Gene Expression Omnibus (GEO) Overview Version:2013-04-06Japanese page
An overview of the GEO entries broken down by the measurement platforms and the features of the measured samples.
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Data Unit : [ DataSet / Sample / Platform ] Show explanation>> <<Hide explanation
DataSet : Series(GSE) x Platform(GPL). A set of related gene expression data.
Sample : Biological materials.
platform : Methods or instruments used for the gene expression profilings.
The numbers shown in the tabs are the numbers of the data (series, samples or platforms) belonging to the groups.
  Human
(4,712)
  Primates
(41)
  Rodents
(1,963)
  Mammals
(85)
  Vertebrates
(71)
  Invertebrates
(162)
  Plants
(837)
  Bacteria
(313)
  Viruses
(0)
  Phages
(18)
  Unclassified
(320)
  All
(8,522)
 
  SAGE NlaIII
(2)
  SAGE RsaI
(0)
  SAGE Sau3A
(0)
  MPSS
(0)
  GeneChip
(137)
  Tiling Array
(8)
  cDNA Array
(613)
  Oligo Array
(45)
  Bead Array
(0)
  Protein Array
(0)
  Antibody
(0)
  RT-PCR
(0)
  HT-Seq
(0)
  Other
(2)
  All
(837)
 
  brain
(16)
  blood
(0)
  connective
(0)
  reproductive
(0)
  muscular
(0)
  digestive
(0)
  liver
(0)
  lung
(0)
  urinary
(0)
  endo/exo-crine
(0)
  embryo
(0)
  adult aerial structure
(63)
  young aerial structure
(22)
  root
(40)
  meristem/growing tissue
(21)
  flower/sexual organ
(144)
  seed/fruit/grain
(0)
  pooled
(39)
  unclassified
(492)
  all
(837)
 
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Sample ID Title Number of Data Institute Submission date Platform Sample type Species Organ class Reasoning of the classification
Keywords used for the classification are shown with bold font.
1 GSM51632 WT_vs_SIPK-Ri_b_cont_1 32,448 The Institute for Genomic Research 2005-05-17 [cDNA Array] Potato 10k cDNA array version 2 (GPL1901) RNA Nicotiana tabacum
Nicotiana tabacum
unclassified source_name:Query sample; Genotype: Xanthi SIPK-Ri; Tissue: Cell suspension culture; Treatment: Biotic control, 0 h; CH2: Cy3 Reference sample; Genotype: Xanthi; Tissue: Cell suspension culture; Treatment: Biotic control, 0 h; CH1: Cy5 title:WT_vs_SIPK-Ri_b_cont_1 description:Wildtype and SIPK -silenced (RNAi) tobacco cell suspension cultures were grown in MS medium at 25 C in the dark. Cell suspension cultures were transferred to fresh growth medium 3 days prior to pooling each genotype and dividing the pool into 3 x 10ml volumes per genotype. A 30 minute pre-incubation was used with xanthine (for abiotic controls and treatments), but no pre-treatment was used for the biotic stressors. For each treatment and abiotic/biotic controls, the cells were harvested by vacuum filtration at the appropriate time points (controls sampled at 0 hrs) and frozen in N2(l) for RNA extraction. In order to discern the effect of silencing SIPK on the transciptome in defence response, biological replicates of WT and SIPK-Ri were randomly paired on 3 slides per treatment or control group. Keywords = Nicotiana tabacum, Cell suspension culture, Biotic stress
2 GSM51633 WT_vs_SIPK-Ri_b_cont_2 32,448 The Institute for Genomic Research 2005-05-17 [cDNA Array] Potato 10k cDNA array version 2 (GPL1901) RNA Nicotiana tabacum
Nicotiana tabacum
