Gene Expression Omnibus (GEO) Overview Version:2014-04-12Japanese page
An overview of the GEO entries broken down by the measurement platforms and the features of the measured samples.
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Data Unit : [ DataSet / Sample / Platform ] Show explanation>> <<Hide explanation
DataSet : Series(GSE) x Platform(GPL). A set of related gene expression data.
Sample : Biological materials.
platform : Methods or instruments used for the gene expression profilings.
The numbers shown in the tabs are the numbers of the data (series, samples or platforms) belonging to the groups.
  Human
(615,734)
  Primates
(5,814)
  Rodents
(225,055)
  Mammals
(20,968)
  Vertebrates
(22,581)
  Invertebrates
(46,584)
  Plants
(107,706)
  Bacteria
(45,091)
  Viruses
(1,432)
  Phages
(112)
  Unclassified
(6,986)
  All
(1,101,311)
 
  SAGE NlaIII
(4)
  SAGE RsaI
(0)
  SAGE Sau3A
(0)
  MPSS
(0)
  GeneChip
(637)
  Tiling Array
(86)
  cDNA Array
(1,377)
  Oligo Array
(3,118)
  Bead Array
(576)
  Protein Array
(0)
  Antibody
(0)
  RT-PCR
(0)
  HT-Seq
(1,091)
  Other
(85)
  All
(6,986)
 
  brain
(173)
  blood
(611)
  connective
(248)
  reproductive
(13)
  muscular
(109)
  digestive
(596)
  liver
(178)
  lung
(73)
  urinary
(42)
  endo/exo-crine
(127)
  embryo
(30)
  adult aerial structure
(0)
  young aerial structure
(0)
  root
(6)
  meristem/growing tissue
(0)
  flower/sexual organ
(0)
  seed/fruit/grain
(6)
  pooled
(439)
  unclassified
(4,335)
  all
(6,986)
 
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Sample ID Title Number of Data Institute Submission date Platform Sample type Species Organ class Reasoning of the classification
Keywords used for the classification are shown with bold font.
1 GSM635505 P7.5A control 2,352 Universidade de São Paulo 2010-12-07 [cDNA Array] HAC Human 2.3K (GPL9692) RNA Mus musculus,Homo sapiens
Mus musculus,Homo sapiens
kidney This research was approved by Ethic Committee of the Medical and Research Center - Hospital A. C. Camargo number 764/06. Twenty-four sporadic and favorable histology advanced (Stage III or IV) Wilms tumor (WT) samples from Children Oncology Group (NWTSG), four human differentiated kidneys , and four temporal stages of kidney differentiation in mouse (E15.5, E17.5, P1.5 and P7.5) were used for this study. Metanephric blastema cells from four temporal differentiation stages, from E15.5 to the fully differentiated kidney in mouse, and blastemal cells of WT and differentiated kidneys in human were used for RNA extraction and amplification. RNA were extracted with PicoPureâ„¢ RNA Isolation kit (Arcturus Engineering #KT0204) and treated with RNase-Free DNase Set (Qiagen #79254; Qiagen-Germantown MD USA). Amplification methodology used RiboAmpTM RNA amplification KIT0201 and RiboAmpTM HS Plus KIT0505 (ARCTURUS) for human and mouse samples, respectively. A pool of RNAs obtained from 15 distinct human cell lines was amplified in the presence of SuperScript III and oligo dT(24)-T7. Amplified RNA was then used in a transcriptase reverse reaction into cDNA in the presence of Cy3 or Cy5-labeled dCTP. Dye-swap was performed for each sample as control for dye bias and used as replicate.
