Gene Expression Omnibus (GEO) Overview Version：2013-04-06Japanese page
An overview of the GEO entries broken down by the measurement platforms and the features of the measured samples.
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Data Unit : [ DataSet / Sample / Platform ] Show explanation>> <<Hide explanation
DataSet : Series(GSE) x Platform(GPL). A set of related gene expression data.
Sample : Biological materials.
platform : Methods or instruments used for the gene expression profilings.
The numbers shown in the tabs are the numbers of the data (series, samples or platforms) belonging to the groups.
  Human
(2,851)		   Primates
(60)		   Rodents
(2,590)		   Mammals
(41)		   Vertebrates
(39)		   Invertebrates
(59)		   Plants
(464)		   Bacteria
(78)		   Viruses
(0)		   Phages
(0)		   Unclassified
(120)		   All
(6,302)		  
  SAGE NlaIII
(5)		   SAGE RsaI
(0)		   SAGE Sau3A
(0)		   MPSS
(0)		   GeneChip
(132)		   Tiling Array
(15)		   cDNA Array
(222)		   Oligo Array
(90)		   Bead Array
(0)		   Protein Array
(0)		   Antibody
(0)		   RT-PCR
(0)		   HT-Seq
(0)		   Other
(0)		   All
(464)		  
  brain
(0)		   blood
(0)		   connective
(0)		   reproductive
(0)		   muscular
(0)		   digestive
(0)		   liver
(0)		   lung
(0)		   urinary
(0)		   endo/exo-crine
(0)		   embryo
(0)		   adult aerial structure
(23)		   young aerial structure
(0)		   root
(0)		   meristem/growing tissue
(12)		   flower/sexual organ
(6)		   seed/fruit/grain
(0)		   pooled
(76)		   unclassified
(347)		   all
(464)		  
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Sample ID Title Number of Data Institute Submission date Platform Sample type Species Organ class Reasoning of the classification
Keywords used for the classification are shown with bold font.
1 GSM34948 150600 14,112 The University of Iowa 2004-11-10 [Oligo Array] Soll albicans (GPL1519) RNA Candida albicans
Candida albicans		 unclassified source_name:mixed a/a-alpha/alpha cell culture, 7.5 hr homogeneous alpha/alpha cells, 3.5 hr title:150600 description:opaque a/a cells of strain P37005 and opaque alpha/alpha cells of strain WO-1, individually grown to stationary phase in modified Lee's medium at 25ºC for 48 hr cells pelleted and resuspended in fresh modified Lee's medium at original stationary phase cell concentration under following conditions 1) homogeneous a/a cells, 3.5 hr 2) homogeneous alpha/alpha cells, 3.5 hr 3) mixed a/a-alpha/alpha cell cultures, 3.5 hr and 7.5 hr The cDNA from target-source1 and target-source2 was indirectly coupled separately with Cy5 (1) and Cy3 (2) monoreactive fluors, respectively, in 0.05 M sodium bicarbonate, pH 9.0. The Cy5 and Cy3 labelled cDNAs were purified with a QIAquick PCR purification kit, quantified, combined, and suspended in 30 µl of hybridization buffer (10X SSC and 0.2% SDS) and applied to microarray slides Fluorescent array images were collected for both Cy5 and Cy3 channels with a GenePix 4000B fluorescent scanner, image intensity data was extracted with Genepix Pro 4.0
2 GSM35063 150601 14,112 The University of Iowa 2004-11-11 [Oligo Array] Soll albicans (GPL1519) RNA Candida albicans
Candida albicans		 unclassified source_name:homogeneous alpha/alpha cells, 3.5 hr mixed a/a-alpha/alpha cell culture, 7.5 hr title:150601 description:opaque a/a cells of strain P37005 and opaque alpha/alpha cells of strain WO-1, individually grown to stationary phase in modified Lee's medium at 25ºC for 48 hr cells pelleted and resuspended in fresh modified Lee's medium at original stationary phase cell concentration under following conditions 1) homogeneous a/a cells, 3.5 hr 2) homogeneous alpha/alpha cells, 3.5 hr 3) mixed a/a-alpha/alpha cell cultures, 3.5 hr and 7.5 hr The cDNA from target-source1 and target-source2 was indirectly coupled separately with Cy5 (1) and Cy3 (2) monoreactive fluors, respectively, in 0.05 M sodium bicarbonate, pH 9.0. The Cy5 and Cy3 labelled cDNAs were purified with a QIAquick PCR purification kit, quantified, combined, and suspended in 30 µl of hybridization buffer (10X SSC and 0.2% SDS) and applied to microarray slides Fluorescent array images were collected for both Cy5 and Cy3 channels with a GenePix 4000B fluorescent scanner, image intensity data was extracted with Genepix Pro 4.0