unclassified source_name:Query sample; Genotype: Xanthi SIPK-Ri; Tissue: Cell suspension culture; Treatment: Biotic control, 0 h; CH2: Cy3 Reference sample; Genotype: Xanthi; Tissue: Cell suspension culture; Treatment: Biotic control, 0 h; CH1: Cy5 title:WT_vs_SIPK-Ri_b_cont_2 description:Wildtype and SIPK -silenced (RNAi) tobacco cell suspension cultures were grown in MS medium at 25 C in the dark. Cell suspension cultures were transferred to fresh growth medium 3 days prior to pooling each genotype and dividing the pool into 3 x 10ml volumes per genotype. A 30 minute pre-incubation was used with xanthine (for abiotic controls and treatments), but no pre-treatment was used for the biotic stressors. For each treatment and abiotic/biotic controls, the cells were harvested by vacuum filtration at the appropriate time points (controls sampled at 0 hrs) and frozen in N2(l) for RNA extraction. In order to discern the effect of silencing SIPK on the transciptome in defence response, biological replicates of WT and SIPK-Ri were randomly paired on 3 slides per treatment or control group. Keywords = Nicotiana tabacum, Cell suspension culture, Biotic stress
3 GSM51634 WT_vs_SIPK-Ri_b_cont_3 32,448 The Institute for Genomic Research 2005-05-17 [cDNA Array] Potato 10k cDNA array version 2 (GPL1901) RNA Nicotiana tabacum
Nicotiana tabacum
unclassified source_name:Query sample; Genotype: Xanthi SIPK-Ri; Tissue: Cell suspension culture; Treatment: Biotic control, 0 h; CH2: Cy3 Reference sample; Genotype: Xanthi; Tissue: Cell suspension culture; Treatment: Biotic control, 0 h; CH1: Cy5 title:WT_vs_SIPK-Ri_b_cont_3 description:Wildtype and SIPK -silenced (RNAi) tobacco cell suspension cultures were grown in MS medium at 25 C in the dark. Cell suspension cultures were transferred to fresh growth medium 3 days prior to pooling each genotype and dividing the pool into 3 x 10ml volumes per genotype. A 30 minute pre-incubation was used with xanthine (for abiotic controls and treatments), but no pre-treatment was used for the biotic stressors. For each treatment and abiotic/biotic controls, the cells were harvested by vacuum filtration at the appropriate time points (controls sampled at 0 hrs) and frozen in N2(l) for RNA extraction. In order to discern the effect of silencing SIPK on the transciptome in defence response, biological replicates of WT and SIPK-Ri were randomly paired on 3 slides per treatment or control group. Keywords = Nicotiana tabacum, Cell suspension culture, Biotic stress
4 GSM51635 WT_vs_SIPK-Ri_4h_harp_1 32,448 The Institute for Genomic Research 2005-05-17 [cDNA Array] Potato 10k cDNA array version 2 (GPL1901) RNA Nicotiana tabacum
Nicotiana tabacum
unclassified source_name:Query sample; Genotype: Xanthi SIPK-Ri; Tissue: Cell suspension culture; Treatment: Harpin (P.syringae), 4 h; CH2: Cy3 Reference sample; Genotype: Xanthi; Tissue: Cell suspension culture; Treatment: Harpin (P.syringae), 4 h; CH1: Cy5 title:WT_vs_SIPK-Ri_4h_harp_1 description:Wildtype and SIPK -silenced (RNAi) tobacco cell suspension cultures were grown in MS medium at 25 C in the dark. Cell suspension cultures were transferred to fresh growth medium 3 days prior to pooling each genotype and dividing the pool into 3 x 10ml volumes per genotype. A 30 minute pre-incubation was used with xanthine (for abiotic controls and treatments), but no pre-treatment was used for the biotic stressors. For each treatment and abiotic/biotic controls, the cells were harvested by vacuum filtration at the appropriate time points (controls sampled at 0 hrs) and frozen in N2(l) for RNA extraction. In order to discern the effect of silencing SIPK on the transciptome in defence response, biological replicates of WT and SIPK-Ri were randomly paired on 3 slides per treatment or control group. Keywords = Nicotiana tabacum, Cell suspension culture, Biotic stress
5 GSM51636 WT_vs_SIPK-Ri_4h_harp_2 32,448 The Institute for Genomic Research 2005-05-17 [cDNA Array] Potato 10k cDNA array version 2 (GPL1901) RNA Nicotiana tabacum
Nicotiana tabacum
