spotted oligonucleotide, yeast intergenic array, -Platform: amino-silane coated glass slides (GAPS II, Corning) -S. cerevisiae intergenic regions amplified from S288C genomic DNA (ResGen) using the intergenic region primer oligonucleotides (ResGen) (Harismendy et al. EMBO J. 22(18): 4738-4747, 2003). The primers allow the amplification of the sequence located on either side of elements such as open reading frames, tRNAs, small nuclear RNAs, Ty elements, solo ÃŽÂ´, etc. A complete description of the primers can be obtained on the web site (ftp://ftp.resgen.com/pub/genepairs/yeast_intergenic). -The PCR products were purified and spotted using an automated arrayer (MicroGrid II, BioRobotics)
spotted oligonucleotide, Soll albicans, C. albicans Array-Ready Genome Oligo Set Version 1.1 (Qiagen, Valencia, CA) was resuspended at a concentration of 20uM in 150mM sodium phosphate buffer (pH 8.5). Oligos were printed in duplicate blocks on Code Link slides (Amersham Biosciences) at Microarray, Inc. DNA coupling and post-coupling processing of printed microarray slides were done according to the manufacturere's instructions (Amersham Biosciences).
SAGE NlaIII, SAGE:17:NlaIII:Magnaporthe grisea 70-15, This is a virtual platform for longSAGE 17 libraries generated for Magnaporthe grisea (stain 70-15) using NlaIII as an anchor enzyme. Platform_anchor: NlaIII
spotted DNA/cDNA, Rice Chromosome 4 Tilling-Path, The array represent a minimal tilling-path coving rice chromosome 4. This tilling-path was assembled using 14,742 randomly sheared shotgun subclone fragments. All subclone fragments were PCR amplified using universal primers and plasmids containing the genomic DNA fragment as the template. DNA gel analysis was performed for all fragments and poor amplification were not included here. In the final count by comparing with the TIGR version 2.0 sequence, 95.5% of the sequence of this chromosome is covered by the array. Keywords = rice Keywords = chromosome 4 Keywords = tilling-path
spotted DNA/cDNA, Pinus taeda cDNA, The microarray contains ESTs selected from 6 Xylem libraries: 1. NXCI - ESTs from juvenile compression wood of Pinus taeda (loblolly pine) 2. NXLV - ESTs from transitional/mature late wood of Pinus taeda (loblolly pine) 3. NXNV - ESTs from mature normal wood of Pinus taeda (loblolly pine) 4. NXPV - ESTs from transitional planings wood of Pinus taeda (loblolly pine) 5. NXRV - ESTs from root wood of Pinus taeda (loblolly pine) 6. NXSI - ESTs from juvenile side wood of Pinus taeda (loblolly pine) , a Pollen Cone library, and a Shoot tip library. The ESTs were PCR amplified, purified, denatured in 50% DMSO and printed on Corning GapsII amino-silane coated slides.
spotted oligonucleotide, GBC_FOAR03_0001, The Arabidopsis Genome Oligo Set Version 1.0 (Operon Biotechnologies, Huntsville, AL, USA) consists of 26090 70mer oligonucleotides (http://operon.com/arrays/oligosets_arabidopsis.php). As positive controls, we included oligos for twelve housekeeping genes (Operon). As negative controls, we synthesized four oligonucleotides specific for human genes with no similarity to any Arabidopsis gene and included twelve oligonucleotides with no similarity to any Arabidopsis gene (Operon). We used a PCR amplified green fluorescent protein (GFP) cDNA (Invitrogen, Carlsbad, CA, USA) as an orientation marker. Oligonucleotides were resuspended in 384 well flat bottom plates (Nunc, Rochester, NY, USA) to a concentration of 100 mM in 3 x SSC. Oligos were printed on MicroGrid II robots using Microspot 10k pins (Biorobotics, Huntington, UK) depositing approximately 0.5 nl (0.0075 pmole) of each oligo onto EZ rays aminosilane slides (Apogent Discoveries, Hudson, NH, USA). The pitch of the grid used for this library was 0.2 mm. Oligos were UV cross linked at 3000 x 100 ÃŽÂ¼J using a UV Stratalinker 2400 (Stratagene, La Jolla, CA, USA). We spotted single spots for 25,792 of the 26090 target oligonucleotides (due to software problems the print run was terminated shortly before completion); in addition, each subgrid contained six replicate spots of each of the four human negative controls, three spots of equally distributed Operon negative controls, a single spot of each of the twelve house keeping controls, and GFP marker on each corner of the s Keywords = GenomeBC-Forestry Keywords = 26k array v.1
spotted oligonucleotide, Custom Yeast Genomic 70-mer Oligonucleotide Microarray, The custom microarrays consisted of OperonÃ¢â‚¬â„¢s Yeast Genomic 70-mer Oligonucleotide Set (Qiagen, Valencia, CA, v. 1.1) spotted in duplicate at a concentration of 20 mM in 150 mM sodium phosphate (pH 8.5), Arabidopsis oligonucleotide spike controls (Stratagene SpotReport, La Jolla, CA) spotted in quadruplicate, and 10 human and 10 yeast oligonucleotide negative controls spotted in duplicate. The oligonucleotides were printed on Codelink slides (Amersham, Piscataway, NJ) by Microarrays, Inc. (Nashville, TN). Post-print processing was conducted according to the manufacturerÃ¢â‚¬â„¢s recommendations.
spotted DNA/cDNA, coffee nylon arrays, Nylon membranes containing coffee ESTs from subtracted cDNA librares. Plasmid clones were used. A set of sixteen 96-well plates was spotted on two nylon filters (85 mm x 125 mm) in a 4 x 4 array configuration, with a 96 pins hand-held array tool