3 GSM35064 150602 14,112 The University of Iowa 2004-11-11 [Oligo Array] Soll albicans (GPL1519) RNA Candida albicans
Candida albicans		 unclassified source_name:mixed a/a-alpha/alpha cell culture, 7.5 hr homogeneous a/a cells, 3.5 hr title:150602 description:opaque a/a cells of strain P37005 and opaque alpha/alpha cells of strain WO-1, individually grown to stationary phase in modified Lee's medium at 25ºC for 48 hr cells pelleted and resuspended in fresh modified Lee's medium at original stationary phase cell concentration under following conditions 1) homogeneous a/a cells, 3.5 hr 2) homogeneous alpha/alpha cells, 3.5 hr 3) mixed a/a-alpha/alpha cell cultures, 3.5 hr and 7.5 hr The cDNA from target-source1 and target-source2 was indirectly coupled separately with Cy5 (1) and Cy3 (2) monoreactive fluors, respectively, in 0.05 M sodium bicarbonate, pH 9.0. The Cy5 and Cy3 labelled cDNAs were purified with a QIAquick PCR purification kit, quantified, combined, and suspended in 30 µl of hybridization buffer (10X SSC and 0.2% SDS) and applied to microarray slides Fluorescent array images were collected for both Cy5 and Cy3 channels with a GenePix 4000B fluorescent scanner, image intensity data was extracted with Genepix Pro 4.0
4 GSM35065 150603 14,112 The University of Iowa 2004-11-11 [Oligo Array] Soll albicans (GPL1519) RNA Candida albicans
Candida albicans		 unclassified source_name:homogeneous a/a cells, 3.5 hr mixed a/a-alpha/alpha cell culture, 7.5 hr title:150603 description:opaque a/a cells of strain P37005 and opaque alpha/alpha cells of strain WO-1, individually grown to stationary phase in modified Lee's medium at 25ºC for 48 hr cells pelleted and resuspended in fresh modified Lee's medium at original stationary phase cell concentration under following conditions 1) homogeneous a/a cells, 3.5 hr 2) homogeneous alpha/alpha cells, 3.5 hr 3) mixed a/a-alpha/alpha cell cultures, 3.5 hr and 7.5 hr The cDNA from target-source1 and target-source2 was indirectly coupled separately with Cy5 (1) and Cy3 (2) monoreactive fluors, respectively, in 0.05 M sodium bicarbonate, pH 9.0. The Cy5 and Cy3 labelled cDNAs were purified with a QIAquick PCR purification kit, quantified, combined, and suspended in 30 µl of hybridization buffer (10X SSC and 0.2% SDS) and applied to microarray slides Fluorescent array images were collected for both Cy5 and Cy3 channels with a GenePix 4000B fluorescent scanner, image intensity data was extracted with Genepix Pro 4.0
5 GSM35066 150604 14,112 The University of Iowa 2004-11-11 [Oligo Array] Soll albicans (GPL1519) RNA Candida albicans
Candida albicans		 unclassified source_name:mixed a/a-alpha/alpha cell culture, 7.5 hr homogeneous alpha/alpha cells, 3.5 hr title:150604 description:opaque a/a cells of strain P37005 and opaque alpha/alpha cells of strain WO-1, individually grown to stationary phase in modified Lee's medium at 25ºC for 48 hr cells pelleted and resuspended in fresh modified Lee's medium at original stationary phase cell concentration under following conditions 1) homogeneous a/a cells, 3.5 hr 2) homogeneous alpha/alpha cells, 3.5 hr 3) mixed a/a-alpha/alpha cell cultures, 3.5 hr and 7.5 hr The cDNA from target-source1 and target-source2 was indirectly coupled separately with Cy5 (1) and Cy3 (2) monoreactive fluors, respectively, in 0.05 M sodium bicarbonate, pH 9.0. The Cy5 and Cy3 labelled cDNAs were purified with a QIAquick PCR purification kit, quantified, combined, and suspended in 30 µl of hybridization buffer (10X SSC and 0.2% SDS) and applied to microarray slides Fluorescent array images were collected for both Cy5 and Cy3 channels with a GenePix 4000B fluorescent scanner, image intensity data was extracted with Genepix Pro 4.0
