| 1 |
GSM45178 |
ALS: SOD1 mouse model, hybridisation 1 |
20,636 |
University of Goettingen |
2005-03-11 |
[cDNA Array] University of Goettingen TAL UniGene_Set_RZPD2_mm2 pattern 06102003 (GPL1800) |
RNA |
Mus musculus
 |
spine |
hSOD1(G93A) transgenic mice: transgenic mice expressing the human SOD1 gene with a G93A mutation (Gurney et al., 1994 [PMID: 8209258]), purchased from The Jackson Laboratory (B6SJL-Tg(SOD1-G93A)1Gur/J, stock no. 002726 [http://www.jax.org/]); fALS (familial amyotrophic lateral sclerosis) mouse model. Control mice: non-transgenic littermates. Timepoint: 17 week-old mice; transgenic mice showed severe signs of paralysis. RNA preparation: isolation of total RNA from spinal cords using RNeasy Maxi Kit (Qiagen) followed by DNase I digestion. RNA processing: RNA amplification (BD Atlas SMART Fluorescent Probe Amplification Kit, starting material: 1000 ng of total RNA, optimised amplification for each sample, stopped at PCR-cycle 18, 19 or 20 within the exponential amplification phase), labeling of amplified cDNA with cy3-/cy5-dCTP (Qiagen LabelStar Array Kit, per array and dye, 1000 ng of amplified cDNA were labeled using two labeling-reactions from the kit). Microarray hybridisation and washing: according to the slide manufacturer´s recommendations (cf. GLP1800); microarray scanning: GMS 428 Microarray Scanner (AffyMetrix). Microarray raw data: software package AIM (Katzer et al., 2003 [PMID: 15376910]). Signals with low quality were excluded from data analysis. Normalisation: pin-wise lowess-regression based normalisation (Yang et al., 2002 [PMID: 11842121]). Keywords = amyotrophic lateral sclerosis, ALS, SOD1 mouse model |
| 2 |
GSM45179 |
ALS: SOD1 mouse model, hybridisation 2 |
20,636 |
University of Goettingen |
2005-03-11 |
[cDNA Array] University of Goettingen TAL UniGene_Set_RZPD2_mm2 pattern 06102003 (GPL1800) |
RNA |
Mus musculus
 |
spine |
hSOD1(G93A) transgenic mice: transgenic mice expressing the human SOD1 gene with a G93A mutation (Gurney et al., 1994 [PMID: 8209258]), purchased from The Jackson Laboratory (B6SJL-Tg(SOD1-G93A)1Gur/J, stock no. 002726 [http://www.jax.org/]); fALS (familial amyotrophic lateral sclerosis) mouse model. Control mice: non-transgenic littermates. Timepoint: 17 week-old mice; transgenic mice showed severe signs of paralysis. RNA preparation: isolation of total RNA from spinal cords using RNeasy Maxi Kit (Qiagen) followed by DNase I digestion. RNA processing: RNA amplification (BD Atlas SMART Fluorescent Probe Amplification Kit, starting material: 1000 ng of total RNA, optimised amplification for each sample, stopped at PCR-cycle 18, 19 or 20 within the exponential amplification phase), labeling of amplified cDNA with cy3-/cy5-dCTP (Qiagen LabelStar Array Kit, per array and dye, 1000 ng of amplified cDNA were labeled using two labeling-reactions from the kit). Microarray hybridisation and washing: according to the slide manufacturer´s recommendations (cf. GLP1800); microarray scanning: GMS 428 Microarray Scanner (AffyMetrix). Microarray raw data: software package AIM (Katzer et al., 2003 [PMID: 15376910]). Signals with low quality were excluded from data analysis. Normalisation: pin-wise lowess-regression based normalisation (Yang et al., 2002 [PMID: 11842121]). Keywords = amyotrophic lateral sclerosis, ALS, SOD1 mouse model |
| 3 |
GSM45180 |
ALS: SOD1 mouse model, hybridisation 3 |
20,636 |
University of Goettingen |
2005-03-11 |