2 GSM635506 P7.5A swap 2,352 Universidade de São Paulo 2010-12-07 [cDNA Array] HAC Human 2.3K (GPL9692) RNA Homo sapiens,Mus musculus
Homo sapiens,Mus musculus
kidney This research was approved by Ethic Committee of the Medical and Research Center - Hospital A. C. Camargo number 764/06. Twenty-four sporadic and favorable histology advanced (Stage III or IV) Wilms tumor (WT) samples from Children Oncology Group (NWTSG), four human differentiated kidneys , and four temporal stages of kidney differentiation in mouse (E15.5, E17.5, P1.5 and P7.5) were used for this study. Metanephric blastema cells from four temporal differentiation stages, from E15.5 to the fully differentiated kidney in mouse, and blastemal cells of WT and differentiated kidneys in human were used for RNA extraction and amplification. RNA were extracted with PicoPureâ„¢ RNA Isolation kit (Arcturus Engineering #KT0204) and treated with RNase-Free DNase Set (Qiagen #79254; Qiagen-Germantown MD USA). Amplification methodology used RiboAmpTM RNA amplification KIT0201 and RiboAmpTM HS Plus KIT0505 (ARCTURUS) for human and mouse samples, respectively. A pool of RNAs obtained from 15 distinct human cell lines was amplified in the presence of SuperScript III and oligo dT(24)-T7. Amplified RNA was then used in a transcriptase reverse reaction into cDNA in the presence of Cy3 or Cy5-labeled dCTP. Dye-swap was performed for each sample as control for dye bias and used as replicate.
3 GSM635559 E15.5G control 2,352 Universidade de São Paulo 2010-12-07 [cDNA Array] HAC Human 2.3K (GPL9692) RNA Mus musculus,Homo sapiens
Mus musculus,Homo sapiens
kidney This research was approved by Ethic Committee of the Medical and Research Center - Hospital A. C. Camargo number 764/06. Twenty-four sporadic and favorable histology advanced (Stage III or IV) Wilms tumor (WT) samples from Children Oncology Group (NWTSG), four human differentiated kidneys , and four temporal stages of kidney differentiation in mouse (E15.5, E17.5, P1.5 and P7.5) were used for this study. Metanephric blastema cells from four temporal differentiation stages, from E15.5 to the fully differentiated kidney in mouse, and blastemal cells of WT and differentiated kidneys in human were used for RNA extraction and amplification. RNA were extracted with PicoPureâ„¢ RNA Isolation kit (Arcturus Engineering #KT0204) and treated with RNase-Free DNase Set (Qiagen #79254; Qiagen-Germantown MD USA). Amplification methodology used RiboAmpTM RNA amplification KIT0201 and RiboAmpTM HS Plus KIT0505 (ARCTURUS) for human and mouse samples, respectively. A pool of RNAs obtained from 15 distinct human cell lines was amplified in the presence of SuperScript III and oligo dT(24)-T7. Amplified RNA was then used in a transcriptase reverse reaction into cDNA in the presence of Cy3 or Cy5-labeled dCTP. Dye-swap was performed for each sample as control for dye bias and used as replicate.
4 GSM635560 E15.5G swap 2,352 Universidade de São Paulo 2010-12-07 [cDNA Array] HAC Human 2.3K (GPL9692) RNA Homo sapiens,Mus musculus
Homo sapiens,Mus musculus
kidney This research was approved by Ethic Committee of the Medical and Research Center - Hospital A. C. Camargo number 764/06. Twenty-four sporadic and favorable histology advanced (Stage III or IV) Wilms tumor (WT) samples from Children Oncology Group (NWTSG), four human differentiated kidneys , and four temporal stages of kidney differentiation in mouse (E15.5, E17.5, P1.5 and P7.5) were used for this study. Metanephric blastema cells from four temporal differentiation stages, from E15.5 to the fully differentiated kidney in mouse, and blastemal cells of WT and differentiated kidneys in human were used for RNA extraction and amplification. RNA were extracted with PicoPureâ„¢ RNA Isolation kit (Arcturus Engineering #KT0204) and treated with RNase-Free DNase Set (Qiagen #79254; Qiagen-Germantown MD USA). Amplification methodology used RiboAmpTM RNA amplification KIT0201 and RiboAmpTM HS Plus KIT0505 (ARCTURUS) for human and mouse samples, respectively. A pool of RNAs obtained from 15 distinct human cell lines was amplified in the presence of SuperScript III and oligo dT(24)-T7. Amplified RNA was then used in a transcriptase reverse reaction into cDNA in the presence of Cy3 or Cy5-labeled dCTP. Dye-swap was performed for each sample as control for dye bias and used as replicate.
