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[cDNA Array] Potato 10k cDNA array version 2 (GPL1901)
RNA
Nicotiana tabacum
unclassified
source_name:Query sample; Genotype: Xanthi SIPK-Ri; Tissue: Cell suspension culture; Treatment: Biotic control, 0 h; CH2: Cy3 Reference sample; Genotype: Xanthi; Tissue: Cell suspension culture; Treatment: Biotic control, 0 h; CH1: Cy5 title:WT_vs_SIPK-Ri_b_cont_1 description:Wildtype and SIPK -silenced (RNAi) tobacco cell suspension cultures were grown in MS medium at 25 C in the dark. Cell suspension cultures were transferred to fresh growth medium 3 days prior to pooling each genotype and dividing the pool into 3 x 10ml volumes per genotype. A 30 minute pre-incubation was used with xanthine (for abiotic controls and treatments), but no pre-treatment was used for the biotic stressors. For each treatment and abiotic/biotic controls, the cells were harvested by vacuum filtration at the appropriate time points (controls sampled at 0 hrs) and frozen in N2(l) for RNA extraction. In order to discern the effect of silencing SIPK on the transciptome in defence response, biological replicates of WT and SIPK-Ri were randomly paired on 3 slides per treatment or control group. Keywords = Nicotiana tabacum, Cell suspension culture, Biotic stress
[cDNA Array] Potato 10k cDNA array version 2 (GPL1901)
RNA
Nicotiana tabacum
unclassified
source_name:Query sample; Genotype: Xanthi SIPK-Ri; Tissue: Cell suspension culture; Treatment: Biotic control, 0 h; CH2: Cy3 Reference sample; Genotype: Xanthi; Tissue: Cell suspension culture; Treatment: Biotic control, 0 h; CH1: Cy5 title:WT_vs_SIPK-Ri_b_cont_2 description:Wildtype and SIPK -silenced (RNAi) tobacco cell suspension cultures were grown in MS medium at 25 C in the dark. Cell suspension cultures were transferred to fresh growth medium 3 days prior to pooling each genotype and dividing the pool into 3 x 10ml volumes per genotype. A 30 minute pre-incubation was used with xanthine (for abiotic controls and treatments), but no pre-treatment was used for the biotic stressors. For each treatment and abiotic/biotic controls, the cells were harvested by vacuum filtration at the appropriate time points (controls sampled at 0 hrs) and frozen in N2(l) for RNA extraction. In order to discern the effect of silencing SIPK on the transciptome in defence response, biological replicates of WT and SIPK-Ri were randomly paired on 3 slides per treatment or control group. Keywords = Nicotiana tabacum, Cell suspension culture, Biotic stress
[cDNA Array] Potato 10k cDNA array version 2 (GPL1901)
RNA
Nicotiana tabacum
unclassified
source_name:Query sample; Genotype: Xanthi SIPK-Ri; Tissue: Cell suspension culture; Treatment: Biotic control, 0 h; CH2: Cy3 Reference sample; Genotype: Xanthi; Tissue: Cell suspension culture; Treatment: Biotic control, 0 h; CH1: Cy5 title:WT_vs_SIPK-Ri_b_cont_3 description:Wildtype and SIPK -silenced (RNAi) tobacco cell suspension cultures were grown in MS medium at 25 C in the dark. Cell suspension cultures were transferred to fresh growth medium 3 days prior to pooling each genotype and dividing the pool into 3 x 10ml volumes per genotype. A 30 minute pre-incubation was used with xanthine (for abiotic controls and treatments), but no pre-treatment was used for the biotic stressors. For each treatment and abiotic/biotic controls, the cells were harvested by vacuum filtration at the appropriate time points (controls sampled at 0 hrs) and frozen in N2(l) for RNA extraction. In order to discern the effect of silencing SIPK on the transciptome in defence response, biological replicates of WT and SIPK-Ri were randomly paired on 3 slides per treatment or control group. Keywords = Nicotiana tabacum, Cell suspension culture, Biotic stress