6 GSM35067 150605 14,112 The University of Iowa 2004-11-11 [Oligo Array] Soll albicans (GPL1519) RNA Candida albicans
Candida albicans		 unclassified source_name:homogeneous alpha/alpha cells, 3.5 hr mixed a/a-alpha/alpha cell culture, 7.5 hr title:150605 description:opaque a/a cells of strain P37005 and opaque alpha/alpha cells of strain WO-1, individually grown to stationary phase in modified Lee's medium at 25ºC for 48 hr cells pelleted and resuspended in fresh modified Lee's medium at original stationary phase cell concentration under following conditions 1) homogeneous a/a cells, 3.5 hr 2) homogeneous alpha/alpha cells, 3.5 hr 3) mixed a/a-alpha/alpha cell cultures, 3.5 hr and 7.5 hr The cDNA from target-source1 and target-source2 was indirectly coupled separately with Cy5 (1) and Cy3 (2) monoreactive fluors, respectively, in 0.05 M sodium bicarbonate, pH 9.0. The Cy5 and Cy3 labelled cDNAs were purified with a QIAquick PCR purification kit, quantified, combined, and suspended in 30 µl of hybridization buffer (10X SSC and 0.2% SDS) and applied to microarray slides Fluorescent array images were collected for both Cy5 and Cy3 channels with a GenePix 4000B fluorescent scanner, image intensity data was extracted with Genepix Pro 4.0
7 GSM35068 150606 14,112 The University of Iowa 2004-11-11 [Oligo Array] Soll albicans (GPL1519) RNA Candida albicans
Candida albicans		 unclassified source_name:homogeneous a/a cells, 3.5 hr homogeneous alpha/alpha cells, 3.5 hr title:150606 description:opaque a/a cells of strain P37005 and opaque alpha/alpha cells of strain WO-1, individually grown to stationary phase in modified Lee's medium at 25ºC for 48 hr cells pelleted and resuspended in fresh modified Lee's medium at original stationary phase cell concentration under following conditions 1) homogeneous a/a cells, 3.5 hr 2) homogeneous alpha/alpha cells, 3.5 hr 3) mixed a/a-alpha/alpha cell cultures, 3.5 hr and 7.5 hr The cDNA from target-source1 and target-source2 was indirectly coupled separately with Cy5 (1) and Cy3 (2) monoreactive fluors, respectively, in 0.05 M sodium bicarbonate, pH 9.0. The Cy5 and Cy3 labelled cDNAs were purified with a QIAquick PCR purification kit, quantified, combined, and suspended in 30 µl of hybridization buffer (10X SSC and 0.2% SDS) and applied to microarray slides Fluorescent array images were collected for both Cy5 and Cy3 channels with a GenePix 4000B fluorescent scanner, image intensity data was extracted with Genepix Pro 4.0
8 GSM35069 150607 14,112 The University of Iowa 2004-11-11 [Oligo Array] Soll albicans (GPL1519) RNA Candida albicans
Candida albicans		 unclassified source_name:homogeneous alpha/alpha cells, 3.5 hr homogeneous a/a cells, 3.5 hr title:150607 description:opaque a/a cells of strain P37005 and opaque alpha/alpha cells of strain WO-1, individually grown to stationary phase in modified Lee's medium at 25ºC for 48 hr cells pelleted and resuspended in fresh modified Lee's medium at original stationary phase cell concentration under following conditions 1) homogeneous a/a cells, 3.5 hr 2) homogeneous alpha/alpha cells, 3.5 hr 3) mixed a/a-alpha/alpha cell cultures, 3.5 hr and 7.5 hr The cDNA from target-source1 and target-source2 was indirectly coupled separately with Cy5 (1) and Cy3 (2) monoreactive fluors, respectively, in 0.05 M sodium bicarbonate, pH 9.0. The Cy5 and Cy3 labelled cDNAs were purified with a QIAquick PCR purification kit, quantified, combined, and suspended in 30 µl of hybridization buffer (10X SSC and 0.2% SDS) and applied to microarray slides Fluorescent array images were collected for both Cy5 and Cy3 channels with a GenePix 4000B fluorescent scanner, image intensity data was extracted with Genepix Pro 4.0