unclassified source_name:Query sample; Genotype: Xanthi SIPK-Ri; Tissue: Cell suspension culture; Treatment: Harpin (P.syringae), 4 h; CH2: Cy3 Reference sample; Genotype: Xanthi; Tissue: Cell suspension culture; Treatment: Harpin (P.syringae), 4 h; CH1: Cy5 title:WT_vs_SIPK-Ri_4h_harp_2 description:Wildtype and SIPK -silenced (RNAi) tobacco cell suspension cultures were grown in MS medium at 25 C in the dark. Cell suspension cultures were transferred to fresh growth medium 3 days prior to pooling each genotype and dividing the pool into 3 x 10ml volumes per genotype. A 30 minute pre-incubation was used with xanthine (for abiotic controls and treatments), but no pre-treatment was used for the biotic stressors. For each treatment and abiotic/biotic controls, the cells were harvested by vacuum filtration at the appropriate time points (controls sampled at 0 hrs) and frozen in N2(l) for RNA extraction. In order to discern the effect of silencing SIPK on the transciptome in defence response, biological replicates of WT and SIPK-Ri were randomly paired on 3 slides per treatment or control group. Keywords = Nicotiana tabacum, Cell suspension culture, Biotic stress
6 GSM51637 WT_vs_SIPK-Ri_4h_harp_3 32,448 The Institute for Genomic Research 2005-05-17 [cDNA Array] Potato 10k cDNA array version 2 (GPL1901) RNA Nicotiana tabacum
Nicotiana tabacum
unclassified source_name:Query sample; Genotype: Xanthi SIPK-Ri; Tissue: Cell suspension culture; Treatment: Harpin (P.syringae), 4 h; CH2: Cy3 Reference sample; Genotype: Xanthi; Tissue: Cell suspension culture; Treatment: Harpin (P.syringae), 4 h; CH1: Cy5 title:WT_vs_SIPK-Ri_4h_harp_3 description:Wildtype and SIPK -silenced (RNAi) tobacco cell suspension cultures were grown in MS medium at 25 C in the dark. Cell suspension cultures were transferred to fresh growth medium 3 days prior to pooling each genotype and dividing the pool into 3 x 10ml volumes per genotype. A 30 minute pre-incubation was used with xanthine (for abiotic controls and treatments), but no pre-treatment was used for the biotic stressors. For each treatment and abiotic/biotic controls, the cells were harvested by vacuum filtration at the appropriate time points (controls sampled at 0 hrs) and frozen in N2(l) for RNA extraction. In order to discern the effect of silencing SIPK on the transciptome in defence response, biological replicates of WT and SIPK-Ri were randomly paired on 3 slides per treatment or control group. Keywords = Nicotiana tabacum, Cell suspension culture, Biotic stress
7 GSM51638 WT_vs_SIPK-Ri_4h_mega_1 32,448 The Institute for Genomic Research 2005-05-17 [cDNA Array] Potato 10k cDNA array version 2 (GPL1901) RNA Nicotiana tabacum
Nicotiana tabacum
unclassified source_name:Query sample; Genotype: Xanthi SIPK-Ri; Tissue: Cell suspension culture; Treatment: Megaspermin (P.megasperma), 4 h; CH2: Cy3 Reference sample; Genotype: Xanthi; Tissue: Cell suspension culture; Treatment: Megaspermin (P.megasperma), 4 h; CH1: Cy5 title:WT_vs_SIPK-Ri_4h_mega_1 description:Wildtype and SIPK -silenced (RNAi) tobacco cell suspension cultures were grown in MS medium at 25 C in the dark. Cell suspension cultures were transferred to fresh growth medium 3 days prior to pooling each genotype and dividing the pool into 3 x 10ml volumes per genotype. A 30 minute pre-incubation was used with xanthine (for abiotic controls and treatments), but no pre-treatment was used for the biotic stressors. For each treatment and abiotic/biotic controls, the cells were harvested by vacuum filtration at the appropriate time points (controls sampled at 0 hrs) and frozen in N2(l) for RNA extraction. In order to discern the effect of silencing SIPK on the transciptome in defence response, biological replicates of WT and SIPK-Ri were randomly paired on 3 slides per treatment or control group. Keywords = Nicotiana tabacum, Cell suspension culture, Biotic stress
