||University of British Columbia
||spotted oligonucleotide, GBC_FOAR03_0001, The Arabidopsis Genome Oligo Set Version 1.0 (Operon Biotechnologies, Huntsville, AL, USA) consists of 26090 70mer oligonucleotides (http://operon.com/arrays/oligosets_arabidopsis.php). As positive controls, we included oligos for twelve housekeeping genes (Operon). As negative controls, we synthesized four oligonucleotides specific for human genes with no similarity to any Arabidopsis gene and included twelve oligonucleotides with no similarity to any Arabidopsis gene (Operon). We used a PCR amplified green fluorescent protein (GFP) cDNA (Invitrogen, Carlsbad, CA, USA) as an orientation marker. Oligonucleotides were resuspended in 384 well flat bottom plates (Nunc, Rochester, NY, USA) to a concentration of 100 mM in 3 x SSC. Oligos were printed on MicroGrid II robots using Microspot 10k pins (Biorobotics, Huntington, UK) depositing approximately 0.5 nl (0.0075 pmole) of each oligo onto EZ rays aminosilane slides (Apogent Discoveries, Hudson, NH, USA). The pitch of the grid used for this library was 0.2 mm. Oligos were UV cross linked at 3000 x 100 ÃŽÂ¼J using a UV Stratalinker 2400 (Stratagene, La Jolla, CA, USA). We spotted single spots for 25,792 of the 26090 target oligonucleotides (due to software problems the print run was terminated shortly before completion); in addition, each subgrid contained six replicate spots of each of the four human negative controls, three spots of equally distributed Operon negative controls, a single spot of each of the twelve house keeping controls, and GFP marker on each corner of the s Keywords = GenomeBC-Forestry Keywords = 26k array v.1
||The University of Iowa
||spotted oligonucleotide, Soll albicans, C. albicans Array-Ready Genome Oligo Set Version 1.1 (Qiagen, Valencia, CA) was resuspended at a concentration of 20uM in 150mM sodium phosphate buffer (pH 8.5). Oligos were printed in duplicate blocks on Code Link slides (Amersham Biosciences) at Microarray, Inc. DNA coupling and post-coupling processing of printed microarray slides were done according to the manufacturere's instructions (Amersham Biosciences).
||yeast intergenic array
||University of Geneva
||spotted oligonucleotide, yeast intergenic array, -Platform: amino-silane coated glass slides (GAPS II, Corning) -S. cerevisiae intergenic regions amplified from S288C genomic DNA (ResGen) using the intergenic region primer oligonucleotides (ResGen) (Harismendy et al. EMBO J. 22(18): 4738-4747, 2003). The primers allow the amplification of the sequence located on either side of elements such as open reading frames, tRNAs, small nuclear RNAs, Ty elements, solo ÃŽÂ´, etc. A complete description of the primers can be obtained on the web site (ftp://ftp.resgen.com/pub/genepairs/yeast_intergenic). -The PCR products were purified and spotted using an automated arrayer (MicroGrid II, BioRobotics)
||Custom Yeast Genomic 70-mer Oligonucleotide Microarray
||University of Illinois at Urbana-Champaign
||spotted oligonucleotide, Custom Yeast Genomic 70-mer Oligonucleotide Microarray, The custom microarrays consisted of OperonÃ¢â‚¬â„¢s Yeast Genomic 70-mer Oligonucleotide Set (Qiagen, Valencia, CA, v. 1.1) spotted in duplicate at a concentration of 20 mM in 150 mM sodium phosphate (pH 8.5), Arabidopsis oligonucleotide spike controls (Stratagene SpotReport, La Jolla, CA) spotted in quadruplicate, and 10 human and 10 yeast oligonucleotide negative controls spotted in duplicate. The oligonucleotides were printed on Codelink slides (Amersham, Piscataway, NJ) by Microarrays, Inc. (Nashville, TN). Post-print processing was conducted according to the manufacturerÃ¢â‚¬â„¢s recommendations.