[cDNA Array] Potato 10k cDNA array version 2 (GPL1901)
RNA
Nicotiana tabacum
unclassified
source_name:Query sample; Genotype: Xanthi SIPK-Ri; Tissue: Cell suspension culture; Treatment: Harpin (P.syringae), 4 h; CH2: Cy3 Reference sample; Genotype: Xanthi; Tissue: Cell suspension culture; Treatment: Harpin (P.syringae), 4 h; CH1: Cy5 title:WT_vs_SIPK-Ri_4h_harp_1 description:Wildtype and SIPK -silenced (RNAi) tobacco cell suspension cultures were grown in MS medium at 25 C in the dark. Cell suspension cultures were transferred to fresh growth medium 3 days prior to pooling each genotype and dividing the pool into 3 x 10ml volumes per genotype. A 30 minute pre-incubation was used with xanthine (for abiotic controls and treatments), but no pre-treatment was used for the biotic stressors. For each treatment and abiotic/biotic controls, the cells were harvested by vacuum filtration at the appropriate time points (controls sampled at 0 hrs) and frozen in N2(l) for RNA extraction. In order to discern the effect of silencing SIPK on the transciptome in defence response, biological replicates of WT and SIPK-Ri were randomly paired on 3 slides per treatment or control group. Keywords = Nicotiana tabacum, Cell suspension culture, Biotic stress
[cDNA Array] Potato 10k cDNA array version 2 (GPL1901)
RNA
Nicotiana tabacum
unclassified
source_name:Query sample; Genotype: Xanthi SIPK-Ri; Tissue: Cell suspension culture; Treatment: Harpin (P.syringae), 4 h; CH2: Cy3 Reference sample; Genotype: Xanthi; Tissue: Cell suspension culture; Treatment: Harpin (P.syringae), 4 h; CH1: Cy5 title:WT_vs_SIPK-Ri_4h_harp_2 description:Wildtype and SIPK -silenced (RNAi) tobacco cell suspension cultures were grown in MS medium at 25 C in the dark. Cell suspension cultures were transferred to fresh growth medium 3 days prior to pooling each genotype and dividing the pool into 3 x 10ml volumes per genotype. A 30 minute pre-incubation was used with xanthine (for abiotic controls and treatments), but no pre-treatment was used for the biotic stressors. For each treatment and abiotic/biotic controls, the cells were harvested by vacuum filtration at the appropriate time points (controls sampled at 0 hrs) and frozen in N2(l) for RNA extraction. In order to discern the effect of silencing SIPK on the transciptome in defence response, biological replicates of WT and SIPK-Ri were randomly paired on 3 slides per treatment or control group. Keywords = Nicotiana tabacum, Cell suspension culture, Biotic stress
[cDNA Array] Potato 10k cDNA array version 2 (GPL1901)
RNA
Nicotiana tabacum
unclassified
source_name:Query sample; Genotype: Xanthi SIPK-Ri; Tissue: Cell suspension culture; Treatment: Harpin (P.syringae), 4 h; CH2: Cy3 Reference sample; Genotype: Xanthi; Tissue: Cell suspension culture; Treatment: Harpin (P.syringae), 4 h; CH1: Cy5 title:WT_vs_SIPK-Ri_4h_harp_3 description:Wildtype and SIPK -silenced (RNAi) tobacco cell suspension cultures were grown in MS medium at 25 C in the dark. Cell suspension cultures were transferred to fresh growth medium 3 days prior to pooling each genotype and dividing the pool into 3 x 10ml volumes per genotype. A 30 minute pre-incubation was used with xanthine (for abiotic controls and treatments), but no pre-treatment was used for the biotic stressors. For each treatment and abiotic/biotic controls, the cells were harvested by vacuum filtration at the appropriate time points (controls sampled at 0 hrs) and frozen in N2(l) for RNA extraction. In order to discern the effect of silencing SIPK on the transciptome in defence response, biological replicates of WT and SIPK-Ri were randomly paired on 3 slides per treatment or control group. Keywords = Nicotiana tabacum, Cell suspension culture, Biotic stress