8 GSM51639 WT_vs_SIPK-Ri_4h_mega_2 32,448 The Institute for Genomic Research 2005-05-17 [cDNA Array] Potato 10k cDNA array version 2 (GPL1901) RNA Nicotiana tabacum
Nicotiana tabacum
unclassified source_name:Query sample; Genotype: Xanthi SIPK-Ri; Tissue: Cell suspension culture; Treatment: Megaspermin (P.megasperma), 4 h; CH2: Cy3 Reference sample; Genotype: Xanthi; Tissue: Cell suspension culture; Treatment: Megaspermin (P.megasperma), 4 h; CH1: Cy5 title:WT_vs_SIPK-Ri_4h_mega_2 description:Wildtype and SIPK -silenced (RNAi) tobacco cell suspension cultures were grown in MS medium at 25 C in the dark. Cell suspension cultures were transferred to fresh growth medium 3 days prior to pooling each genotype and dividing the pool into 3 x 10ml volumes per genotype. A 30 minute pre-incubation was used with xanthine (for abiotic controls and treatments), but no pre-treatment was used for the biotic stressors. For each treatment and abiotic/biotic controls, the cells were harvested by vacuum filtration at the appropriate time points (controls sampled at 0 hrs) and frozen in N2(l) for RNA extraction. In order to discern the effect of silencing SIPK on the transciptome in defence response, biological replicates of WT and SIPK-Ri were randomly paired on 3 slides per treatment or control group. Keywords = Nicotiana tabacum, Cell suspension culture, Biotic stress
9 GSM51640 WT_vs_SIPK-Ri_4h_mega_3 32,448 The Institute for Genomic Research 2005-05-17 [cDNA Array] Potato 10k cDNA array version 2 (GPL1901) RNA Nicotiana tabacum
Nicotiana tabacum
unclassified source_name:Query sample; Genotype: Xanthi SIPK-Ri; Tissue: Cell suspension culture; Treatment: Megaspermin (P.megasperma), 4 h; CH2: Cy3 Reference sample; Genotype: Xanthi; Tissue: Cell suspension culture; Treatment: Megaspermin (P.megasperma), 4 h; CH1: Cy5 title:WT_vs_SIPK-Ri_4h_mega_3 description:Wildtype and SIPK -silenced (RNAi) tobacco cell suspension cultures were grown in MS medium at 25 C in the dark. Cell suspension cultures were transferred to fresh growth medium 3 days prior to pooling each genotype and dividing the pool into 3 x 10ml volumes per genotype. A 30 minute pre-incubation was used with xanthine (for abiotic controls and treatments), but no pre-treatment was used for the biotic stressors. For each treatment and abiotic/biotic controls, the cells were harvested by vacuum filtration at the appropriate time points (controls sampled at 0 hrs) and frozen in N2(l) for RNA extraction. In order to discern the effect of silencing SIPK on the transciptome in defence response, biological replicates of WT and SIPK-Ri were randomly paired on 3 slides per treatment or control group. Keywords = Nicotiana tabacum, Cell suspension culture, Biotic stress
10 GSM51641 WT_vs_SIPK-Ri_8h_harp_1 32,448 The Institute for Genomic Research 2005-05-17 [cDNA Array] Potato 10k cDNA array version 2 (GPL1901) RNA Nicotiana tabacum
Nicotiana tabacum