[cDNA Array] University of Goettingen TAL UniGene_Set_RZPD2_mm2 pattern 06102003 (GPL1800) |
RNA |
Mus musculus
 |
spine |
hSOD1(G93A) transgenic mice: transgenic mice expressing the human SOD1 gene with a G93A mutation (Gurney et al., 1994 [PMID: 8209258]), purchased from The Jackson Laboratory (B6SJL-Tg(SOD1-G93A)1Gur/J, stock no. 002726 [http://www.jax.org/]); fALS (familial amyotrophic lateral sclerosis) mouse model. Control mice: non-transgenic littermates. Timepoint: 17 week-old mice; transgenic mice showed severe signs of paralysis. RNA preparation: isolation of total RNA from spinal cords using RNeasy Maxi Kit (Qiagen) followed by DNase I digestion. RNA processing: RNA amplification (BD Atlas SMART Fluorescent Probe Amplification Kit, starting material: 1000 ng of total RNA, optimised amplification for each sample, stopped at PCR-cycle 18, 19 or 20 within the exponential amplification phase), labeling of amplified cDNA with cy3-/cy5-dCTP (Qiagen LabelStar Array Kit, per array and dye, 1000 ng of amplified cDNA were labeled using two labeling-reactions from the kit). Microarray hybridisation and washing: according to the slide manufacturer´s recommendations (cf. GLP1800); microarray scanning: GMS 428 Microarray Scanner (AffyMetrix). Microarray raw data: software package AIM (Katzer et al., 2003 [PMID: 15376910]). Signals with low quality were excluded from data analysis. Normalisation: pin-wise lowess-regression based normalisation (Yang et al., 2002 [PMID: 11842121]). Keywords = amyotrophic lateral sclerosis, ALS, SOD1 mouse model |
| 4 |
GSM45181 |
ALS: SOD1 mouse model, hybridisation 4 |
20,636 |
University of Goettingen |
2005-03-11 |
[cDNA Array] University of Goettingen TAL UniGene_Set_RZPD2_mm2 pattern 06102003 (GPL1800) |
RNA |
Mus musculus
 |
spine |
hSOD1(G93A) transgenic mice: transgenic mice expressing the human SOD1 gene with a G93A mutation (Gurney et al., 1994 [PMID: 8209258]), purchased from The Jackson Laboratory (B6SJL-Tg(SOD1-G93A)1Gur/J, stock no. 002726 [http://www.jax.org/]); fALS (familial amyotrophic lateral sclerosis) mouse model. Control mice: non-transgenic littermates. Timepoint: 17 week-old mice; transgenic mice showed severe signs of paralysis. RNA preparation: isolation of total RNA from spinal cords using RNeasy Maxi Kit (Qiagen) followed by DNase I digestion. RNA processing: RNA amplification (BD Atlas SMART Fluorescent Probe Amplification Kit, starting material: 1000 ng of total RNA, optimised amplification for each sample, stopped at PCR-cycle 18, 19 or 20 within the exponential amplification phase), labeling of amplified cDNA with cy3-/cy5-dCTP (Qiagen LabelStar Array Kit, per array and dye, 1000 ng of amplified cDNA were labeled using two labeling-reactions from the kit). Microarray hybridisation and washing: according to the slide manufacturer´s recommendations (cf. GLP1800); microarray scanning: GMS 428 Microarray Scanner (AffyMetrix). Microarray raw data: software package AIM (Katzer et al., 2003 [PMID: 15376910]). Signals with low quality were excluded from data analysis. Normalisation: pin-wise lowess-regression based normalisation (Yang et al., 2002 [PMID: 11842121]). Keywords = amyotrophic lateral sclerosis, ALS, SOD1 mouse model |
| 5 |
GSM45182 |
ALS: SOD1 mouse model, hybridisation 5 |
20,636 |
University of Goettingen |
2005-03-11 |
[cDNA Array] University of Goettingen TAL UniGene_Set_RZPD2_mm2 pattern 06102003 (GPL1800) |
RNA |