[cDNA Array] Potato 10k cDNA array version 2 (GPL1901)
RNA
Nicotiana tabacum
unclassified
source_name:Query sample; Genotype: Xanthi SIPK-Ri; Tissue: Cell suspension culture; Treatment: Megaspermin (P.megasperma), 4 h; CH2: Cy3 Reference sample; Genotype: Xanthi; Tissue: Cell suspension culture; Treatment: Megaspermin (P.megasperma), 4 h; CH1: Cy5 title:WT_vs_SIPK-Ri_4h_mega_1 description:Wildtype and SIPK -silenced (RNAi) tobacco cell suspension cultures were grown in MS medium at 25 C in the dark. Cell suspension cultures were transferred to fresh growth medium 3 days prior to pooling each genotype and dividing the pool into 3 x 10ml volumes per genotype. A 30 minute pre-incubation was used with xanthine (for abiotic controls and treatments), but no pre-treatment was used for the biotic stressors. For each treatment and abiotic/biotic controls, the cells were harvested by vacuum filtration at the appropriate time points (controls sampled at 0 hrs) and frozen in N2(l) for RNA extraction. In order to discern the effect of silencing SIPK on the transciptome in defence response, biological replicates of WT and SIPK-Ri were randomly paired on 3 slides per treatment or control group. Keywords = Nicotiana tabacum, Cell suspension culture, Biotic stress
[cDNA Array] Potato 10k cDNA array version 2 (GPL1901)
RNA
Nicotiana tabacum
unclassified
source_name:Query sample; Genotype: Xanthi SIPK-Ri; Tissue: Cell suspension culture; Treatment: Megaspermin (P.megasperma), 4 h; CH2: Cy3 Reference sample; Genotype: Xanthi; Tissue: Cell suspension culture; Treatment: Megaspermin (P.megasperma), 4 h; CH1: Cy5 title:WT_vs_SIPK-Ri_4h_mega_2 description:Wildtype and SIPK -silenced (RNAi) tobacco cell suspension cultures were grown in MS medium at 25 C in the dark. Cell suspension cultures were transferred to fresh growth medium 3 days prior to pooling each genotype and dividing the pool into 3 x 10ml volumes per genotype. A 30 minute pre-incubation was used with xanthine (for abiotic controls and treatments), but no pre-treatment was used for the biotic stressors. For each treatment and abiotic/biotic controls, the cells were harvested by vacuum filtration at the appropriate time points (controls sampled at 0 hrs) and frozen in N2(l) for RNA extraction. In order to discern the effect of silencing SIPK on the transciptome in defence response, biological replicates of WT and SIPK-Ri were randomly paired on 3 slides per treatment or control group. Keywords = Nicotiana tabacum, Cell suspension culture, Biotic stress
[cDNA Array] Potato 10k cDNA array version 2 (GPL1901)
RNA
Nicotiana tabacum
unclassified
source_name:Query sample; Genotype: Xanthi SIPK-Ri; Tissue: Cell suspension culture; Treatment: Megaspermin (P.megasperma), 4 h; CH2: Cy3 Reference sample; Genotype: Xanthi; Tissue: Cell suspension culture; Treatment: Megaspermin (P.megasperma), 4 h; CH1: Cy5 title:WT_vs_SIPK-Ri_4h_mega_3 description:Wildtype and SIPK -silenced (RNAi) tobacco cell suspension cultures were grown in MS medium at 25 C in the dark. Cell suspension cultures were transferred to fresh growth medium 3 days prior to pooling each genotype and dividing the pool into 3 x 10ml volumes per genotype. A 30 minute pre-incubation was used with xanthine (for abiotic controls and treatments), but no pre-treatment was used for the biotic stressors. For each treatment and abiotic/biotic controls, the cells were harvested by vacuum filtration at the appropriate time points (controls sampled at 0 hrs) and frozen in N2(l) for RNA extraction. In order to discern the effect of silencing SIPK on the transciptome in defence response, biological replicates of WT and SIPK-Ri were randomly paired on 3 slides per treatment or control group. Keywords = Nicotiana tabacum, Cell suspension culture, Biotic stress