unclassified source_name:Query sample; Genotype: Xanthi SIPK-Ri; Tissue: Cell suspension culture; Treatment: Harpin (P.syringae), 8 h; CH2: Cy3 Reference sample; Genotype: Xanthi; Tissue: Cell suspension culture; Treatment: Harpin (P.syringae), 8 h; CH1: Cy5 title:WT_vs_SIPK-Ri_8h_harp_1 description:Wildtype and SIPK -silenced (RNAi) tobacco cell suspension cultures were grown in MS medium at 25 C in the dark. Cell suspension cultures were transferred to fresh growth medium 3 days prior to pooling each genotype and dividing the pool into 3 x 10ml volumes per genotype. A 30 minute pre-incubation was used with xanthine (for abiotic controls and treatments), but no pre-treatment was used for the biotic stressors. For each treatment and abiotic/biotic controls, the cells were harvested by vacuum filtration at the appropriate time points (controls sampled at 0 hrs) and frozen in N2(l) for RNA extraction. In order to discern the effect of silencing SIPK on the transciptome in defence response, biological replicates of WT and SIPK-Ri were randomly paired on 3 slides per treatment or control group. Keywords = Nicotiana tabacum, Cell suspension culture, Biotic stress
11 GSM51642 WT_vs_SIPK-Ri_8h_harp_2 32,448 The Institute for Genomic Research 2005-05-17 [cDNA Array] Potato 10k cDNA array version 2 (GPL1901) RNA Nicotiana tabacum
Nicotiana tabacum
unclassified source_name:Query sample; Genotype: Xanthi SIPK-Ri; Tissue: Cell suspension culture; Treatment: Harpin (P.syringae), 8 h; CH2: Cy3 Reference sample; Genotype: Xanthi; Tissue: Cell suspension culture; Treatment: Harpin (P.syringae), 8 h; CH1: Cy5 title:WT_vs_SIPK-Ri_8h_harp_2 description:Wildtype and SIPK -silenced (RNAi) tobacco cell suspension cultures were grown in MS medium at 25 C in the dark. Cell suspension cultures were transferred to fresh growth medium 3 days prior to pooling each genotype and dividing the pool into 3 x 10ml volumes per genotype. A 30 minute pre-incubation was used with xanthine (for abiotic controls and treatments), but no pre-treatment was used for the biotic stressors. For each treatment and abiotic/biotic controls, the cells were harvested by vacuum filtration at the appropriate time points (controls sampled at 0 hrs) and frozen in N2(l) for RNA extraction. In order to discern the effect of silencing SIPK on the transciptome in defence response, biological replicates of WT and SIPK-Ri were randomly paired on 3 slides per treatment or control group. Keywords = Nicotiana tabacum, Cell suspension culture, Biotic stress
12 GSM51643 WT_vs_SIPK-Ri_8h_harp_3 32,448 The Institute for Genomic Research 2005-05-17 [cDNA Array] Potato 10k cDNA array version 2 (GPL1901) RNA Nicotiana tabacum
Nicotiana tabacum
unclassified source_name:Query sample; Genotype: Xanthi SIPK-Ri; Tissue: Cell suspension culture; Treatment: Harpin (P.syringae), 8 h; CH2: Cy3 Reference sample; Genotype: Xanthi; Tissue: Cell suspension culture; Treatment: Harpin (P.syringae), 8 h; CH1: Cy5 title:WT_vs_SIPK-Ri_8h_harp_3 description:Wildtype and SIPK -silenced (RNAi) tobacco cell suspension cultures were grown in MS medium at 25 C in the dark. Cell suspension cultures were transferred to fresh growth medium 3 days prior to pooling each genotype and dividing the pool into 3 x 10ml volumes per genotype. A 30 minute pre-incubation was used with xanthine (for abiotic controls and treatments), but no pre-treatment was used for the biotic stressors. For each treatment and abiotic/biotic controls, the cells were harvested by vacuum filtration at the appropriate time points (controls sampled at 0 hrs) and frozen in N2(l) for RNA extraction. In order to discern the effect of silencing SIPK on the transciptome in defence response, biological replicates of WT and SIPK-Ri were randomly paired on 3 slides per treatment or control group. Keywords = Nicotiana tabacum, Cell suspension culture, Biotic stress
13 GSM51644 WT_vs_SIPK-Ri_8h_mega_1 32,448 The Institute for Genomic Research 2005-05-17 [cDNA Array] Potato 10k cDNA array version 2 (GPL1901) RNA Nicotiana tabacum