Mus musculus
 |
spine |
hSOD1(G93A) transgenic mice: transgenic mice expressing the human SOD1 gene with a G93A mutation (Gurney et al., 1994 [PMID: 8209258]), purchased from The Jackson Laboratory (B6SJL-Tg(SOD1-G93A)1Gur/J, stock no. 002726 [http://www.jax.org/]); fALS (familial amyotrophic lateral sclerosis) mouse model. Control mice: non-transgenic littermates. Timepoint: 17 week-old mice; transgenic mice showed severe signs of paralysis. RNA preparation: isolation of total RNA from spinal cords using RNeasy Maxi Kit (Qiagen) followed by DNase I digestion. RNA processing: RNA amplification (BD Atlas SMART Fluorescent Probe Amplification Kit, starting material: 1000 ng of total RNA, optimised amplification for each sample, stopped at PCR-cycle 18, 19 or 20 within the exponential amplification phase), labeling of amplified cDNA with cy3-/cy5-dCTP (Qiagen LabelStar Array Kit, per array and dye, 1000 ng of amplified cDNA were labeled using two labeling-reactions from the kit). Microarray hybridisation and washing: according to the slide manufacturer´s recommendations (cf. GLP1800); microarray scanning: GMS 428 Microarray Scanner (AffyMetrix). Microarray raw data: software package AIM (Katzer et al., 2003 [PMID: 15376910]). Signals with low quality were excluded from data analysis. Normalisation: pin-wise lowess-regression based normalisation (Yang et al., 2002 [PMID: 11842121]). Keywords = amyotrophic lateral sclerosis, ALS, SOD1 mouse model |
| 6 |
GSM45183 |
ALS: SOD1 mouse model, hybridisation 6 |
20,636 |
University of Goettingen |
2005-03-11 |
[cDNA Array] University of Goettingen TAL UniGene_Set_RZPD2_mm2 pattern 06102003 (GPL1800) |
RNA |
Mus musculus
 |
spine |
hSOD1(G93A) transgenic mice: transgenic mice expressing the human SOD1 gene with a G93A mutation (Gurney et al., 1994 [PMID: 8209258]), purchased from The Jackson Laboratory (B6SJL-Tg(SOD1-G93A)1Gur/J, stock no. 002726 [http://www.jax.org/]); fALS (familial amyotrophic lateral sclerosis) mouse model. Control mice: non-transgenic littermates. Timepoint: 17 week-old mice; transgenic mice showed severe signs of paralysis. RNA preparation: isolation of total RNA from spinal cords using RNeasy Maxi Kit (Qiagen) followed by DNase I digestion. RNA processing: RNA amplification (BD Atlas SMART Fluorescent Probe Amplification Kit, starting material: 1000 ng of total RNA, optimised amplification for each sample, stopped at PCR-cycle 18, 19 or 20 within the exponential amplification phase), labeling of amplified cDNA with cy3-/cy5-dCTP (Qiagen LabelStar Array Kit, per array and dye, 1000 ng of amplified cDNA were labeled using two labeling-reactions from the kit). Microarray hybridisation and washing: according to the slide manufacturer´s recommendations (cf. GLP1800); microarray scanning: GMS 428 Microarray Scanner (AffyMetrix). Microarray raw data: software package AIM (Katzer et al., 2003 [PMID: 15376910]). Signals with low quality were excluded from data analysis. Normalisation: pin-wise lowess-regression based normalisation (Yang et al., 2002 [PMID: 11842121]). Keywords = amyotrophic lateral sclerosis, ALS, SOD1 mouse model |
| 7 |
GSM437402 |
Group 1 P01 |
223 |
University Medical Center Hamburg-Eppendorf |
2009-08-06 |
[cDNA Array] Eppendorf DualChip Mouse inflammation (GPL8961) |
RNA |
Mus musculus
 |
spleen |
C57Bl/6 mouse PLECs C57Bl/6 mouse spleen |
| 8 |
GSM437403 |
Group 1 P02 |
223 |
University Medical Center Hamburg-Eppendorf |
