Gene Expression Omnibus (GEO) Overview Version：2013-04-06Japanese page
An overview of the GEO entries broken down by the measurement platforms and the features of the measured samples.
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Data Unit : [ DataSet / Sample / Platform ] Show explanation>> <<Hide explanation
DataSet : Series(GSE) x Platform(GPL). A set of related gene expression data.
Sample : Biological materials.
platform : Methods or instruments used for the gene expression profilings.
The numbers shown in the tabs are the numbers of the data (series, samples or platforms) belonging to the groups.
  Human
(2,851)		   Primates
(60)		   Rodents
(2,590)		   Mammals
(41)		   Vertebrates
(39)		   Invertebrates
(59)		   Plants
(464)		   Bacteria
(78)		   Viruses
(0)		   Phages
(0)		   Unclassified
(120)		   All
(6,302)		  
  SAGE NlaIII
(5)		   SAGE RsaI
(0)		   SAGE Sau3A
(0)		   MPSS
(0)		   GeneChip
(132)		   Tiling Array
(15)		   cDNA Array
(222)		   Oligo Array
(90)		   Bead Array
(0)		   Protein Array
(0)		   Antibody
(0)		   RT-PCR
(0)		   HT-Seq
(0)		   Other
(0)		   All
(464)		  
  brain
(0)		   blood
(0)		   connective
(0)		   reproductive
(0)		   muscular
(0)		   digestive
(0)		   liver
(0)		   lung
(0)		   urinary
(0)		   endo/exo-crine
(0)		   embryo
(0)		   adult aerial structure
(23)		   young aerial structure
(0)		   root
(0)		   meristem/growing tissue
(12)		   flower/sexual organ
(6)		   seed/fruit/grain
(0)		   pooled
(76)		   unclassified
(347)		   all
(464)		  
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Sample ID Title Number of Data Institute Submission date Platform Sample type Species Organ class Reasoning of the classification
Keywords used for the classification are shown with bold font.
1 GSM34701 YSS89, T=60, c 9,335 University of Geneva 2004-11-05 [GeneChip] [YG_S98] Affymetrix Yeast Genome S98 Array (GPL90) RNA Saccharomyces cerevisiae
Saccharomyces cerevisiae		 unclassified source_name:Yeast title:YSS89, T=60, c description:Experiment Design: •Type of experiment: time course after GAL (galactose) induction •Experimental factor: time, genotype •Number of hybridizations performed in the experiment: 24 (eight conditions with three biological replicates each) Samples used, extract preparation and labeling: •The origin of the biological sample: Species: S. cerevisiae Cell type: wild-type (YSS37, IFH1-13myc-TRP1, MATa) UASIFH1::HIS3-UASGAL1 (YSS89, UASGAL1-IFH1-13myc-TRP1, MATa) •Growth conditions: log phase (2x107/mL) at 30°C in YPLG medium, then addition of Galactose to 2% •Treatments: all cells were harvested by rapid centrifugation at 4°C then flash frozen in liquid nitrogen. •RNA extraction: RNeasy Protect mini kit (Quiagen) •Labeling protocol(s): cDNA synthesis using Invitrogen reagents according to Affymetrix protocol (5 µg of total RNA) GeneChip IVT labeling kit (Affymetrix) •External controls (spikes): according to the Affymetrix protocols Hybridization controls Poly-A spike controls Keywords = IFH1 Keywords = Transcriptional control of RP genes
2 GSM34696 YSS89, T=60, b 9,335 University of Geneva 2004-11-05 [GeneChip] [YG_S98] Affymetrix Yeast Genome S98 Array (GPL90) RNA Saccharomyces cerevisiae