Nicotiana tabacum
unclassified source_name:Query sample; Genotype: Xanthi SIPK-Ri; Tissue: Cell suspension culture; Treatment: Megaspermin (P.megasperma), 8 h; CH2: Cy3 Reference sample; Genotype: Xanthi; Tissue: Cell suspension culture; Treatment: Megaspermin (P.megasperma), 8 h; CH1: Cy5 title:WT_vs_SIPK-Ri_8h_mega_1 description:Wildtype and SIPK -silenced (RNAi) tobacco cell suspension cultures were grown in MS medium at 25 C in the dark. Cell suspension cultures were transferred to fresh growth medium 3 days prior to pooling each genotype and dividing the pool into 3 x 10ml volumes per genotype. A 30 minute pre-incubation was used with xanthine (for abiotic controls and treatments), but no pre-treatment was used for the biotic stressors. For each treatment and abiotic/biotic controls, the cells were harvested by vacuum filtration at the appropriate time points (controls sampled at 0 hrs) and frozen in N2(l) for RNA extraction. In order to discern the effect of silencing SIPK on the transciptome in defence response, biological replicates of WT and SIPK-Ri were randomly paired on 3 slides per treatment or control group. Keywords = Nicotiana tabacum, Cell suspension culture, Biotic stress
14 GSM51645 WT_vs_SIPK-Ri_8h_mega_2 32,448 The Institute for Genomic Research 2005-05-17 [cDNA Array] Potato 10k cDNA array version 2 (GPL1901) RNA Nicotiana tabacum
Nicotiana tabacum
unclassified source_name:Query sample; Genotype: Xanthi SIPK-Ri; Tissue: Cell suspension culture; Treatment: Megaspermin (P.megasperma), 8 h; CH2: Cy3 Reference sample; Genotype: Xanthi; Tissue: Cell suspension culture; Treatment: Megaspermin (P.megasperma), 8 h; CH1: Cy5 title:WT_vs_SIPK-Ri_8h_mega_2 description:Wildtype and SIPK -silenced (RNAi) tobacco cell suspension cultures were grown in MS medium at 25 C in the dark. Cell suspension cultures were transferred to fresh growth medium 3 days prior to pooling each genotype and dividing the pool into 3 x 10ml volumes per genotype. A 30 minute pre-incubation was used with xanthine (for abiotic controls and treatments), but no pre-treatment was used for the biotic stressors. For each treatment and abiotic/biotic controls, the cells were harvested by vacuum filtration at the appropriate time points (controls sampled at 0 hrs) and frozen in N2(l) for RNA extraction. In order to discern the effect of silencing SIPK on the transciptome in defence response, biological replicates of WT and SIPK-Ri were randomly paired on 3 slides per treatment or control group. Keywords = Nicotiana tabacum, Cell suspension culture, Biotic stress
15 GSM51646 WT_vs_SIPK-Ri_8h_mega_3 32,448 The Institute for Genomic Research 2005-05-17 [cDNA Array] Potato 10k cDNA array version 2 (GPL1901) RNA Nicotiana tabacum
Nicotiana tabacum
unclassified source_name:Query sample; Genotype: Xanthi SIPK-Ri; Tissue: Cell suspension culture; Treatment: Megaspermin (P.megasperma), 8 h; CH2: Cy3 Reference sample; Genotype: Xanthi; Tissue: Cell suspension culture; Treatment: Megaspermin (P.megasperma), 8 h; CH1: Cy5 title:WT_vs_SIPK-Ri_8h_mega_3 description:Wildtype and SIPK -silenced (RNAi) tobacco cell suspension cultures were grown in MS medium at 25 C in the dark. Cell suspension cultures were transferred to fresh growth medium 3 days prior to pooling each genotype and dividing the pool into 3 x 10ml volumes per genotype. A 30 minute pre-incubation was used with xanthine (for abiotic controls and treatments), but no pre-treatment was used for the biotic stressors. For each treatment and abiotic/biotic controls, the cells were harvested by vacuum filtration at the appropriate time points (controls sampled at 0 hrs) and frozen in N2(l) for RNA extraction. In order to discern the effect of silencing SIPK on the transciptome in defence response, biological replicates of WT and SIPK-Ri were randomly paired on 3 slides per treatment or control group. Keywords = Nicotiana tabacum, Cell suspension culture, Biotic stress