9 GSM35070 150608 14,112 The University of Iowa 2004-11-11 [Oligo Array] Soll albicans (GPL1519) RNA Candida albicans
Candida albicans		 unclassified source_name:homogeneous a/a cells, 3.5 hr homogeneous alpha/alpha cells, 3.5 hr title:150608 description:opaque a/a cells of strain P37005 and opaque alpha/alpha cells of strain WO-1, individually grown to stationary phase in modified Lee's medium at 25ºC for 48 hr cells pelleted and resuspended in fresh modified Lee's medium at original stationary phase cell concentration under following conditions 1) homogeneous a/a cells, 3.5 hr 2) homogeneous alpha/alpha cells, 3.5 hr 3) mixed a/a-alpha/alpha cell cultures, 3.5 hr and 7.5 hr The cDNA from target-source1 and target-source2 was indirectly coupled separately with Cy5 (1) and Cy3 (2) monoreactive fluors, respectively, in 0.05 M sodium bicarbonate, pH 9.0. The Cy5 and Cy3 labelled cDNAs were purified with a QIAquick PCR purification kit, quantified, combined, and suspended in 30 µl of hybridization buffer (10X SSC and 0.2% SDS) and applied to microarray slides Fluorescent array images were collected for both Cy5 and Cy3 channels with a GenePix 4000B fluorescent scanner, image intensity data was extracted with Genepix Pro 4.0
10 GSM35071 150609 14,112 The University of Iowa 2004-11-11 [Oligo Array] Soll albicans (GPL1519) RNA Candida albicans
Candida albicans		 unclassified source_name:homogeneous alpha/alpha cells, 3.5 hr homogeneous a/a cells, 3.5 hr title:150609 description:opaque a/a cells of strain P37005 and opaque alpha/alpha cells of strain WO-1, individually grown to stationary phase in modified Lee's medium at 25ºC for 48 hr cells pelleted and resuspended in fresh modified Lee's medium at original stationary phase cell concentration under following conditions 1) homogeneous a/a cells, 3.5 hr 2) homogeneous alpha/alpha cells, 3.5 hr 3) mixed a/a-alpha/alpha cell cultures, 3.5 hr and 7.5 hr The cDNA from target-source1 and target-source2 was indirectly coupled separately with Cy5 (1) and Cy3 (2) monoreactive fluors, respectively, in 0.05 M sodium bicarbonate, pH 9.0. The Cy5 and Cy3 labelled cDNAs were purified with a QIAquick PCR purification kit, quantified, combined, and suspended in 30 µl of hybridization buffer (10X SSC and 0.2% SDS) and applied to microarray slides Fluorescent array images were collected for both Cy5 and Cy3 channels with a GenePix 4000B fluorescent scanner, image intensity data was extracted with Genepix Pro 4.0
11 GSM35072 150610 14,112 The University of Iowa 2004-11-11 [Oligo Array] Soll albicans (GPL1519) RNA Candida albicans
Candida albicans		 unclassified source_name:mixed a/a-alpha/alpha cell cultures, 7.5 hr homogeneous a/a cells, 3.5 hr title:150610 description:opaque a/a cells of strain P37005 and opaque alpha/alpha cells of strain WO-1, individually grown to stationary phase in modified Lee's medium at 25ºC for 48 hr cells pelleted and resuspended in fresh modified Lee's medium at original stationary phase cell concentration under following conditions 1) homogeneous a/a cells, 3.5 hr 2) homogeneous alpha/alpha cells, 3.5 hr 3) mixed a/a-alpha/alpha cell cultures, 3.5 hr and 7.5 hr The cDNA from target-source1 and target-source2 was indirectly coupled separately with Cy5 (1) and Cy3 (2) monoreactive fluors, respectively, in 0.05 M sodium bicarbonate, pH 9.0. The Cy5 and Cy3 labelled cDNAs were purified with a QIAquick PCR purification kit, quantified, combined, and suspended in 30 µl of hybridization buffer (10X SSC and 0.2% SDS) and applied to microarray slides Fluorescent array images were collected for both Cy5 and Cy3 channels with a GenePix 4000B fluorescent scanner, image intensity data was extracted with Genepix Pro 4.0
