| 1 |
GPL1674 |
Mouse 15k cDNA array |
15,388 |
University of Washington |
2004-11-01 |
|
Mus musculus
 |
cDNA Array |
spotted DNA/cDNA, Mouse 15k cDNA array, The NIA_Mouse_15K_Array platform is composed of 15,247 cDNAs from the NIA Mouse 15K cDNA clone collection. NIA Mouse 15K is derived from several early embryonic cDNA libraries, including blastocyst, preimplantation, embryonic stem cells, trophoblast stem cells, embryonic germ cells, and others. In addition to the original NIA cloneset, the FHCRC mouse array includes 141, mostly control clones for a total of 15,388 spots on this glass microarray. Keywords = spotted, embryo stem preimplantation trophoblast blastocyst germ osteoblast |
| 2 |
GPL1480 |
MMRRC |
12,305 |
SUNY-University at Albany |
2004-10-05 |
|
Mus musculus
|
cDNA Array |
spotted DNA/cDNA, MMRRC, 9600 cDNA IMAGE consortium clones were obtained from Incyte Genomics. After amplification, they were arrayed on Corning or Fullmoon sialized slides with a GMS 417 arrayer. Cy3 and cy5 labels were scanned with a GSI Lumonics Scanarray4000XL or GenePix4000B. |
| 3 |
GPL1692 |
NIEHS Rat Chip v2.1 |
6,960 |
NIEHS |
2004-11-04 |
|
Rattus norvegicus
 |
cDNA Array |
spotted DNA/cDNA, NIEHS Rat Chip v2.1, This array was developed in-house at NIEHS, and used for gene expression profiling experiments. A complete listing of the genes on this chip is available at the following Web site: http://dir.niehs.nih.gov/microarray/chips.htm cDNA microarray chips were prepared according to DeRisi et al., 1996, Nat. Genet. 14, 457-460. The spotted cDNAs were derived from a collection of sequence-verified clones that covered the 3` end of the gene and ranged in size from 500 to 2000 base pairs (Research Genetics). M13 primers were used to amplify insert cDNAs from purified plasmid DNA in a 100 5l polymerase chain reaction (PCR) mixture. A sample of the PCR products (10 microliters) was separated on 2% agarose gels to ensure quality of the amplifications. The remaining PCR products were purified by ethanol precipitation, resuspended in ArrayIt buffer (Telechem, San Jose CA) and spotted onto poly-L-lysine-coated glass slides using a modified, robotic DNA arrayer (Beecher Instruments, Bethesda MD). -- taken largely verbatim from Hamadeh et al., Toxicological Sciences 67, 219-231 (2002) |
| 4 |
GPL1693 |
NIEHS Rat Chip v2.2 |
6,960 |
NIEHS |
2004-11-04 |
|
Rattus norvegicus
|
cDNA Array |
spotted DNA/cDNA, NIEHS Rat Chip v2.2, This array was developed in-house at NIEHS, and used for gene expression profiling experiments. A complete listing of the genes on this chip is available at the following Web site: http://dir.niehs.nih.gov/microarray/chips.htm |
| 5 |
GPL1529 |
NCI/LMT Mm_FCRF_UniGEM2 |
9,984 |
NCI |
2004-10-22 |
|
Mus musculus
|
cDNA Array |
spotted DNA/cDNA, NCI/LMT Mm_FCRF_UniGEM2, Printing by the NCI-Frederick/LMT Microarray Research Facility Surface type: non-adsorptive, polylysine-coated glass Attachment: covalent, (UV-treated polylysine) Array description: The custom arrays are manufactured by the NCI Frederick Laboratory of Molecular Technology Microarray Facility (NIH/NCI/SAIC/LMT). Microarrays are printed with BioRobotics TAS II robotic arrayer from Genomic Solutions (http://www.genomicsolutions.com) that is fitted with a printhead containing 32 SMP3 printing pins from Telechem International (http://www.arrayit.com). The mouse cDNA probe set is based on 9984 PCR products from Incyte's mouse UniGEM 2.0 library (http://www.incyte.com). The set includes 9234 Unique GenBank Accessions; 8882 mapped into Mm UniGene Build #1201; 8656 Unique UniGene Clusters; 3463 EST clusters; 5193 "Named" clusters. The cDNA probes are distributed into 384-well polypropylene plates (Genetix, 384 micro array) and dissolved in 3XSSC print buffer at a concentration of 50-100 ug/ml. The microarray spot densities are 235 microns (center-to-center), and one spot is printed per probe per slide. The microarrays are printed on Fisher Premium microscope slides (cat# 12-544-1) coated with poly-L-lysine and post-processed according to the procedures on the NCI website http://nciarray.nci.nih.gov/ . |
| 6 |
GPL1512 |
Mammary 6k and Incyte Mm GEM1 |
15,360 |
Memorial Sloan-Kettering Cancer Center |
2004-10-18 |
|
Mus musculus
|
cDNA Array |
spotted DNA/cDNA, Mammary 6k and Incyte Mm GEM1, Microarray analysis reveals changes of gene expression in the evolution of mammary tumors in Wnt-1 transgenic mice Keywords = Gene expression Keywords = hyperplasia Keywords = tumors Keywords = metastasis Keywords = Wnt-1 Keywords = Neu. |
| 7 |
GPL1511 |