16 GSM51647 WT_vs_SIPK-Ri_ab_cont_1 32,448 The Institute for Genomic Research 2005-05-17 [cDNA Array] Potato 10k cDNA array version 2 (GPL1901) RNA Nicotiana tabacum
Nicotiana tabacum
unclassified source_name:Query sample; Genotype: Xanthi SIPK-Ri; Tissue: Cell suspension culture; Treatment: Abiotic biological control, 0 h; CH2: Cy3 Reference sample; Genotype: Xanthi; Tissue: Cell suspension culture; Treatment: Abiotic biological control, 0 h; CH1: Cy5 title:WT_vs_SIPK-Ri_ab_cont_1 description:Wildtype and SIPK -silenced (RNAi) tobacco cell suspension cultures were grown in MS medium at 25 C in the dark. Cell suspension cultures were transferred to fresh growth medium 3 days prior to pooling each genotype and dividing the pool into 3 x 10ml volumes per genotype. A 30 minute pre-incubation was used with xanthine (for abiotic controls and treatments), but no pre-treatment was used for the biotic stressors. For each treatment and abiotic/biotic controls, the cells were harvested by vacuum filtration at the appropriate time points (controls sampled at 0 hrs) and frozen in N2(l) for RNA extraction. In order to discern the effect of silencing SIPK on the transciptome in defence response, biological replicates of WT and SIPK-Ri were randomly paired on 3 slides per treatment or control group. Keywords = Nicotiana tabacum, Cell suspension culture, Biotic stress
17 GSM51648 WT_vs_SIPK-Ri_ab_cont_2 32,448 The Institute for Genomic Research 2005-05-17 [cDNA Array] Potato 10k cDNA array version 2 (GPL1901) RNA Nicotiana tabacum
Nicotiana tabacum
unclassified source_name:Query sample; Genotype: Xanthi SIPK-Ri; Tissue: Cell suspension culture; Treatment: Abiotic biological control, 0 h; CH2: Cy3 Reference sample; Genotype: Xanthi; Tissue: Cell suspension culture; Treatment: Abiotic biological control, 0 h; CH1: Cy5 title:WT_vs_SIPK-Ri_ab_cont_2 description:Wildtype and SIPK -silenced (RNAi) tobacco cell suspension cultures were grown in MS medium at 25 C in the dark. Cell suspension cultures were transferred to fresh growth medium 3 days prior to pooling each genotype and dividing the pool into 3 x 10ml volumes per genotype. A 30 minute pre-incubation was used with xanthine (for abiotic controls and treatments), but no pre-treatment was used for the biotic stressors. For each treatment and abiotic/biotic controls, the cells were harvested by vacuum filtration at the appropriate time points (controls sampled at 0 hrs) and frozen in N2(l) for RNA extraction. In order to discern the effect of silencing SIPK on the transciptome in defence response, biological replicates of WT and SIPK-Ri were randomly paired on 3 slides per treatment or control group. Keywords = Nicotiana tabacum, Cell suspension culture, Biotic stress
18 GSM51649 WT_vs_SIPK-Ri_ab_cont_3 32,448 The Institute for Genomic Research 2005-05-17 [cDNA Array] Potato 10k cDNA array version 2 (GPL1901) RNA Nicotiana tabacum
Nicotiana tabacum