5 GSM635561 E17.5B control 2,352 Universidade de São Paulo 2010-12-07 [cDNA Array] HAC Human 2.3K (GPL9692) RNA Mus musculus,Homo sapiens
Mus musculus,Homo sapiens
kidney This research was approved by Ethic Committee of the Medical and Research Center - Hospital A. C. Camargo number 764/06. Twenty-four sporadic and favorable histology advanced (Stage III or IV) Wilms tumor (WT) samples from Children Oncology Group (NWTSG), four human differentiated kidneys , and four temporal stages of kidney differentiation in mouse (E15.5, E17.5, P1.5 and P7.5) were used for this study. Metanephric blastema cells from four temporal differentiation stages, from E15.5 to the fully differentiated kidney in mouse, and blastemal cells of WT and differentiated kidneys in human were used for RNA extraction and amplification. RNA were extracted with PicoPureâ„¢ RNA Isolation kit (Arcturus Engineering #KT0204) and treated with RNase-Free DNase Set (Qiagen #79254; Qiagen-Germantown MD USA). Amplification methodology used RiboAmpTM RNA amplification KIT0201 and RiboAmpTM HS Plus KIT0505 (ARCTURUS) for human and mouse samples, respectively. A pool of RNAs obtained from 15 distinct human cell lines was amplified in the presence of SuperScript III and oligo dT(24)-T7. Amplified RNA was then used in a transcriptase reverse reaction into cDNA in the presence of Cy3 or Cy5-labeled dCTP. Dye-swap was performed for each sample as control for dye bias and used as replicate.
6 GSM635562 E17.5B swap 2,352 Universidade de São Paulo 2010-12-07 [cDNA Array] HAC Human 2.3K (GPL9692) RNA Homo sapiens,Mus musculus
Homo sapiens,Mus musculus
kidney This research was approved by Ethic Committee of the Medical and Research Center - Hospital A. C. Camargo number 764/06. Twenty-four sporadic and favorable histology advanced (Stage III or IV) Wilms tumor (WT) samples from Children Oncology Group (NWTSG), four human differentiated kidneys , and four temporal stages of kidney differentiation in mouse (E15.5, E17.5, P1.5 and P7.5) were used for this study. Metanephric blastema cells from four temporal differentiation stages, from E15.5 to the fully differentiated kidney in mouse, and blastemal cells of WT and differentiated kidneys in human were used for RNA extraction and amplification. RNA were extracted with PicoPureâ„¢ RNA Isolation kit (Arcturus Engineering #KT0204) and treated with RNase-Free DNase Set (Qiagen #79254; Qiagen-Germantown MD USA). Amplification methodology used RiboAmpTM RNA amplification KIT0201 and RiboAmpTM HS Plus KIT0505 (ARCTURUS) for human and mouse samples, respectively. A pool of RNAs obtained from 15 distinct human cell lines was amplified in the presence of SuperScript III and oligo dT(24)-T7. Amplified RNA was then used in a transcriptase reverse reaction into cDNA in the presence of Cy3 or Cy5-labeled dCTP. Dye-swap was performed for each sample as control for dye bias and used as replicate.