Incyte GEM1 8k |
8,832 |
Memorial Sloan-Kettering Cancer Center |
2004-10-18 |
|
Mus musculus
|
cDNA Array |
spotted DNA/cDNA, Incyte GEM1 8k, Microarray analysis reveals changes of gene expression in the evolution of mammary tumors in Wnt-1 transgenic mice Keywords = Gene expression Keywords = hyperplasia Keywords = tumors Keywords = metastasis Keywords = Wnt-1 Keywords = Neu. |
| 8 |
GPL1751 |
NIH 15k clone collection/Ontario Cancer Institute |
15,249 |
Karolinska Institutet |
2004-12-16 |
|
Mus musculus
|
cDNA Array |
spotted DNA/cDNA, NIH 15k clone collection/Ontario Cancer Institute, NIH 15k mouse clone collection spotted by Ontario Cancer Institute |
| 9 |
GPL1524 |
Amersham CodeLink UniSet Mouse 20K I Bioarray |
20,289 |
Graduate School of Medicine, Kyoto University |
2004-10-21 |
|
Mus musculus
|
cDNA Array |
spotted DNA/cDNA, Amersham CodeLink UniSet Mouse 20K I Bioarray, CodeLink UniSet Mouse 20K I Bioarray targets ~20 000 unique, well-annotated mouse genes |
| 10 |
GPL1523 |
Mouse MuscleArray 1.0 |
9,216 |
CRIBI Biotechnology Center |
2004-10-21 |
|
Mus musculus
|
cDNA Array |
spotted DNA/cDNA, Mouse MuscleArray 1.0, 2,061 muscle-specific cDNA probes (300-600 bp), restricted to the 3'-end of each mRNA, were spotted in quadruplicates. MICROARRAY FABRICATION: The cDNA inserts were cloned in the plasmid vector pZErO-1 (Invitrogen) and amplified by PCR with universal primers (A: 5’-TCCGGCTCGTATGTTGTGTGG-3’, B: 5’-GTTGTAAAACGACGGCCAGTG-3’). The quality of each amplicon was monitored on agarose gels: cDNA probes showing more than a single size product, described by a ‘WARNING’ message, were excluded from subsequent microarray analyses. PCR reactions were transferred to 96-well MicroAmp Optical plates (PerkinElmer) and precipitated with ethanol and salt, following a clean-up protocol developed at the Stanford University. The microarray facility at CRIBI managed microarray printing and post-processing (http://microcribi.cribi.unipd.it/). cDNA probes were spotted on glass slides across an area of 18 x 18 mm, using a Genpak Array 21 robotic system (Genetix, Hampshire, United Kingdom) equipped with 16 Stealth Micro Spotting Pins SMP 3B (ArrayIt): spots have an average diameter of 120 µm, the distance between two spots is 180 µm, each cDNA probe is spotted in four replicates. GENE ANNOTATION: The cDNA sequences were aligned on the mouse genome (NCBI Build 32) at the UCSC Genome Browser, using the BLAT software. Known genes are described as LocusLink entry; cDNAs of uncertain origin are marked as ‘SIMILAR TO’ or ‘UNKNOWN’, depending if they have similarity or not to other expressed sequences in GenBank. We realized that 8.4% of cDNA probes are possibly redundant, due to multiple isoforms, alternative mRNA terminations, or to cDNA covering different sequences of the same transcript. |
| 11 |
GPL1487 |
BMAP_HGPD |
11,136 |
Canadian Science Centre for Human and Animal Health |
2004-10-09 |
|
Mus musculus
|
cDNA Array |
spotted DNA/cDNA, BMAP_HGPD, Libraries and clones derived from brain regions of adult mouse (strain c57BL6) were obtained from Research Genetics, Inc. The 11,136 clones represent expressed sequence tags (ESTs) from some 5,471 unique known genes and 5,665 mRNAs as yet uncharacterised, and form part of a library created as part of the Brain Molecular Anatomy Project (BMAP). Each insert from this BMAP 3 EST library was amplified by PCR. The cDNA was purified using Millipore multiwell purification plates (Millipore Inc.), and lyophilised PCR products were resuspended in 1 X Micro Spotting Solution Plus (TeleChem International, Inc.) at a concentration of 0.25-0.75 µg/µl. DNA was spotted onto CMT-GAPS coated glass slides (Corning) using Stealth micro-spotting pins (TeleChem International, Inc.). |
| 12 |
GPL1698 |
AECOM Mouse 27k cDNA array |
28,704 |
Albert Einstein College of Medicine |
2004-11-09 |
|
Mus musculus
|
cDNA Array |
spotted DNA/cDNA, AECOM Mouse 27k cDNA array, These cDNA microarrays were obtained from the Albert Einstein College of Medicine Functional Genomics Facility. Each array was spotted in staggered pattern (48 blocks) with 27400 sequence verified mouse clones and 192 bacterial controls on Corning glass slides. 8968 clones were in MGEM1.0 plates from Incyte, 14208 clones in MNCI-V1 plates from NCI (http://lgsun.grc.nia.nih.gov/cDNA/15k.html ) and 4224 clones in MIMP-V1 plates from Research Genetics. Gene annotation was performed at 01/13/2004 using http://genome-www5.stanford.edu/cgi-bin/source//sourceBatchSearch. |
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