Saccharomyces cerevisiae		 unclassified source_name:Yeast title:YSS89, T=60, b description:Experiment Design: •Type of experiment: time course after GAL (galactose) induction •Experimental factor: time, genotype •Number of hybridizations performed in the experiment: 24 (eight conditions with three biological replicates each) Samples used, extract preparation and labeling: •The origin of the biological sample: Species: S. cerevisiae Cell type: wild-type (YSS37, IFH1-13myc-TRP1, MATa) UASIFH1::HIS3-UASGAL1 (YSS89, UASGAL1-IFH1-13myc-TRP1, MATa) •Growth conditions: log phase (2x107/mL) at 30°C in YPLG medium, then addition of Galactose to 2% •Treatments: all cells were harvested by rapid centrifugation at 4°C then flash frozen in liquid nitrogen. •RNA extraction: RNeasy Protect mini kit (Quiagen) •Labeling protocol(s): cDNA synthesis using Invitrogen reagents according to Affymetrix protocol (5 µg of total RNA) GeneChip IVT labeling kit (Affymetrix) •External controls (spikes): according to the Affymetrix protocols Hybridization controls Poly-A spike controls Keywords = IFH1 Keywords = Transcriptional control of RP genes
3 GSM34691 YSS89, T=60 9,335 University of Geneva 2004-11-05 [GeneChip] [YG_S98] Affymetrix Yeast Genome S98 Array (GPL90) RNA Saccharomyces cerevisiae
Saccharomyces cerevisiae		 unclassified source_name:Yeast title:YSS89, T=60 description:Experiment Design: •Type of experiment: time course after GAL (galactose) induction •Experimental factor: time, genotype •Number of hybridizations performed in the experiment: 24 (eight conditions with three biological replicates each) Samples used, extract preparation and labeling: •The origin of the biological sample: Species: S. cerevisiae Cell type: wild-type (YSS37, IFH1-13myc-TRP1, MATa) UASIFH1::HIS3-UASGAL1 (YSS89, UASGAL1-IFH1-13myc-TRP1, MATa) •Growth conditions: log phase (2x107/mL) at 30°C in YPLG medium, then addition of Galactose to 2% •Treatments: all cells were harvested by rapid centrifugation at 4°C then flash frozen in liquid nitrogen. •RNA extraction: RNeasy Protect mini kit (Quiagen) •Labeling protocol(s): cDNA synthesis using Invitrogen reagents according to Affymetrix protocol (5 µg of total RNA) GeneChip IVT labeling kit (Affymetrix) •External controls (spikes): according to the Affymetrix protocols Hybridization controls Poly-A spike controls Keywords = IFH1 Keywords = Transcriptional control of RP genes
4 GSM34700 YSS89, T=40, c 9,335 University of Geneva 2004-11-05 [GeneChip] [YG_S98] Affymetrix Yeast Genome S98 Array (GPL90) RNA Saccharomyces cerevisiae
Saccharomyces cerevisiae		 unclassified source_name:Yeast title:YSS89, T=40, c description:Experiment Design: •Type of experiment: time course after GAL (galactose) induction •Experimental factor: time, genotype •Number of hybridizations performed in the experiment: 24 (eight conditions with three biological replicates each) Samples used, extract preparation and labeling: •The origin of the biological sample: Species: S. cerevisiae Cell type: wild-type (YSS37, IFH1-13myc-TRP1, MATa) UASIFH1::HIS3-UASGAL1 (YSS89, UASGAL1-IFH1-13myc-TRP1, MATa) •Growth conditions: log phase (2x107/mL) at 30°C in YPLG medium, then addition of Galactose to 2% •Treatments: all cells were harvested by rapid centrifugation at 4°C then flash frozen in liquid nitrogen. •RNA extraction: RNeasy Protect mini kit (Quiagen) •Labeling protocol(s): cDNA synthesis using Invitrogen reagents according to Affymetrix protocol (5 µg of total RNA) GeneChip IVT labeling kit (Affymetrix) •External controls (spikes): according to the Affymetrix protocols Hybridization controls Poly-A spike controls Keywords = IFH1 Keywords = Transcriptional control of RP genes
5 GSM34695 YSS89, T=40, b 9,335 University of Geneva 2004-11-05 [GeneChip] [YG_S98] Affymetrix Yeast Genome S98 Array (GPL90) RNA Saccharomyces cerevisiae