12 GSM35073 150611 14,112 The University of Iowa 2004-11-11 [Oligo Array] Soll albicans (GPL1519) RNA Candida albicans
Candida albicans		 unclassified source_name:homogeneous a/a cells, 3.5 hr mixed a/a-alpha/alpha cell cultures, 7.5 hr title:150611 description:opaque a/a cells of strain P37005 and opaque alpha/alpha cells of strain WO-1, individually grown to stationary phase in modified Lee's medium at 25ºC for 48 hr cells pelleted and resuspended in fresh modified Lee's medium at original stationary phase cell concentration under following conditions 1) homogeneous a/a cells, 3.5 hr 2) homogeneous alpha/alpha cells, 3.5 hr 3) mixed a/a-alpha/alpha cell cultures, 3.5 hr and 7.5 hr The cDNA from target-source1 and target-source2 was indirectly coupled separately with Cy5 (1) and Cy3 (2) monoreactive fluors, respectively, in 0.05 M sodium bicarbonate, pH 9.0. The Cy5 and Cy3 labelled cDNAs were purified with a QIAquick PCR purification kit, quantified, combined, and suspended in 30 µl of hybridization buffer (10X SSC and 0.2% SDS) and applied to microarray slides Fluorescent array images were collected for both Cy5 and Cy3 channels with a GenePix 4000B fluorescent scanner, image intensity data was extracted with Genepix Pro 4.0
13 GSM35089 150618 14,112 The University of Iowa 2004-11-11 [Oligo Array] Soll albicans (GPL1519) RNA Candida albicans
Candida albicans		 unclassified source_name:mixed a/a-alpha/alpha cell cultures, 3.5 hr homogeneous a/a cells, 3.5 hr title:150618 description:opaque a/a cells of strain P37005 and opaque alpha/alpha cells of strain WO-1, individually grown to stationary phase in modified Lee's medium at 25ºC for 48 hr cells pelleted and resuspended in fresh modified Lee's medium at original stationary phase cell concentration under following conditions 1) homogeneous a/a cells, 3.5 hr 2) homogeneous alpha/alpha cells, 3.5 hr 3) mixed a/a-alpha/alpha cell cultures, 3.5 hr and 7.5 hr The cDNA from target-source1 and target-source2 was indirectly coupled separately with Cy5 (1) and Cy3 (2) monoreactive fluors, respectively, in 0.05 M sodium bicarbonate, pH 9.0. The Cy5 and Cy3 labelled cDNAs were purified with a QIAquick PCR purification kit, quantified, combined, and suspended in 30 µl of hybridization buffer (10X SSC and 0.2% SDS) and applied to microarray slides Fluorescent array images were collected for both Cy5 and Cy3 channels with a GenePix 4000B fluorescent scanner, image intensity data was extracted with Genepix Pro 4.0
14 GSM35090 150619 14,112 The University of Iowa 2004-11-11 [Oligo Array] Soll albicans (GPL1519) RNA Candida albicans
Candida albicans		 unclassified source_name:homogeneous a/a cells, 3.5 hr mixed a/a-alpha/alpha cell cultures, 3.5 hr title:150619 description:opaque a/a cells of strain P37005 and opaque alpha/alpha cells of strain WO-1, individually grown to stationary phase in modified Lee's medium at 25ºC for 48 hr cells pelleted and resuspended in fresh modified Lee's medium at original stationary phase cell concentration under following conditions 1) homogeneous a/a cells, 3.5 hr 2) homogeneous alpha/alpha cells, 3.5 hr 3) mixed a/a-alpha/alpha cell cultures, 3.5 hr and 7.5 hr The cDNA from target-source1 and target-source2 was indirectly coupled separately with Cy5 (1) and Cy3 (2) monoreactive fluors, respectively, in 0.05 M sodium bicarbonate, pH 9.0. The Cy5 and Cy3 labelled cDNAs were purified with a QIAquick PCR purification kit, quantified, combined, and suspended in 30 µl of hybridization buffer (10X SSC and 0.2% SDS) and applied to microarray slides Fluorescent array images were collected for both Cy5 and Cy3 channels with a GenePix 4000B fluorescent scanner, image intensity data was extracted with Genepix Pro 4.0