[cDNA Array] Potato 10k cDNA array version 2 (GPL1901)
RNA
Nicotiana tabacum
unclassified
source_name:Query sample; Genotype: Xanthi SIPK-Ri; Tissue: Cell suspension culture; Treatment: Harpin (P.syringae), 8 h; CH2: Cy3 Reference sample; Genotype: Xanthi; Tissue: Cell suspension culture; Treatment: Harpin (P.syringae), 8 h; CH1: Cy5 title:WT_vs_SIPK-Ri_8h_harp_1 description:Wildtype and SIPK -silenced (RNAi) tobacco cell suspension cultures were grown in MS medium at 25 C in the dark. Cell suspension cultures were transferred to fresh growth medium 3 days prior to pooling each genotype and dividing the pool into 3 x 10ml volumes per genotype. A 30 minute pre-incubation was used with xanthine (for abiotic controls and treatments), but no pre-treatment was used for the biotic stressors. For each treatment and abiotic/biotic controls, the cells were harvested by vacuum filtration at the appropriate time points (controls sampled at 0 hrs) and frozen in N2(l) for RNA extraction. In order to discern the effect of silencing SIPK on the transciptome in defence response, biological replicates of WT and SIPK-Ri were randomly paired on 3 slides per treatment or control group. Keywords = Nicotiana tabacum, Cell suspension culture, Biotic stress
[cDNA Array] Potato 10k cDNA array version 2 (GPL1901)
RNA
Nicotiana tabacum
unclassified
source_name:Query sample; Genotype: Xanthi SIPK-Ri; Tissue: Cell suspension culture; Treatment: Harpin (P.syringae), 8 h; CH2: Cy3 Reference sample; Genotype: Xanthi; Tissue: Cell suspension culture; Treatment: Harpin (P.syringae), 8 h; CH1: Cy5 title:WT_vs_SIPK-Ri_8h_harp_2 description:Wildtype and SIPK -silenced (RNAi) tobacco cell suspension cultures were grown in MS medium at 25 C in the dark. Cell suspension cultures were transferred to fresh growth medium 3 days prior to pooling each genotype and dividing the pool into 3 x 10ml volumes per genotype. A 30 minute pre-incubation was used with xanthine (for abiotic controls and treatments), but no pre-treatment was used for the biotic stressors. For each treatment and abiotic/biotic controls, the cells were harvested by vacuum filtration at the appropriate time points (controls sampled at 0 hrs) and frozen in N2(l) for RNA extraction. In order to discern the effect of silencing SIPK on the transciptome in defence response, biological replicates of WT and SIPK-Ri were randomly paired on 3 slides per treatment or control group. Keywords = Nicotiana tabacum, Cell suspension culture, Biotic stress
[cDNA Array] Potato 10k cDNA array version 2 (GPL1901)
RNA
Nicotiana tabacum
unclassified