7 GSM635563 E17.5D control 2,352 Universidade de São Paulo 2010-12-07 [cDNA Array] HAC Human 2.3K (GPL9692) RNA Mus musculus,Homo sapiens
Mus musculus,Homo sapiens
kidney This research was approved by Ethic Committee of the Medical and Research Center - Hospital A. C. Camargo number 764/06. Twenty-four sporadic and favorable histology advanced (Stage III or IV) Wilms tumor (WT) samples from Children Oncology Group (NWTSG), four human differentiated kidneys , and four temporal stages of kidney differentiation in mouse (E15.5, E17.5, P1.5 and P7.5) were used for this study. Metanephric blastema cells from four temporal differentiation stages, from E15.5 to the fully differentiated kidney in mouse, and blastemal cells of WT and differentiated kidneys in human were used for RNA extraction and amplification. RNA were extracted with PicoPureâ„¢ RNA Isolation kit (Arcturus Engineering #KT0204) and treated with RNase-Free DNase Set (Qiagen #79254; Qiagen-Germantown MD USA). Amplification methodology used RiboAmpTM RNA amplification KIT0201 and RiboAmpTM HS Plus KIT0505 (ARCTURUS) for human and mouse samples, respectively. A pool of RNAs obtained from 15 distinct human cell lines was amplified in the presence of SuperScript III and oligo dT(24)-T7. Amplified RNA was then used in a transcriptase reverse reaction into cDNA in the presence of Cy3 or Cy5-labeled dCTP. Dye-swap was performed for each sample as control for dye bias and used as replicate.
8 GSM635564 E17.5D swap 2,352 Universidade de São Paulo 2010-12-07 [cDNA Array] HAC Human 2.3K (GPL9692) RNA Homo sapiens,Mus musculus
Homo sapiens,Mus musculus
kidney This research was approved by Ethic Committee of the Medical and Research Center - Hospital A. C. Camargo number 764/06. Twenty-four sporadic and favorable histology advanced (Stage III or IV) Wilms tumor (WT) samples from Children Oncology Group (NWTSG), four human differentiated kidneys , and four temporal stages of kidney differentiation in mouse (E15.5, E17.5, P1.5 and P7.5) were used for this study. Metanephric blastema cells from four temporal differentiation stages, from E15.5 to the fully differentiated kidney in mouse, and blastemal cells of WT and differentiated kidneys in human were used for RNA extraction and amplification. RNA were extracted with PicoPureâ„¢ RNA Isolation kit (Arcturus Engineering #KT0204) and treated with RNase-Free DNase Set (Qiagen #79254; Qiagen-Germantown MD USA). Amplification methodology used RiboAmpTM RNA amplification KIT0201 and RiboAmpTM HS Plus KIT0505 (ARCTURUS) for human and mouse samples, respectively. A pool of RNAs obtained from 15 distinct human cell lines was amplified in the presence of SuperScript III and oligo dT(24)-T7. Amplified RNA was then used in a transcriptase reverse reaction into cDNA in the presence of Cy3 or Cy5-labeled dCTP. Dye-swap was performed for each sample as control for dye bias and used as replicate.
9 GSM635565 E17.5C 2,352 Universidade de São Paulo 2010-12-07 [cDNA Array] HAC Human 2.3K (GPL9692) RNA Mus musculus,Homo sapiens
Mus musculus,Homo sapiens
kidney This research was approved by Ethic Committee of the Medical and Research Center - Hospital A. C. Camargo number 764/06. Twenty-four sporadic and favorable histology advanced (Stage III or IV) Wilms tumor (WT) samples from Children Oncology Group (NWTSG), four human differentiated kidneys , and four temporal stages of kidney differentiation in mouse (E15.5, E17.5, P1.5 and P7.5) were used for this study. Metanephric blastema cells from four temporal differentiation stages, from E15.5 to the fully differentiated kidney in mouse, and blastemal cells of WT and differentiated kidneys in human were used for RNA extraction and amplification. RNA were extracted with PicoPureâ„¢ RNA Isolation kit (Arcturus Engineering #KT0204) and treated with RNase-Free DNase Set (Qiagen #79254; Qiagen-Germantown MD USA). Amplification methodology used RiboAmpTM RNA amplification KIT0201 and RiboAmpTM HS Plus KIT0505 (ARCTURUS) for human and mouse samples, respectively. A pool of RNAs obtained from 15 distinct human cell lines was amplified in the presence of SuperScript III and oligo dT(24)-T7. Amplified RNA was then used in a transcriptase reverse reaction into cDNA in the presence of Cy3 or Cy5-labeled dCTP. Dye-swap was performed for each sample as control for dye bias and used as replicate.