15 GSM35091 150626 14,112 The University of Iowa 2004-11-11 [Oligo Array] Soll albicans (GPL1519) RNA Candida albicans
Candida albicans		 unclassified source_name:mixed a/a-alpha/alpha cell cultures, 7.5 hr homogeneous alpha/alpha cells, 3.5 hr title:150626 description:opaque a/a cells of strain P37005 and opaque alpha/alpha cells of strain WO-1, individually grown to stationary phase in modified Lee's medium at 25ºC for 48 hr cells pelleted and resuspended in fresh modified Lee's medium at original stationary phase cell concentration under following conditions 1) homogeneous a/a cells, 3.5 hr 2) homogeneous alpha/alpha cells, 3.5 hr 3) mixed a/a-alpha/alpha cell cultures, 3.5 hr and 7.5 hr The cDNA from target-source1 and target-source2 was indirectly coupled separately with Cy5 (1) and Cy3 (2) monoreactive fluors, respectively, in 0.05 M sodium bicarbonate, pH 9.0. The Cy5 and Cy3 labelled cDNAs were purified with a QIAquick PCR purification kit, quantified, combined, and suspended in 30 µl of hybridization buffer (10X SSC and 0.2% SDS) and applied to microarray slides Fluorescent array images were collected for both Cy5 and Cy3 channels with a GenePix 4000B fluorescent scanner, image intensity data was extracted with Genepix Pro 4.0
16 GSM35092 150627 14,112 The University of Iowa 2004-11-11 [Oligo Array] Soll albicans (GPL1519) RNA Candida albicans
Candida albicans		 unclassified source_name:homogeneous alpha/alpha cells, 3.5 hr mixed a/a-alpha/alpha cell cultures, 7.5 hr title:150627 description:opaque a/a cells of strain P37005 and opaque alpha/alpha cells of strain WO-1, individually grown to stationary phase in modified Lee's medium at 25ºC for 48 hr cells pelleted and resuspended in fresh modified Lee's medium at original stationary phase cell concentration under following conditions 1) homogeneous a/a cells, 3.5 hr 2) homogeneous alpha/alpha cells, 3.5 hr 3) mixed a/a-alpha/alpha cell cultures, 3.5 hr and 7.5 hr The cDNA from target-source1 and target-source2 was indirectly coupled separately with Cy5 (1) and Cy3 (2) monoreactive fluors, respectively, in 0.05 M sodium bicarbonate, pH 9.0. The Cy5 and Cy3 labelled cDNAs were purified with a QIAquick PCR purification kit, quantified, combined, and suspended in 30 µl of hybridization buffer (10X SSC and 0.2% SDS) and applied to microarray slides Fluorescent array images were collected for both Cy5 and Cy3 channels with a GenePix 4000B fluorescent scanner, image intensity data was extracted with Genepix Pro 4.0
17 GSM35129 150630 14,112 The University of Iowa 2004-11-11 [Oligo Array] Soll albicans (GPL1519) RNA Candida albicans
Candida albicans		 unclassified source_name:mixed a/a-alpha/alpha cell cultures, 3.5 hr homogeneous alpha/alpha cells, 3.5 hr title:150630 description:opaque a/a cells of strain P37005 and opaque alpha/alpha cells of strain WO-1, individually grown to stationary phase in modified Lee's medium at 25ºC for 48 hr cells pelleted and resuspended in fresh modified Lee's medium at original stationary phase cell concentration under following conditions 1) homogeneous a/a cells, 3.5 hr 2) homogeneous alpha/alpha cells, 3.5 hr 3) mixed a/a-alpha/alpha cell cultures, 3.5 hr and 7.5 hr The cDNA from target-source1 and target-source2 was indirectly coupled separately with Cy5 (1) and Cy3 (2) monoreactive fluors, respectively, in 0.05 M sodium bicarbonate, pH 9.0. The Cy5 and Cy3 labelled cDNAs were purified with a QIAquick PCR purification kit, quantified, combined, and suspended in 30 µl of hybridization buffer (10X SSC and 0.2% SDS) and applied to microarray slides Fluorescent array images were collected for both Cy5 and Cy3 channels with a GenePix 4000B fluorescent scanner, image intensity data was extracted with Genepix Pro 4.0