Saccharomyces cerevisiae		 unclassified source_name:Yeast title:YSS89, T=40, b description:Experiment Design: •Type of experiment: time course after GAL (galactose) induction •Experimental factor: time, genotype •Number of hybridizations performed in the experiment: 24 (eight conditions with three biological replicates each) Samples used, extract preparation and labeling: •The origin of the biological sample: Species: S. cerevisiae Cell type: wild-type (YSS37, IFH1-13myc-TRP1, MATa) UASIFH1::HIS3-UASGAL1 (YSS89, UASGAL1-IFH1-13myc-TRP1, MATa) •Growth conditions: log phase (2x107/mL) at 30°C in YPLG medium, then addition of Galactose to 2% •Treatments: all cells were harvested by rapid centrifugation at 4°C then flash frozen in liquid nitrogen. •RNA extraction: RNeasy Protect mini kit (Quiagen) •Labeling protocol(s): cDNA synthesis using Invitrogen reagents according to Affymetrix protocol (5 µg of total RNA) GeneChip IVT labeling kit (Affymetrix) •External controls (spikes): according to the Affymetrix protocols Hybridization controls Poly-A spike controls Keywords = IFH1 Keywords = Transcriptional control of RP genes
6 GSM34690 YSS89, T=40 9,335 University of Geneva 2004-11-05 [GeneChip] [YG_S98] Affymetrix Yeast Genome S98 Array (GPL90) RNA Saccharomyces cerevisiae
Saccharomyces cerevisiae		 unclassified source_name:Yeast title:YSS89, T=40 description:Experiment Design: •Type of experiment: time course after GAL (galactose) induction •Experimental factor: time, genotype •Number of hybridizations performed in the experiment: 24 (eight conditions with three biological replicates each) Samples used, extract preparation and labeling: •The origin of the biological sample: Species: S. cerevisiae Cell type: wild-type (YSS37, IFH1-13myc-TRP1, MATa) UASIFH1::HIS3-UASGAL1 (YSS89, UASGAL1-IFH1-13myc-TRP1, MATa) •Growth conditions: log phase (2x107/mL) at 30°C in YPLG medium, then addition of Galactose to 2% •Treatments: all cells were harvested by rapid centrifugation at 4°C then flash frozen in liquid nitrogen. •RNA extraction: RNeasy Protect mini kit (Quiagen) •Labeling protocol(s): cDNA synthesis using Invitrogen reagents according to Affymetrix protocol (5 µg of total RNA) GeneChip IVT labeling kit (Affymetrix) •External controls (spikes): according to the Affymetrix protocols Hybridization controls Poly-A spike controls Keywords = IFH1 Keywords = Transcriptional control of RP genes
7 GSM34699 YSS89, T=30, c 9,335 University of Geneva 2004-11-05 [GeneChip] [YG_S98] Affymetrix Yeast Genome S98 Array (GPL90) RNA Saccharomyces cerevisiae
Saccharomyces cerevisiae		 unclassified source_name:Yeast title:YSS89, T=30, c description:Experiment Design: •Type of experiment: time course after GAL (galactose) induction •Experimental factor: time, genotype •Number of hybridizations performed in the experiment: 24 (eight conditions with three biological replicates each) Samples used, extract preparation and labeling: •The origin of the biological sample: Species: S. cerevisiae Cell type: wild-type (YSS37, IFH1-13myc-TRP1, MATa) UASIFH1::HIS3-UASGAL1 (YSS89, UASGAL1-IFH1-13myc-TRP1, MATa) •Growth conditions: log phase (2x107/mL) at 30°C in YPLG medium, then addition of Galactose to 2% •Treatments: all cells were harvested by rapid centrifugation at 4°C then flash frozen in liquid nitrogen. •RNA extraction: RNeasy Protect mini kit (Quiagen) •Labeling protocol(s): cDNA synthesis using Invitrogen reagents according to Affymetrix protocol (5 µg of total RNA) GeneChip IVT labeling kit (Affymetrix) •External controls (spikes): according to the Affymetrix protocols Hybridization controls Poly-A spike controls Keywords = IFH1 Keywords = Transcriptional control of RP genes
8 GSM34694 YSS89, T=30, b 9,335 University of Geneva 2004-11-05 [GeneChip] [YG_S98] Affymetrix Yeast Genome S98 Array (GPL90) RNA Saccharomyces cerevisiae