source_name:Query sample; Genotype: Xanthi SIPK-Ri; Tissue: Cell suspension culture; Treatment: Harpin (P.syringae), 8 h; CH2: Cy3 Reference sample; Genotype: Xanthi; Tissue: Cell suspension culture; Treatment: Harpin (P.syringae), 8 h; CH1: Cy5 title:WT_vs_SIPK-Ri_8h_harp_3 description:Wildtype and SIPK -silenced (RNAi) tobacco cell suspension cultures were grown in MS medium at 25 C in the dark. Cell suspension cultures were transferred to fresh growth medium 3 days prior to pooling each genotype and dividing the pool into 3 x 10ml volumes per genotype. A 30 minute pre-incubation was used with xanthine (for abiotic controls and treatments), but no pre-treatment was used for the biotic stressors. For each treatment and abiotic/biotic controls, the cells were harvested by vacuum filtration at the appropriate time points (controls sampled at 0 hrs) and frozen in N2(l) for RNA extraction. In order to discern the effect of silencing SIPK on the transciptome in defence response, biological replicates of WT and SIPK-Ri were randomly paired on 3 slides per treatment or control group. Keywords = Nicotiana tabacum, Cell suspension culture, Biotic stress
[cDNA Array] Potato 10k cDNA array version 2 (GPL1901)
RNA
Nicotiana tabacum
unclassified
source_name:Query sample; Genotype: Xanthi SIPK-Ri; Tissue: Cell suspension culture; Treatment: Megaspermin (P.megasperma), 8 h; CH2: Cy3 Reference sample; Genotype: Xanthi; Tissue: Cell suspension culture; Treatment: Megaspermin (P.megasperma), 8 h; CH1: Cy5 title:WT_vs_SIPK-Ri_8h_mega_1 description:Wildtype and SIPK -silenced (RNAi) tobacco cell suspension cultures were grown in MS medium at 25 C in the dark. Cell suspension cultures were transferred to fresh growth medium 3 days prior to pooling each genotype and dividing the pool into 3 x 10ml volumes per genotype. A 30 minute pre-incubation was used with xanthine (for abiotic controls and treatments), but no pre-treatment was used for the biotic stressors. For each treatment and abiotic/biotic controls, the cells were harvested by vacuum filtration at the appropriate time points (controls sampled at 0 hrs) and frozen in N2(l) for RNA extraction. In order to discern the effect of silencing SIPK on the transciptome in defence response, biological replicates of WT and SIPK-Ri were randomly paired on 3 slides per treatment or control group. Keywords = Nicotiana tabacum, Cell suspension culture, Biotic stress
[cDNA Array] Potato 10k cDNA array version 2 (GPL1901)
RNA
Nicotiana tabacum
unclassified
source_name:Query sample; Genotype: Xanthi SIPK-Ri; Tissue: Cell suspension culture; Treatment: Megaspermin (P.megasperma), 8 h; CH2: Cy3 Reference sample; Genotype: Xanthi; Tissue: Cell suspension culture; Treatment: Megaspermin (P.megasperma), 8 h; CH1: Cy5 title:WT_vs_SIPK-Ri_8h_mega_2 description:Wildtype and SIPK -silenced (RNAi) tobacco cell suspension cultures were grown in MS medium at 25 C in the dark. Cell suspension cultures were transferred to fresh growth medium 3 days prior to pooling each genotype and dividing the pool into 3 x 10ml volumes per genotype. A 30 minute pre-incubation was used with xanthine (for abiotic controls and treatments), but no pre-treatment was used for the biotic stressors. For each treatment and abiotic/biotic controls, the cells were harvested by vacuum filtration at the appropriate time points (controls sampled at 0 hrs) and frozen in N2(l) for RNA extraction. In order to discern the effect of silencing SIPK on the transciptome in defence response, biological replicates of WT and SIPK-Ri were randomly paired on 3 slides per treatment or control group. Keywords = Nicotiana tabacum, Cell suspension culture, Biotic stress
[cDNA Array] Potato 10k cDNA array version 2 (GPL1901)
RNA
Nicotiana tabacum
unclassified
