| 1 |
GPL2864 |
UIUC Cattle 7,872-element cDNA - alternate version |
16,416 |
University of Illinois |
2005-09-22 |
W.M. Keck Center, University of Illinois Urbana-Champaign |
Bos taurus
 |
cDNA Array |
spotted DNA/cDNA, UIUC Cattle 7,872-element cDNA - alternate version, The 7,872 cDNAs used in this microarray were obtained from normalized and subtracted cattle placenta and spleen cDNA libraries (see Band et al., 2002 Anim. Biotechnol. 13:163-172; Everts et al., 2005 Vet. Immunol. Immunopathol. 105:235-245). Positive controls include beta actin (ACTB), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), and hypoxanthine phosphoribosyltransferase (HPRT). Exogenous spiking controls are the soybean genes chlorophyll ab binding protein (CAB), Rubisco small chain 1 (RBS1), and major latex protein (MSG). Negative controls are Cot1 DNA, genomic DNA, spotting buffer, poly-A, and water. All PCR products were printed in duplicate on the array. This is an alternative version of GPL2108. Keywords: cDNA, cow, bovine, microarray |
| 2 |
GPL2672 |
Gene expression profiling of 19 cattle tissues reveals unique patterns related to tissue function |
16,128 |
University of Illinois |
2005-07-24 |
University of Illinois |
Bos taurus
|
cDNA Array |
spotted DNA/cDNA, Gene expression profiling of 19 cattle tissues reveals unique patterns related to tissue function, TeleChem superamine slides |
| 3 |
GPL2731 |
Spotting_muscle_21OCT03 |
4,608 |
INRA |
2005-08-09 |
RGS platform of Genopole Toulouse Midi Pyrénées (http://genopole-toulouse.prd.fr/) and UR 444 Cellular Genetics INRA, chemin de Borde Rouge Auzeville, BP 52627, 31326 Castanet-Tolosan cedex, France |
Sus scrofa
 |
cDNA Array |
spotted DNA/cDNA, Spotting_muscle_21OCT03, |
| 4 |
GPL2788 |
LAFUGA Cattle subtr lib 18KB v1.0 |
1,728 |
Ludwig-Maximilians University of Munich |
2005-08-26 |
Laboratory for Functional Genome Analysis (LAFUGA), Gene Center, Ludwig-Maximilians University of Munich |
Bos taurus
|
cDNA Array |
spotted DNA/cDNA, LAFUGA Cattle subtr lib 18KB v1.0, Pretreatment of Animals and Collection of Endometrial Tissue Samples for the production of subtracted cDNA libraries: Ten cyclic heifers (Deutsches Fleckvieh, Simmental) between 18 and 24 months old were cycle synchronized by injecting intramuscularly a single dose of 500 µg Cloprostenol (Estrumate®; Essex Tierarznei, Munich, Germany) at dioestrus. Animals were observed for sexual behaviour (i.e., toleration, sweating, vaginal mucus) to determine standing heat, which occurred around 60 h after Estrumate injection. In addition, animals were checked with ultrasound-guided follicle monitoring. Blood samples were taken at day 20, day 0 of the estrous cycle and just before slaughtering to determine serum progesterone (P4) levels. The first group of animals was inseminated with cryo-conserved sperm (ejaculate + diluter 1:10) at estrus. Animals were slaughtered at Day 18 of gestation and intact concepti were detected in the ipsilateral uterine horns of the animals used for gene expression analysis (n=4). Control animals (n=6) were inseminated with the supernatant of centrifuged sperm and also slaughtered at day 18 of the estrous cycle to achieve an identical as possible pretreatment of both groups. No conceptus was found in control animals. Sperm was derived from the same bull. The uterus was prepared and opened longitudinally. In pregnant animals, the conceptus was localized and carefully removed. Samples were carefully cut out from the lamina propria of the intercaruncular endometrium with a scalpel and immediately transferred into an appropriate amount of RNAlater (Ambion). Tissue sample of the caudal part of the ipsilateral uterine horn were used for transcriptome analyses.Samples were stored at -20°C until further processing. Generation of Subtracted cDNA Libraries: The production of subtracted libraries was done according to the Suppression Subtractive Hybridization (SSH) method (Diatchenko et al. 1996). Total RNA from endometrial tissue samples of the ipsilateral caudal part of the uterus was isolated using Trizol reagent (Invitrogen, Karlsruhe, Germany) according