unclassified source_name:Query sample; Genotype: Xanthi SIPK-Ri; Tissue: Cell suspension culture; Treatment: Abiotic biological control, 0 h; CH2: Cy3 Reference sample; Genotype: Xanthi; Tissue: Cell suspension culture; Treatment: Abiotic biological control, 0 h; CH1: Cy5 title:WT_vs_SIPK-Ri_ab_cont_3 description:Wildtype and SIPK -silenced (RNAi) tobacco cell suspension cultures were grown in MS medium at 25 C in the dark. Cell suspension cultures were transferred to fresh growth medium 3 days prior to pooling each genotype and dividing the pool into 3 x 10ml volumes per genotype. A 30 minute pre-incubation was used with xanthine (for abiotic controls and treatments), but no pre-treatment was used for the biotic stressors. For each treatment and abiotic/biotic controls, the cells were harvested by vacuum filtration at the appropriate time points (controls sampled at 0 hrs) and frozen in N2(l) for RNA extraction. In order to discern the effect of silencing SIPK on the transciptome in defence response, biological replicates of WT and SIPK-Ri were randomly paired on 3 slides per treatment or control group. Keywords = Nicotiana tabacum, Cell suspension culture, Biotic stress
19 GSM51650 WT_vs_SIPK-Ri_4h_xox_1 32,448 The Institute for Genomic Research 2005-05-17 [cDNA Array] Potato 10k cDNA array version 2 (GPL1901) RNA Nicotiana tabacum
Nicotiana tabacum
unclassified source_name:Query sample; Genotype: Xanthi SIPK-Ri; Tissue: Cell suspension culture; Treatment: Xanthine:xanthine oxidase, 4 h; CH2: Cy3 Reference sample; Genotype: Xanthi; Tissue: Cell suspension culture; Treatment: Xanthine:xanthine oxidase, 4 h; CH1: Cy5 title:WT_vs_SIPK-Ri_4h_xox_1 description:Wildtype and SIPK -silenced (RNAi) tobacco cell suspension cultures were grown in MS medium at 25 C in the dark. Cell suspension cultures were transferred to fresh growth medium 3 days prior to pooling each genotype and dividing the pool into 3 x 10ml volumes per genotype. A 30 minute pre-incubation was used with xanthine (for abiotic controls and treatments), but no pre-treatment was used for the biotic stressors. For each treatment and abiotic/biotic controls, the cells were harvested by vacuum filtration at the appropriate time points (controls sampled at 0 hrs) and frozen in N2(l) for RNA extraction. In order to discern the effect of silencing SIPK on the transciptome in defence response, biological replicates of WT and SIPK-Ri were randomly paired on 3 slides per treatment or control group. Keywords = Nicotiana tabacum, Cell suspension culture, Biotic stress
20 GSM51651 WT_vs_SIPK-Ri_4h_xox_2 32,448 The Institute for Genomic Research 2005-05-17 [cDNA Array] Potato 10k cDNA array version 2 (GPL1901) RNA Nicotiana tabacum
Nicotiana tabacum
unclassified source_name:Query sample; Genotype: Xanthi SIPK-Ri; Tissue: Cell suspension culture; Treatment: Xanthine:xanthine oxidase, 4 h; CH2: Cy3 Reference sample; Genotype: Xanthi; Tissue: Cell suspension culture; Treatment: Xanthine:xanthine oxidase, 4 h; CH1: Cy5 title:WT_vs_SIPK-Ri_4h_xox_2 description:Wildtype and SIPK -silenced (RNAi) tobacco cell suspension cultures were grown in MS medium at 25 C in the dark. Cell suspension cultures were transferred to fresh growth medium 3 days prior to pooling each genotype and dividing the pool into 3 x 10ml volumes per genotype. A 30 minute pre-incubation was used with xanthine (for abiotic controls and treatments), but no pre-treatment was used for the biotic stressors. For each treatment and abiotic/biotic controls, the cells were harvested by vacuum filtration at the appropriate time points (controls sampled at 0 hrs) and frozen in N2(l) for RNA extraction. In order to discern the effect of silencing SIPK on the transciptome in defence response, biological replicates of WT and SIPK-Ri were randomly paired on 3 slides per treatment or control group. Keywords = Nicotiana tabacum, Cell suspension culture, Biotic stress
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