source_name:Query sample; Genotype: Xanthi SIPK-Ri; Tissue: Cell suspension culture; Treatment: Megaspermin (P.megasperma), 8 h; CH2: Cy3 Reference sample; Genotype: Xanthi; Tissue: Cell suspension culture; Treatment: Megaspermin (P.megasperma), 8 h; CH1: Cy5 title:WT_vs_SIPK-Ri_8h_mega_3 description:Wildtype and SIPK -silenced (RNAi) tobacco cell suspension cultures were grown in MS medium at 25 C in the dark. Cell suspension cultures were transferred to fresh growth medium 3 days prior to pooling each genotype and dividing the pool into 3 x 10ml volumes per genotype. A 30 minute pre-incubation was used with xanthine (for abiotic controls and treatments), but no pre-treatment was used for the biotic stressors. For each treatment and abiotic/biotic controls, the cells were harvested by vacuum filtration at the appropriate time points (controls sampled at 0 hrs) and frozen in N2(l) for RNA extraction. In order to discern the effect of silencing SIPK on the transciptome in defence response, biological replicates of WT and SIPK-Ri were randomly paired on 3 slides per treatment or control group. Keywords = Nicotiana tabacum, Cell suspension culture, Biotic stress
[cDNA Array] Potato 10k cDNA array version 2 (GPL1901)
RNA
Nicotiana tabacum
unclassified
source_name:Query sample; Genotype: Xanthi SIPK-Ri; Tissue: Cell suspension culture; Treatment: Abiotic biological control, 0 h; CH2: Cy3 Reference sample; Genotype: Xanthi; Tissue: Cell suspension culture; Treatment: Abiotic biological control, 0 h; CH1: Cy5 title:WT_vs_SIPK-Ri_ab_cont_1 description:Wildtype and SIPK -silenced (RNAi) tobacco cell suspension cultures were grown in MS medium at 25 C in the dark. Cell suspension cultures were transferred to fresh growth medium 3 days prior to pooling each genotype and dividing the pool into 3 x 10ml volumes per genotype. A 30 minute pre-incubation was used with xanthine (for abiotic controls and treatments), but no pre-treatment was used for the biotic stressors. For each treatment and abiotic/biotic controls, the cells were harvested by vacuum filtration at the appropriate time points (controls sampled at 0 hrs) and frozen in N2(l) for RNA extraction. In order to discern the effect of silencing SIPK on the transciptome in defence response, biological replicates of WT and SIPK-Ri were randomly paired on 3 slides per treatment or control group. Keywords = Nicotiana tabacum, Cell suspension culture, Biotic stress
[cDNA Array] Potato 10k cDNA array version 2 (GPL1901)
RNA
Nicotiana tabacum
unclassified
source_name:Query sample; Genotype: Xanthi SIPK-Ri; Tissue: Cell suspension culture; Treatment: Abiotic biological control, 0 h; CH2: Cy3 Reference sample; Genotype: Xanthi; Tissue: Cell suspension culture; Treatment: Abiotic biological control, 0 h; CH1: Cy5 title:WT_vs_SIPK-Ri_ab_cont_2 description:Wildtype and SIPK -silenced (RNAi) tobacco cell suspension cultures were grown in MS medium at 25 C in the dark. Cell suspension cultures were transferred to fresh growth medium 3 days prior to pooling each genotype and dividing the pool into 3 x 10ml volumes per genotype. A 30 minute pre-incubation was used with xanthine (for abiotic controls and treatments), but no pre-treatment was used for the biotic stressors. For each treatment and abiotic/biotic controls, the cells were harvested by vacuum filtration at the appropriate time points (controls sampled at 0 hrs) and frozen in N2(l) for RNA extraction. In order to discern the effect of silencing SIPK on the transciptome in defence response, biological replicates of WT and SIPK-Ri were randomly paired on 3 slides per treatment or control group. Keywords = Nicotiana tabacum, Cell suspension culture, Biotic stress
[cDNA Array] Potato 10k cDNA array version 2 (GPL1901)
RNA
Nicotiana tabacum
unclassified