10 GSM635566 E17.5C swap 2,352 Universidade de São Paulo 2010-12-07 [cDNA Array] HAC Human 2.3K (GPL9692) RNA Homo sapiens,Mus musculus
Homo sapiens,Mus musculus
kidney This research was approved by Ethic Committee of the Medical and Research Center - Hospital A. C. Camargo number 764/06. Twenty-four sporadic and favorable histology advanced (Stage III or IV) Wilms tumor (WT) samples from Children Oncology Group (NWTSG), four human differentiated kidneys , and four temporal stages of kidney differentiation in mouse (E15.5, E17.5, P1.5 and P7.5) were used for this study. Metanephric blastema cells from four temporal differentiation stages, from E15.5 to the fully differentiated kidney in mouse, and blastemal cells of WT and differentiated kidneys in human were used for RNA extraction and amplification. RNA were extracted with PicoPureâ„¢ RNA Isolation kit (Arcturus Engineering #KT0204) and treated with RNase-Free DNase Set (Qiagen #79254; Qiagen-Germantown MD USA). Amplification methodology used RiboAmpTM RNA amplification KIT0201 and RiboAmpTM HS Plus KIT0505 (ARCTURUS) for human and mouse samples, respectively. A pool of RNAs obtained from 15 distinct human cell lines was amplified in the presence of SuperScript III and oligo dT(24)-T7. Amplified RNA was then used in a transcriptase reverse reaction into cDNA in the presence of Cy3 or Cy5-labeled dCTP. Dye-swap was performed for each sample as control for dye bias and used as replicate.
11 GSM635577 P1.5A control 2,352 Universidade de São Paulo 2010-12-07 [cDNA Array] HAC Human 2.3K (GPL9692) RNA Mus musculus,Homo sapiens
Mus musculus,Homo sapiens
kidney This research was approved by Ethic Committee of the Medical and Research Center - Hospital A. C. Camargo number 764/06. Twenty-four sporadic and favorable histology advanced (Stage III or IV) Wilms tumor (WT) samples from Children Oncology Group (NWTSG), four human differentiated kidneys , and four temporal stages of kidney differentiation in mouse (E15.5, E17.5, P1.5 and P7.5) were used for this study. Metanephric blastema cells from four temporal differentiation stages, from E15.5 to the fully differentiated kidney in mouse, and blastemal cells of WT and differentiated kidneys in human were used for RNA extraction and amplification. RNA were extracted with PicoPureâ„¢ RNA Isolation kit (Arcturus Engineering #KT0204) and treated with RNase-Free DNase Set (Qiagen #79254; Qiagen-Germantown MD USA). Amplification methodology used RiboAmpTM RNA amplification KIT0201 and RiboAmpTM HS Plus KIT0505 (ARCTURUS) for human and mouse samples, respectively. A pool of RNAs obtained from 15 distinct human cell lines was amplified in the presence of SuperScript III and oligo dT(24)-T7. Amplified RNA was then used in a transcriptase reverse reaction into cDNA in the presence of Cy3 or Cy5-labeled dCTP. Dye-swap was performed for each sample as control for dye bias and used as replicate.
12 GSM635578 P1.5A swap 2,352 Universidade de São Paulo 2010-12-07 [cDNA Array] HAC Human 2.3K (GPL9692) RNA Homo sapiens,Mus musculus
Homo sapiens,Mus musculus
kidney This research was approved by Ethic Committee of the Medical and Research Center - Hospital A. C. Camargo number 764/06. Twenty-four sporadic and favorable histology advanced (Stage III or IV) Wilms tumor (WT) samples from Children Oncology Group (NWTSG), four human differentiated kidneys , and four temporal stages of kidney differentiation in mouse (E15.5, E17.5, P1.5 and P7.5) were used for this study. Metanephric blastema cells from four temporal differentiation stages, from E15.5 to the fully differentiated kidney in mouse, and blastemal cells of WT and differentiated kidneys in human were used for RNA extraction and amplification. RNA were extracted with PicoPureâ„¢ RNA Isolation kit (Arcturus Engineering #KT0204) and treated with RNase-Free DNase Set (Qiagen #79254; Qiagen-Germantown MD USA). Amplification methodology used RiboAmpTM RNA amplification KIT0201 and RiboAmpTM HS Plus KIT0505 (ARCTURUS) for human and mouse samples, respectively. A pool of RNAs obtained from 15 distinct human cell lines was amplified in the presence of SuperScript III and oligo dT(24)-T7. Amplified RNA was then used in a transcriptase reverse reaction into cDNA in the presence of Cy3 or Cy5-labeled dCTP. Dye-swap was performed for each sample as control for dye bias and used as replicate.