to the manufacturer's recommendations. The quantity and quality of total RNA was determined by spectrometry and agarose gel electrophoresis. Sixty microgram of pooled total RNA (corresponding to approximately 1.8 µg mRNA) were used for the preparation of subtracted cDNA libraries for pregnant and control animals, respectively. The construction of the libraries was done as previously described (Bauersachs S et al. 2003; Bauersachs S et al. 2004) with the modification that no additional driver cDNA was added at the second step of subtractive hybridization. For every library 1,536 cDNA clones were randomly picked. This array (LAFUGA Cattle subtr lib 18KB v1.0) contains the cDNA clones derived from the subtracted library (18KB) enriched for cDNAs of genes upregulated in endometrium of Day 18 pregnant animals. |
| 5 |
GPL2792 |
LAFUGA Cattle subtr lib 18KO v1.0 |
1,728 |
Ludwig-Maximilians University of Munich |
2005-08-26 |
Laboratory for Functional Genome Analysis (LAFUGA), Gene Center, Ludwig-Maximilians University of Munich |
Bos taurus
|
cDNA Array |
spotted DNA/cDNA, LAFUGA Cattle subtr lib 18KO v1.0, Pretreatment of Animals and Collection of Endometrial Tissue Samples for the production of subtracted cDNA libraries: Ten cyclic heifers (Deutsches Fleckvieh, Simmental) between 18 and 24 months old were cycle synchronized by injecting intramuscularly a single dose of 500 µg Cloprostenol (Estrumate®; Essex Tierarznei, Munich, Germany) at dioestrus. Animals were observed for sexual behaviour (i.e., toleration, sweating, vaginal mucus) to determine standing heat, which occurred around 60 h after Estrumate injection. In addition, animals were checked with ultrasound-guided follicle monitoring. Blood samples were taken at day 20, day 0 of the estrous cycle and just before slaughtering to determine serum progesterone (P4) levels. The first group of animals was inseminated with cryo-conserved sperm (ejaculate + diluter 1:10) at estrus. Animals were slaughtered at Day 18 of gestation and intact concepti were detected in the ipsilateral uterine horns of the animals used for gene expression analysis (n=4). Control animals (n=6) were inseminated with the supernatant of centrifuged sperm and also slaughtered at day 18 of the estrous cycle to achieve an identical as possible pretreatment of both groups. No conceptus was found in control animals. Sperm was derived from the same bull. The uterus was prepared and opened longitudinally. In pregnant animals, the conceptus was localized and carefully removed. Samples were carefully cut out from the lamina propria of the intercaruncular endometrium with a scalpel and immediately transferred into an appropriate amount of RNAlater (Ambion). Tissue sample of the caudal part of the ipsilateral uterine horn were used for transcriptome analyses.Samples were stored at -20°C until further processing. Generation of Subtracted cDNA Libraries: The production of subtracted libraries was done according to the Suppression Subtractive Hybridization (SSH) method (Diatchenko et al. 1996). Total RNA from endometrial tissue samples of the ipsilateral caudal part of the uterus was isolated using Trizol reagent (Invitrogen, Karlsruhe, Germany) according to the manufacturer's recommendations. The quantity and quality of total RNA was determined by spectrometry and agarose gel electrophoresis. Sixty microgram of pooled total RNA (corresponding to approximately 1.8 µg mRNA) were used for the preparation of subtracted cDNA libraries for pregnant and control animals, respectively. The construction of the libraries was done as previously described (Bauersachs S et al. 2003; Bauersachs S et al. 2004) with the modification that no additional driver cDNA was added at the second step of subtractive hybridization. For every library 1,536 cDNA clones were randomly picked. This array (LAFUGA Cattle subtr lib 18KO v1.0) contains the cDNA clones derived from the subtracted library (18KO) enriched for cDNAs of genes downregulated in endometrium of Day 18 pregnant animals compared to non-pregnant controls. |
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