Saccharomyces cerevisiae		 unclassified source_name:Yeast title:YSS89, T=30, b description:Experiment Design: Keywords = IFH1 Keywords = Transcriptional control of RP genes
9 GSM34689 YSS89, T=30 9,335 University of Geneva 2004-11-05 [GeneChip] [YG_S98] Affymetrix Yeast Genome S98 Array (GPL90) RNA Saccharomyces cerevisiae
Saccharomyces cerevisiae		 unclassified source_name:Yeast title:YSS89, T=30 description:Experiment Design: •Type of experiment: time course after GAL (galactose) induction •Experimental factor: time, genotype •Number of hybridizations performed in the experiment: 24 (eight conditions with three biological replicates each) Samples used, extract preparation and labeling: •The origin of the biological sample: Species: S. cerevisiae Cell type: wild-type (YSS37, IFH1-13myc-TRP1, MATa) UASIFH1::HIS3-UASGAL1 (YSS89, UASGAL1-IFH1-13myc-TRP1, MATa) •Growth conditions: log phase (2x107/mL) at 30°C in YPLG medium, then addition of Galactose to 2% •Treatments: all cells were harvested by rapid centrifugation at 4°C then flash frozen in liquid nitrogen. •RNA extraction: RNeasy Protect mini kit (Quiagen) •Labeling protocol(s): cDNA synthesis using Invitrogen reagents according to Affymetrix protocol (5 µg of total RNA) GeneChip IVT labeling kit (Affymetrix) •External controls (spikes): according to the Affymetrix protocols Hybridization controls Poly-A spike controls Keywords = IFH1 Keywords = Transcriptional control of RP genes
10 GSM34698 YSS89, T=20, c 9,335 University of Geneva 2004-11-05 [GeneChip] [YG_S98] Affymetrix Yeast Genome S98 Array (GPL90) RNA Saccharomyces cerevisiae
Saccharomyces cerevisiae		 unclassified source_name:Yeast title:YSS89, T=20, c description:Experiment Design: •Type of experiment: time course after GAL (galactose) induction •Experimental factor: time, genotype •Number of hybridizations performed in the experiment: 24 (eight conditions with three biological replicates each) Samples used, extract preparation and labeling: •The origin of the biological sample: Species: S. cerevisiae Cell type: wild-type (YSS37, IFH1-13myc-TRP1, MATa) UASIFH1::HIS3-UASGAL1 (YSS89, UASGAL1-IFH1-13myc-TRP1, MATa) •Growth conditions: log phase (2x107/mL) at 30°C in YPLG medium, then addition of Galactose to 2% •Treatments: all cells were harvested by rapid centrifugation at 4°C then flash frozen in liquid nitrogen. •RNA extraction: RNeasy Protect mini kit (Quiagen) •Labeling protocol(s): cDNA synthesis using Invitrogen reagents according to Affymetrix protocol (5 µg of total RNA) GeneChip IVT labeling kit (Affymetrix) •External controls (spikes): according to the Affymetrix protocols Hybridization controls Poly-A spike controls Keywords = IFH1 Keywords = Transcriptional control of RP genes
11 GSM34693 YSS89, T=20, b 9,335 University of Geneva 2004-11-05 [GeneChip] [YG_S98] Affymetrix Yeast Genome S98 Array (GPL90) RNA Saccharomyces cerevisiae
Saccharomyces cerevisiae		 unclassified source_name:Yeast title:YSS89, T=20, b description:Experiment Design: •Type of experiment: time course after GAL (galactose) induction •Experimental factor: time, genotype •Number of hybridizations performed in the experiment: 24 (eight conditions with three biological replicates each) Samples used, extract preparation and labeling: •The origin of the biological sample: Species: S. cerevisiae Cell type: wild-type (YSS37, IFH1-13myc-TRP1, MATa) UASIFH1::HIS3-UASGAL1 (YSS89, UASGAL1-IFH1-13myc-TRP1, MATa) •Growth conditions: log phase (2x107/mL) at 30°C in YPLG medium, then addition of Galactose to 2% •Treatments: all cells were harvested by rapid centrifugation at 4°C then flash frozen in liquid nitrogen. •RNA extraction: RNeasy Protect mini kit (Quiagen) •Labeling protocol(s): cDNA synthesis using Invitrogen reagents according to Affymetrix protocol (5 µg of total RNA) GeneChip IVT labeling kit (Affymetrix) •External controls (spikes): according to the Affymetrix protocols Hybridization controls Poly-A spike controls Keywords = IFH1 Keywords = Transcriptional control of RP genes
