSport6 vector (Invitrogen Corporation, Carlsbad, CA) (2). Each array also contains 86 ESTs identified by differential display RT-PCR (DD-RT-PCR) of PRRSV-infected macrophages (Library: PRRSV DD-RT-PCR)(5), 19 ESTs identified by DD-RT-PCR of influenza-infected porcine epithelial PK-15 cells (Library: UMNMPM4), 40 porcine genes, which were the result of directed cloning efforts (Library: Sus scrofa gene), and 7 porcine reproductive and respiratory syndrome virus strain VR-2332 sequences (Library: PRRSV) positive and negative controls (Library: Control). Positive controls included 8 Ambion ArrayControl sequences. The plasmid inserts were amplified by two rounds of nested PCR using vector-specific primers. The products from the second round PCR were cleaned by the Montage PCR(96) kit (Millipore, Billerica, MA) and were run on 0.8% agarose gels to quantify the amount of DNA by comparison to standards run on each gel. The dried PCR products were resuspended in phosphate buffer (150 mM sodium phosphate and 1 mM EDTA (pH 8.5)) at a final concentration of 100-300 ng/microliter and transferred to 384 well plates. A BioRobotics MicroGrid II (BioRobotics Ltd., Haslingfield, Cambridge, UK) arrayed the DNA on slides coated with poly-L-lysine (Sigma, St. Louis, MO)(3). Elements were arranged in 48 subarrays of 16 X 16, for a total of 12,288 spots per microarray, with each clone represented in by 4 spots. The slides were baked for 15 min at 80 C, UV irradiated (65 mJ), and chemically blocked with 1-methyl-2-pyrrolidinone and succinic anhydride (4) and were stored at room temperature under vacuum until use. The quality of each printing run was verified by staining one slide with SYBR Gold (Molecular Probes, Eugene, OR) and scanning at 488/530 nm (Excitation/ Emission) (1). Platform_references: 1. Battaglia C, Salani G, Consolandi C, Bernardi LR, and De Bellis G. Analysis of DNA microarrays by non-destructive fluorescent staining using SYBR green II. Biotechniques 29: 78-81, 2000. 2. Dvorak CMT, Hyland KA, Machado JG, Zhang Y, Fahrenkrug SC, and Murtaugh MP. Gene discovery and expression profiling in porcine Peyer's patch. Vet Immunol Immunopathol, submitted, 2004. 3. Eisen MB and Brown O. DNA arrays for analysis of gene expression. Methods Enzymol 303: 179-205, 1999. 4. Shalon D, Smith SJ, and Brown PO. A DNA microarray system for analyzing complex DNA samples using two-color fluorescent probe hybridization. Genome Res 6: 639-645, 1996. 5. Zhang X, Shin J, Molitor TW, Schook LB, and Rutherford MS. Molecular responses of macrophages to porcine reproductive and respiratory syndrome virus infection. Virology 262: 152-162, 1999. Keywords = small intestine, cDNA, pig |
| 18 |
GPL1854 |
24K AgR Bovine Array |
24,192 |
AgResearch |
2005-02-06 |
|
Bos taurus
|
cDNA Array |
spotted DNA/cDNA, 24K AgR Bovine Array, This array contains spotted PCR products from our bovine EST collection. Included are housekeeping genes, water blanks (negative PCR) and no DNA blanks (to estimate carryover). |
| 19 |
GPL1881 |
Qiagen-NRSP-8 porcine oligo array |
13,677 |
Iowa State University |
2005-02-23 |
|
Sus scrofa
|
Oligo Array |
spotted oligonucleotide, Qiagen-NRSP-8 porcine oligo array, To develop broadly useful tools in porcine functional genomics, Qiagen-Operon and the USDA-NRSP-8 Swine Genome community collaborated to develop a novel 13K oligonucleotide set (porcine AROS 1.0/1.0 Extension; www.qiagen.com) . The Qiagen oligonucleotide set represents porcine cDNAs and EST, designed from TIGR Tentative Consensus cDNA sequences. These 70-mer oligonucleotides were designed to minimize cross-hybridization and to be as specific to the gene of interest as possible. Keywords = Pig Keywords = oligonucleotide microarray Keywords = tissue Keywords = infection Keywords = expression |
| 20 |
GPL2108 |
Cattle 7,872-element cDNA |
16,896 |
University of Illinois |
2005-05-20 |
|
Bos taurus
|
cDNA Array |
spotted DNA/cDNA, Cattle 7,872-element cDNA, The 7,872 cDNAs used in this microarray were obtained from normalized and subtracted cattle placenta and spleen cDNA libraries (see Band et al., 2002 Anim. Biotechnol. 13:163-172; Everts et al., 2005 Vet. Immunol. Immunopathol. 105:235-245). Positive controls include beta actin (ACTB), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), and hypoxanthine phosphoribosyltransferase (HPRT). Exogenous spiking controls are the soybean genes chlorophyll ab binding protein (CAB), Rubisco small chain 1 (RBS1), and major latex protein (MSG). Negative controls are Cot1 DNA, genomic DNA, spotting buffer, poly-A, and water. All PCR products were printed in duplicate on the array. Keywords: cDNA, cow, liver, bovine, lactation, microarray |
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