13 GSM635579 P1.5B control 2,352 Universidade de São Paulo 2010-12-07 [cDNA Array] HAC Human 2.3K (GPL9692) RNA Mus musculus,Homo sapiens
Mus musculus,Homo sapiens
kidney This research was approved by Ethic Committee of the Medical and Research Center - Hospital A. C. Camargo number 764/06. Twenty-four sporadic and favorable histology advanced (Stage III or IV) Wilms tumor (WT) samples from Children Oncology Group (NWTSG), four human differentiated kidneys , and four temporal stages of kidney differentiation in mouse (E15.5, E17.5, P1.5 and P7.5) were used for this study. Metanephric blastema cells from four temporal differentiation stages, from E15.5 to the fully differentiated kidney in mouse, and blastemal cells of WT and differentiated kidneys in human were used for RNA extraction and amplification. RNA were extracted with PicoPureâ„¢ RNA Isolation kit (Arcturus Engineering #KT0204) and treated with RNase-Free DNase Set (Qiagen #79254; Qiagen-Germantown MD USA). Amplification methodology used RiboAmpTM RNA amplification KIT0201 and RiboAmpTM HS Plus KIT0505 (ARCTURUS) for human and mouse samples, respectively. A pool of RNAs obtained from 15 distinct human cell lines was amplified in the presence of SuperScript III and oligo dT(24)-T7. Amplified RNA was then used in a transcriptase reverse reaction into cDNA in the presence of Cy3 or Cy5-labeled dCTP. Dye-swap was performed for each sample as control for dye bias and used as replicate.
14 GSM635580 P1.5B swap 2,352 Universidade de São Paulo 2010-12-07 [cDNA Array] HAC Human 2.3K (GPL9692) RNA Homo sapiens,Mus musculus
Homo sapiens,Mus musculus
kidney This research was approved by Ethic Committee of the Medical and Research Center - Hospital A. C. Camargo number 764/06. Twenty-four sporadic and favorable histology advanced (Stage III or IV) Wilms tumor (WT) samples from Children Oncology Group (NWTSG), four human differentiated kidneys , and four temporal stages of kidney differentiation in mouse (E15.5, E17.5, P1.5 and P7.5) were used for this study. Metanephric blastema cells from four temporal differentiation stages, from E15.5 to the fully differentiated kidney in mouse, and blastemal cells of WT and differentiated kidneys in human were used for RNA extraction and amplification. RNA were extracted with PicoPureâ„¢ RNA Isolation kit (Arcturus Engineering #KT0204) and treated with RNase-Free DNase Set (Qiagen #79254; Qiagen-Germantown MD USA). Amplification methodology used RiboAmpTM RNA amplification KIT0201 and RiboAmpTM HS Plus KIT0505 (ARCTURUS) for human and mouse samples, respectively. A pool of RNAs obtained from 15 distinct human cell lines was amplified in the presence of SuperScript III and oligo dT(24)-T7. Amplified RNA was then used in a transcriptase reverse reaction into cDNA in the presence of Cy3 or Cy5-labeled dCTP. Dye-swap was performed for each sample as control for dye bias and used as replicate.