12 GSM34688 YSS89, T=20 9,335 University of Geneva 2004-11-05 [GeneChip] [YG_S98] Affymetrix Yeast Genome S98 Array (GPL90) RNA Saccharomyces cerevisiae
Saccharomyces cerevisiae		 unclassified source_name:Yeast title:YSS89, T=20 description:Experiment Design: •Type of experiment: time course after GAL (galactose) induction •Experimental factor: time, genotype •Number of hybridizations performed in the experiment: 24 (eight conditions with three biological replicates each) Samples used, extract preparation and labeling: •The origin of the biological sample: Species: S. cerevisiae Cell type: wild-type (YSS37, IFH1-13myc-TRP1, MATa) UASIFH1::HIS3-UASGAL1 (YSS89, UASGAL1-IFH1-13myc-TRP1, MATa) •Growth conditions: log phase (2x107/mL) at 30°C in YPLG medium, then addition of Galactose to 2% •Treatments: all cells were harvested by rapid centrifugation at 4°C then flash frozen in liquid nitrogen. •RNA extraction: RNeasy Protect mini kit (Quiagen) •Labeling protocol(s): cDNA synthesis using Invitrogen reagents according to Affymetrix protocol (5 µg of total RNA) GeneChip IVT labeling kit (Affymetrix) •External controls (spikes): according to the Affymetrix protocols Hybridization controls Poly-A spike controls Keywords = IFH1 Keywords = Transcriptional control of RP genes
13 GSM34697 YSS89, T=0, c 9,335 University of Geneva 2004-11-05 [GeneChip] [YG_S98] Affymetrix Yeast Genome S98 Array (GPL90) RNA Saccharomyces cerevisiae
Saccharomyces cerevisiae		 unclassified source_name:Yeast title:YSS89, T=0, c description:Experiment Design: •Type of experiment: time course after GAL (galactose) induction •Experimental factor: time, genotype •Number of hybridizations performed in the experiment: 24 (eight conditions with three biological replicates each) Samples used, extract preparation and labeling: •The origin of the biological sample: Species: S. cerevisiae Cell type: wild-type (YSS37, IFH1-13myc-TRP1, MATa) UASIFH1::HIS3-UASGAL1 (YSS89, UASGAL1-IFH1-13myc-TRP1, MATa) •Growth conditions: log phase (2x107/mL) at 30°C in YPLG medium, then addition of Galactose to 2% •Treatments: all cells were harvested by rapid centrifugation at 4°C then flash frozen in liquid nitrogen. •RNA extraction: RNeasy Protect mini kit (Quiagen) •Labeling protocol(s): cDNA synthesis using Invitrogen reagents according to Affymetrix protocol (5 µg of total RNA) GeneChip IVT labeling kit (Affymetrix) •External controls (spikes): according to the Affymetrix protocols Hybridization controls Poly-A spike controls Keywords = IFH1 Keywords = Transcriptional control of RP genes
14 GSM34692 YSS89, T=0, b 9,335 University of Geneva 2004-11-05 [GeneChip] [YG_S98] Affymetrix Yeast Genome S98 Array (GPL90) RNA Saccharomyces cerevisiae
Saccharomyces cerevisiae		 unclassified source_name:Yeast title:YSS89, T=0, b description:Experiment Design: •Type of experiment: time course after GAL (galactose) induction •Experimental factor: time, genotype •Number of hybridizations performed in the experiment: 24 (eight conditions with three biological replicates each) Samples used, extract preparation and labeling: •The origin of the biological sample: Species: S. cerevisiae Cell type: wild-type (YSS37, IFH1-13myc-TRP1, MATa) UASIFH1::HIS3-UASGAL1 (YSS89, UASGAL1-IFH1-13myc-TRP1, MATa) •Growth conditions: log phase (2x107/mL) at 30°C in YPLG medium, then addition of Galactose to 2% •Treatments: all cells were harvested by rapid centrifugation at 4°C then flash frozen in liquid nitrogen. •RNA extraction: RNeasy Protect mini kit (Quiagen) •Labeling protocol(s): cDNA synthesis using Invitrogen reagents according to Affymetrix protocol (5 µg of total RNA) GeneChip IVT labeling kit (Affymetrix) •External controls (spikes): according to the Affymetrix protocols Hybridization controls Poly-A spike controls Keywords = IFH1 Keywords = Transcriptional control of RP genes