18 GSM35131 150631 14,112 The University of Iowa 2004-11-11 [Oligo Array] Soll albicans (GPL1519) RNA Candida albicans
Candida albicans		 unclassified source_name:homogeneous alpha/alpha cells, 3.5 hr mixed a/a-alpha/alpha cell cultures, 3.5 hr title:150631 description:opaque a/a cells of strain P37005 and opaque alpha/alpha cells of strain WO-1, individually grown to stationary phase in modified Lee's medium at 25ºC for 48 hr cells pelleted and resuspended in fresh modified Lee's medium at original stationary phase cell concentration under following conditions 1) homogeneous a/a cells, 3.5 hr 2) homogeneous alpha/alpha cells, 3.5 hr 3) mixed a/a-alpha/alpha cell cultures, 3.5 hr and 7.5 hr The cDNA from target-source1 and target-source2 was indirectly coupled separately with Cy5 (1) and Cy3 (2) monoreactive fluors, respectively, in 0.05 M sodium bicarbonate, pH 9.0. The Cy5 and Cy3 labelled cDNAs were purified with a QIAquick PCR purification kit, quantified, combined, and suspended in 30 µl of hybridization buffer (10X SSC and 0.2% SDS) and applied to microarray slides Fluorescent array images were collected for both Cy5 and Cy3 channels with a GenePix 4000B fluorescent scanner, image intensity data was extracted with Genepix Pro 4.0
19 GSM35132 150636 14,112 The University of Iowa 2004-11-11 [Oligo Array] Soll albicans (GPL1519) RNA Candida albicans
Candida albicans		 unclassified source_name:mixed a/a-alpha/alpha cell cultures, 7.5 hr homogeneous a/a cells, 3.5 hr title:150636 description:opaque a/a cells of strain P37005 and opaque alpha/alpha cells of strain WO-1, individually grown to stationary phase in modified Lee's medium at 25ºC for 48 hr cells pelleted and resuspended in fresh modified Lee's medium at original stationary phase cell concentration under following conditions 1) homogeneous a/a cells, 3.5 hr 2) homogeneous alpha/alpha cells, 3.5 hr 3) mixed a/a-alpha/alpha cell cultures, 3.5 hr and 7.5 hr The cDNA from target-source1 and target-source2 was indirectly coupled separately with Cy5 (1) and Cy3 (2) monoreactive fluors, respectively, in 0.05 M sodium bicarbonate, pH 9.0. The Cy5 and Cy3 labelled cDNAs were purified with a QIAquick PCR purification kit, quantified, combined, and suspended in 30 µl of hybridization buffer (10X SSC and 0.2% SDS) and applied to microarray slides Fluorescent array images were collected for both Cy5 and Cy3 channels with a GenePix 4000B fluorescent scanner, image intensity data was extracted with Genepix Pro 4.0
20 GSM35134 150637 14,112 The University of Iowa 2004-11-11 [Oligo Array] Soll albicans (GPL1519) RNA Candida albicans
Candida albicans		 unclassified source_name:homogeneous a/a cells, 3.5 hr mixed a/a-alpha/alpha cell cultures, 7.5 hr title:150637 description:opaque a/a cells of strain P37005 and opaque alpha/alpha cells of strain WO-1, individually grown to stationary phase in modified Lee's medium at 25ºC for 48 hr cells pelleted and resuspended in fresh modified Lee's medium at original stationary phase cell concentration under following conditions 1) homogeneous a/a cells, 3.5 hr 2) homogeneous alpha/alpha cells, 3.5 hr 3) mixed a/a-alpha/alpha cell cultures, 3.5 hr and 7.5 hr The cDNA from target-source1 and target-source2 was indirectly coupled separately with Cy5 (1) and Cy3 (2) monoreactive fluors, respectively, in 0.05 M sodium bicarbonate, pH 9.0. The Cy5 and Cy3 labelled cDNAs were purified with a QIAquick PCR purification kit, quantified, combined, and suspended in 30 µl of hybridization buffer (10X SSC and 0.2% SDS) and applied to microarray slides Fluorescent array images were collected for both Cy5 and Cy3 channels with a GenePix 4000B fluorescent scanner, image intensity data was extracted with Genepix Pro 4.0
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