2009-08-06 |
[cDNA Array] Eppendorf DualChip Mouse inflammation (GPL8961) |
RNA |
Mus musculus
 |
spleen |
C57Bl/6 mouse PLECs C57Bl/6 mouse spleen |
| 9 |
GSM437404 |
Group 1 P03 |
223 |
University Medical Center Hamburg-Eppendorf |
2009-08-06 |
[cDNA Array] Eppendorf DualChip Mouse inflammation (GPL8961) |
RNA |
Mus musculus
 |
spleen |
C57Bl/6 mouse PLECs C57Bl/6 mouse spleen |
| 10 |
GSM437405 |
Group 2 P04 |
223 |
University Medical Center Hamburg-Eppendorf |
2009-08-06 |
[cDNA Array] Eppendorf DualChip Mouse inflammation (GPL8961) |
RNA |
Mus musculus
 |
spleen |
C57Bl/6 mouse PLECs C57Bl/6 mouse spleen |
| 11 |
GSM437406 |
Group 2 P05 |
223 |
University Medical Center Hamburg-Eppendorf |
2009-08-06 |
[cDNA Array] Eppendorf DualChip Mouse inflammation (GPL8961) |
RNA |
Mus musculus
 |
spleen |
C57Bl/6 mouse PLECs C57Bl/6 mouse spleen |
| 12 |
GSM437407 |
Group 2 P06 |
223 |
University Medical Center Hamburg-Eppendorf |
2009-08-06 |
[cDNA Array] Eppendorf DualChip Mouse inflammation (GPL8961) |
RNA |
Mus musculus
 |
spleen |
C57Bl/6 mouse PLECs C57Bl/6 mouse spleen |
| 13 |
GSM437408 |
Group 3 P07 |
223 |
University Medical Center Hamburg-Eppendorf |
2009-08-06 |
[cDNA Array] Eppendorf DualChip Mouse inflammation (GPL8961) |
RNA |
Mus musculus
 |
spleen |
C57Bl/6 mouse PLECs C57Bl/6 mouse spleen |
| 14 |
GSM437409 |
Group 3 P08 |
223 |
University Medical Center Hamburg-Eppendorf |
2009-08-06 |
[cDNA Array] Eppendorf DualChip Mouse inflammation (GPL8961) |
RNA |
Mus musculus
 |
spleen |
C57Bl/6 mouse PLECs C57Bl/6 mouse spleen |
| 15 |
GSM437410 |
Group 3 P09 |
223 |
University Medical Center Hamburg-Eppendorf |
2009-08-06 |
[cDNA Array] Eppendorf DualChip Mouse inflammation (GPL8961) |
RNA |
Mus musculus
 |
spleen |
C57Bl/6 mouse PLECs C57Bl/6 mouse spleen |
| 16 |
GSM171109 |
N2a_22L vers Mock_rep1 |
20,355 |
TU München |
2007-02-23 |
[cDNA Array] GSF/IEG mouse array (GPL3697) |
RNA |
Mus musculus
 |
unclassified |
source_name:N2a_22L N2a_Mock title:N2a_22L vers Mock_rep1 description:RNA isolation, Labelling, Hybridisation and Scanning procedure are described in the fields above |
| 17 |
GSM171110 |
N2a_22L vers Mock_rep2 |
20,355 |
TU München |
2007-02-23 |
[cDNA Array] GSF/IEG mouse array (GPL3697) |
RNA |
Mus musculus
 |
unclassified |
source_name:N2a_22L N2a_Mock title:N2a_22L vers Mock_rep2 description:RNA isolation, Labelling, Hybridisation and Scanning procedure are described in the fields above |
| 18 |
GSM171111 |
N2a_22L vers Mock_rep3 |
20,355 |
TU München |
2007-02-23 |
[cDNA Array] GSF/IEG mouse array (GPL3697) |
RNA |
Mus musculus
 |
unclassified |
source_name:N2a_22L N2a_Mock title:N2a_22L vers Mock_rep3 description:RNA isolation, Labelling, Hybridisation and Scanning procedure are described in the fields above |
| 19 |
GSM171112 |
N2a_22L vers Mock_rep4 |
20,355 |
TU München |
2007-02-23 |
[cDNA Array] GSF/IEG mouse array (GPL3697) |
RNA |
Mus musculus
 |
unclassified |
source_name:N2a_22L N2a_Mock title:N2a_22L vers Mock_rep4 description:RNA isolation, Labelling, Hybridisation and Scanning procedure are described in the fields above |
| 20 |
GSM171114 |
N2a_22L vers Mock_rep5 |
20,355 |
TU München |
2007-02-23 |
[cDNA Array] GSF/IEG mouse array (GPL3697) |
RNA |
Mus musculus
 |
unclassified |
source_name:N2a_22L N2a_Mock title:N2a_22L vers Mock_rep5 description:RNA isolation, Labelling, Hybridisation and Scanning procedure are described in the fields above |