source_name:Query sample; Genotype: Xanthi SIPK-Ri; Tissue: Cell suspension culture; Treatment: Abiotic biological control, 0 h; CH2: Cy3 Reference sample; Genotype: Xanthi; Tissue: Cell suspension culture; Treatment: Abiotic biological control, 0 h; CH1: Cy5 title:WT_vs_SIPK-Ri_ab_cont_3 description:Wildtype and SIPK -silenced (RNAi) tobacco cell suspension cultures were grown in MS medium at 25 C in the dark. Cell suspension cultures were transferred to fresh growth medium 3 days prior to pooling each genotype and dividing the pool into 3 x 10ml volumes per genotype. A 30 minute pre-incubation was used with xanthine (for abiotic controls and treatments), but no pre-treatment was used for the biotic stressors. For each treatment and abiotic/biotic controls, the cells were harvested by vacuum filtration at the appropriate time points (controls sampled at 0 hrs) and frozen in N2(l) for RNA extraction. In order to discern the effect of silencing SIPK on the transciptome in defence response, biological replicates of WT and SIPK-Ri were randomly paired on 3 slides per treatment or control group. Keywords = Nicotiana tabacum, Cell suspension culture, Biotic stress
[cDNA Array] Potato 10k cDNA array version 2 (GPL1901)
RNA
Nicotiana tabacum
unclassified
source_name:Query sample; Genotype: Xanthi SIPK-Ri; Tissue: Cell suspension culture; Treatment: Xanthine:xanthine oxidase, 4 h; CH2: Cy3 Reference sample; Genotype: Xanthi; Tissue: Cell suspension culture; Treatment: Xanthine:xanthine oxidase, 4 h; CH1: Cy5 title:WT_vs_SIPK-Ri_4h_xox_1 description:Wildtype and SIPK -silenced (RNAi) tobacco cell suspension cultures were grown in MS medium at 25 C in the dark. Cell suspension cultures were transferred to fresh growth medium 3 days prior to pooling each genotype and dividing the pool into 3 x 10ml volumes per genotype. A 30 minute pre-incubation was used with xanthine (for abiotic controls and treatments), but no pre-treatment was used for the biotic stressors. For each treatment and abiotic/biotic controls, the cells were harvested by vacuum filtration at the appropriate time points (controls sampled at 0 hrs) and frozen in N2(l) for RNA extraction. In order to discern the effect of silencing SIPK on the transciptome in defence response, biological replicates of WT and SIPK-Ri were randomly paired on 3 slides per treatment or control group. Keywords = Nicotiana tabacum, Cell suspension culture, Biotic stress
[cDNA Array] Potato 10k cDNA array version 2 (GPL1901)
RNA
Nicotiana tabacum
unclassified
source_name:Query sample; Genotype: Xanthi SIPK-Ri; Tissue: Cell suspension culture; Treatment: Xanthine:xanthine oxidase, 4 h; CH2: Cy3 Reference sample; Genotype: Xanthi; Tissue: Cell suspension culture; Treatment: Xanthine:xanthine oxidase, 4 h; CH1: Cy5 title:WT_vs_SIPK-Ri_4h_xox_2 description:Wildtype and SIPK -silenced (RNAi) tobacco cell suspension cultures were grown in MS medium at 25 C in the dark. Cell suspension cultures were transferred to fresh growth medium 3 days prior to pooling each genotype and dividing the pool into 3 x 10ml volumes per genotype. A 30 minute pre-incubation was used with xanthine (for abiotic controls and treatments), but no pre-treatment was used for the biotic stressors. For each treatment and abiotic/biotic controls, the cells were harvested by vacuum filtration at the appropriate time points (controls sampled at 0 hrs) and frozen in N2(l) for RNA extraction. In order to discern the effect of silencing SIPK on the transciptome in defence response, biological replicates of WT and SIPK-Ri were randomly paired on 3 slides per treatment or control group. Keywords = Nicotiana tabacum, Cell suspension culture, Biotic stress