15 GSM635581 P1.5C control 2,352 Universidade de São Paulo 2010-12-07 [cDNA Array] HAC Human 2.3K (GPL9692) RNA Mus musculus,Homo sapiens
Mus musculus,Homo sapiens
kidney This research was approved by Ethic Committee of the Medical and Research Center - Hospital A. C. Camargo number 764/06. Twenty-four sporadic and favorable histology advanced (Stage III or IV) Wilms tumor (WT) samples from Children Oncology Group (NWTSG), four human differentiated kidneys , and four temporal stages of kidney differentiation in mouse (E15.5, E17.5, P1.5 and P7.5) were used for this study. Metanephric blastema cells from four temporal differentiation stages, from E15.5 to the fully differentiated kidney in mouse, and blastemal cells of WT and differentiated kidneys in human were used for RNA extraction and amplification. RNA were extracted with PicoPureâ„¢ RNA Isolation kit (Arcturus Engineering #KT0204) and treated with RNase-Free DNase Set (Qiagen #79254; Qiagen-Germantown MD USA). Amplification methodology used RiboAmpTM RNA amplification KIT0201 and RiboAmpTM HS Plus KIT0505 (ARCTURUS) for human and mouse samples, respectively. A pool of RNAs obtained from 15 distinct human cell lines was amplified in the presence of SuperScript III and oligo dT(24)-T7. Amplified RNA was then used in a transcriptase reverse reaction into cDNA in the presence of Cy3 or Cy5-labeled dCTP. Dye-swap was performed for each sample as control for dye bias and used as replicate.
16 GSM635582 P1.5C swap 2,352 Universidade de São Paulo 2010-12-07 [cDNA Array] HAC Human 2.3K (GPL9692) RNA Homo sapiens,Mus musculus
Homo sapiens,Mus musculus
kidney This research was approved by Ethic Committee of the Medical and Research Center - Hospital A. C. Camargo number 764/06. Twenty-four sporadic and favorable histology advanced (Stage III or IV) Wilms tumor (WT) samples from Children Oncology Group (NWTSG), four human differentiated kidneys , and four temporal stages of kidney differentiation in mouse (E15.5, E17.5, P1.5 and P7.5) were used for this study. Metanephric blastema cells from four temporal differentiation stages, from E15.5 to the fully differentiated kidney in mouse, and blastemal cells of WT and differentiated kidneys in human were used for RNA extraction and amplification. RNA were extracted with PicoPureâ„¢ RNA Isolation kit (Arcturus Engineering #KT0204) and treated with RNase-Free DNase Set (Qiagen #79254; Qiagen-Germantown MD USA). Amplification methodology used RiboAmpTM RNA amplification KIT0201 and RiboAmpTM HS Plus KIT0505 (ARCTURUS) for human and mouse samples, respectively. A pool of RNAs obtained from 15 distinct human cell lines was amplified in the presence of SuperScript III and oligo dT(24)-T7. Amplified RNA was then used in a transcriptase reverse reaction into cDNA in the presence of Cy3 or Cy5-labeled dCTP. Dye-swap was performed for each sample as control for dye bias and used as replicate.
17 GSM1219494 E8 0 University of Edinburgh 2013-08-29 [HT Sequencing] Illumina MiSeq (Homo sapiens; Saccharomyces cerevisiae) (GPL17658) SRA Saccharomyces cerevisiae,Homo sapiens
Saccharomyces cerevisiae,Homo sapiens
kidney Flp-in T-REx 293-PTH-AGO1 + BY4741
18 GSM1219496 E10 0 University of Edinburgh 2013-08-29 [HT Sequencing] Illumina MiSeq (Homo sapiens; Saccharomyces cerevisiae) (GPL17658) SRA Saccharomyces cerevisiae,Homo sapiens
Saccharomyces cerevisiae,Homo sapiens
kidney Flp-in T-REx 293-PTH-AGO1 + BY4741
19 GSM281674 Control replicate 1 480 University of Minnesota 2008-04-14 [Oligo Array] University of Minnesota multi-species miRNA 480 (GPL6741) mixed Homo sapiens,synthetic construct
Homo sapiens,synthetic construct
kidney Total RNA from 293T cells Reference DNA
20 GSM281675 A replicate 1 480 University of Minnesota 2008-04-14 [Oligo Array] University of Minnesota multi-species miRNA 480 (GPL6741) mixed Homo sapiens,synthetic construct
Homo sapiens,synthetic construct
kidney Total RNA from 293T transformed cells A Reference DNA
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