| 1 |
GPL7417 |
INRA-BDR Bovine 10k |
10,368 |
INRA |
2008-10-02 |
INRA/UMR Biologie du Developpement et Reproduction |
Bos taurus
 |
cDNA Array |
spotted DNA/cDNA, INRA-BDR Bovine 10k, (22cmx22cm, Amersham Biosciences) - 3x3 pattern - The 10K custom cDNA membrane developed in our laboratory was used for the experiment. Briefly, the array contained 7.800 EST from term placenta (Everts et al., 2005) and we incremented this set by 2.400 new EST from a cDNA library established on young foetuses and early extra-embryonic tissues. This second library (called BE03) was constructed from mRNA isolated from extra-embryonic tissues of bovine embryos from D14 to D24 of gestation and foetuses (D36 and D64). The BE03 library was normalized and subtracted by the 7.8K set using the protocol from Bento Soares. About 10.000 bacterial clones were sequenced. The sequences were repeat-masked using RepeatMasker (05/05/99) and the clustering was performed by CAP3. The CAP3 assembly resulted into 2.400 new EST. In total, 10.214 unique cDNA were spotted onto Nylon N+ membranes (Amersham Biosciences) with a 3x3 pattern (QBot, Proteigene) at the CRB GADIE platform (INRA, Jouy en Josas, France). The “BE03†library is indexed as Lib. 17188 and Lib. 15992 (http://www.ncbi.nlm.nih.gov). |
| 2 |
GPL8016 |
MacLeod custom equine cartilage cDNA microarray, Version 2 |
9,405 |
University of Kentucky |
2008-12-31 |
W.M. Keck Center, University of Illinois Urbana-Champaign |
Equus caballus
 |
cDNA Array |
spotted DNA/cDNA, MacLeod custom equine cartilage cDNA microarray, Version 2, The 9,322 cDNAs spotted on this microarray were obtained from a normalized and subtracted cDNA library generated from the total RNA of articular cartilage from a 15-month old equine Thoroughbred gelding. In addition, cDNAs from 28 plasmids used within the lab were spotted; 2 plasmids have been submitted to GenBank. Positive control spots include Elongation Factor Tu (EFTu) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH). Negative control spots are Invitrogen oligos from Arabidopsis thaliana genes CAD1 and GAPC. The original negative control soybean genes chlorophyll ab binding protein (CAB - SPOT_ID: ABbinding) and Rubisco small chain 1 (RBS1 - SPOT_ID: Rubisco) were contaminated prior to spotting with Bos taurus cDNA and thus might show hybridization. Other spots might be identified as empty of buffer. Keywords: cDNA, equine, articular cartilage, microarray |
| 3 |
GPL7671 |
Bovine oviduct and endometrium (BOE) 1.7K cDNA array version 1.2 |
1,728 |
Ludwig-Maximilians University of Munich |
2008-11-19 |
Laboratory for Functional Genome Analysis (LAFUGA) - Genomics Unit, Gene Center of the LMU Munich, Munich, Germany |
Bos taurus
 |
cDNA Array |
spotted DNA/cDNA, Bovine oviduct and endometrium (BOE) 1.7K cDNA array version 1.2, |
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