15 GSM34687 YSS89, T=0 9,335 University of Geneva 2004-11-05 [GeneChip] [YG_S98] Affymetrix Yeast Genome S98 Array (GPL90) RNA Saccharomyces cerevisiae
Saccharomyces cerevisiae		 unclassified source_name:Yeast title:YSS89, T=0 description:Experiment Design: •Type of experiment: time course after GAL (galactose) induction •Experimental factor: time, genotype •Number of hybridizations performed in the experiment: 24 (eight conditions with three biological replicates each) Samples used, extract preparation and labeling: •The origin of the biological sample: Species: S. cerevisiae Cell type: wild-type (YSS37, IFH1-13myc-TRP1, MATa) UASIFH1::HIS3-UASGAL1 (YSS89, UASGAL1-IFH1-13myc-TRP1, MATa) •Growth conditions: log phase (2x107/mL) at 30°C in YPLG medium, then addition of Galactose to 2% •Treatments: all cells were harvested by rapid centrifugation at 4°C then flash frozen in liquid nitrogen. •RNA extraction: RNeasy Protect mini kit (Quiagen) •Labeling protocol(s): cDNA synthesis using Invitrogen reagents according to Affymetrix protocol (5 µg of total RNA) GeneChip IVT labeling kit (Affymetrix) •External controls (spikes): according to the Affymetrix protocols Hybridization controls Poly-A spike controls Keywords = IFH1 Keywords = Transcriptional control of RP genes
16 GSM34686 YSS37, T=60, c 9,335 University of Geneva 2004-11-05 [GeneChip] [YG_S98] Affymetrix Yeast Genome S98 Array (GPL90) RNA Saccharomyces cerevisiae
Saccharomyces cerevisiae		 unclassified source_name:Yeast title:YSS37, T=60, c description:Experiment Design: •Type of experiment: time course after GAL (galactose) induction •Experimental factor: time, genotype •Number of hybridizations performed in the experiment: 24 (eight conditions with three biological replicates each) Samples used, extract preparation and labeling: •The origin of the biological sample: Species: S. cerevisiae Cell type: wild-type (YSS37, IFH1-13myc-TRP1, MATa) UASIFH1::HIS3-UASGAL1 (YSS89, UASGAL1-IFH1-13myc-TRP1, MATa) •Growth conditions: log phase (2x107/mL) at 30°C in YPLG medium, then addition of Galactose to 2% •Treatments: all cells were harvested by rapid centrifugation at 4°C then flash frozen in liquid nitrogen. •RNA extraction: RNeasy Protect mini kit (Quiagen) •Labeling protocol(s): cDNA synthesis using Invitrogen reagents according to Affymetrix protocol (5 µg of total RNA) GeneChip IVT labeling kit (Affymetrix) •External controls (spikes): according to the Affymetrix protocols Hybridization controls Poly-A spike controls Keywords = IFH1 Keywords = Transcriptional control of RP genes
17 GSM34683 YSS37, T=60, b 9,335 University of Geneva 2004-11-05 [GeneChip] [YG_S98] Affymetrix Yeast Genome S98 Array (GPL90) RNA Saccharomyces cerevisiae
Saccharomyces cerevisiae		 unclassified source_name:Yeast title:YSS37, T=60, b description:Experiment Design: •Type of experiment: time course after GAL (galactose) induction •Experimental factor: time, genotype •Number of hybridizations performed in the experiment: 24 (eight conditions with three biological replicates each) Samples used, extract preparation and labeling: •The origin of the biological sample: Species: S. cerevisiae Cell type: wild-type (YSS37, IFH1-13myc-TRP1, MATa) UASIFH1::HIS3-UASGAL1 (YSS89, UASGAL1-IFH1-13myc-TRP1, MATa) •Growth conditions: log phase (2x107/mL) at 30°C in YPLG medium, then addition of Galactose to 2% •Treatments: all cells were harvested by rapid centrifugation at 4°C then flash frozen in liquid nitrogen. •RNA extraction: RNeasy Protect mini kit (Quiagen) •Labeling protocol(s): cDNA synthesis using Invitrogen reagents according to Affymetrix protocol (5 µg of total RNA) GeneChip IVT labeling kit (Affymetrix) •External controls (spikes): according to the Affymetrix protocols Hybridization controls Poly-A spike controls Keywords = IFH1 Keywords = Transcriptional control of RP genes
18 GSM34680 YSS37, T=60 9,335 University of Geneva 2004-11-05 [GeneChip] [YG_S98] Affymetrix Yeast Genome S98 Array (GPL90) RNA Saccharomyces cerevisiae
Saccharomyces cerevisiae		 unclassified source_name:Yeast title:YSS37, T=60 description:Experiment Design: •Type of experiment: time course after GAL (galactose) induction •Experimental factor: time, genotype •Number of hybridizations performed in the experiment: 24 (eight conditions with three biological replicates each) Samples used, extract preparation and labeling: •The origin of the biological sample: Species: S. cerevisiae Cell type: wild-type (YSS37, IFH1-13myc-TRP1, MATa) UASIFH1::HIS3-UASGAL1 (YSS89, UASGAL1-IFH1-13myc-TRP1, MATa) •Growth conditions: log phase (2x107/mL) at 30°C in YPLG medium, then addition of Galactose to 2% •Treatments: all cells were harvested by rapid centrifugation at 4°C then flash frozen in liquid nitrogen. •RNA extraction: RNeasy Protect mini kit (Quiagen) •Labeling protocol(s): cDNA synthesis using Invitrogen reagents according to Affymetrix protocol (5 µg of total RNA) GeneChip IVT labeling kit (Affymetrix) •External controls (spikes): according to the Affymetrix protocols Hybridization controls Poly-A spike controls Keywords = IFH1 Keywords = Transcriptional control of RP genes
19 GSM34685 YSS37, T=30, c 9,335 University of Geneva 2004-11-05 [GeneChip] [YG_S98] Affymetrix Yeast Genome S98 Array (GPL90) RNA Saccharomyces cerevisiae
Saccharomyces cerevisiae		 unclassified source_name:Yeast title:YSS37, T=30, c description:Experiment Design: •Type of experiment: time course after GAL (galactose) induction •Experimental factor: time, genotype •Number of hybridizations performed in the experiment: 24 (eight conditions with three biological replicates each) Samples used, extract preparation and labeling: •The origin of the biological sample: Species: S. cerevisiae Cell type: wild-type (YSS37, IFH1-13myc-TRP1, MATa) UASIFH1::HIS3-UASGAL1 (YSS89, UASGAL1-IFH1-13myc-TRP1, MATa) •Growth conditions: log phase (2x107/mL) at 30°C in YPLG medium, then addition of Galactose to 2% •Treatments: all cells were harvested by rapid centrifugation at 4°C then flash frozen in liquid nitrogen. •RNA extraction: RNeasy Protect mini kit (Quiagen) •Labeling protocol(s): cDNA synthesis using Invitrogen reagents according to Affymetrix protocol (5 µg of total RNA) GeneChip IVT labeling kit (Affymetrix) •External controls (spikes): according to the Affymetrix protocols Hybridization controls Poly-A spike controls Keywords = IFH1 Keywords = Transcriptional control of RP genes
20 GSM34682 YSS37, T=30, b 9,335 University of Geneva 2004-11-05 [GeneChip] [YG_S98] Affymetrix Yeast Genome S98 Array (GPL90) RNA Saccharomyces cerevisiae
Saccharomyces cerevisiae		 unclassified source_name:Yeast title:YSS37, T=30, b description:Experiment Design: Keywords = IFH1 Keywords = Transcriptional control of RP genes
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