| 1 |
GPL1743 |
Zebrafish nucleotide array |
15,174 |
Leiden University |
2004-12-10 |
|
Danio rerio
 |
Oligo Array |
spotted oligonucleotide, Zebrafish nucleotide array, Microarrays of the MWG 14k Zebrafish Oligonucleotide Set were produced using the facilities of the Leiden Genome Technology Center (http://www.lgtc.nl). Oligonucleotides were dissolved in 150mM phosphate buffer (pH 8.5) to a concentration of 20 µM. With Omnigrid 100 (Genemachines) oligonucleotides were spotted on Codelink activated slides (Amersham Biosciences) according to the Amersham Codelink protocol and as described in (‘t Hoen et al 2003 and Meijer et al 2004). Microarrays were spotted according to the MIAME guidelines with 15.532 unique zebrafish oligonucleotides from the MWG oligonucleotide set. Ambion’s arraycontrol oligonucleotides (8 sense oligos) and 3 custom designed oligonucleotides were spotted together with the MWG oligonucleotide set. Spotted Codelink activated slides were treated with blocking solution and washed according to the manufacturer’s instructions. |
| 2 |
GPL1742 |
UD_Liver_3.2K |
3,456 |
University of Delaware |
2004-12-09 |
|
Gallus gallus
 |
cDNA Array |
spotted DNA/cDNA, UD_Liver_3.2K, Spotted amplified cDNA array on nylon membrane (8 x 12 cm). 3456 spots Keywords = spotted Keywords = cDNA |
| 3 |
GPL1737 |
UD 7.4K Metabolic/Somatic Systems |
7,680 |
University of Delaware |
2004-12-08 |
|
Gallus gallus
|
cDNA Array |
spotted DNA/cDNA, UD 7.4K Metabolic/Somatic Systems, Spotted amplified cDNA array on glass. 7680 spots corresponding to 7435 genes from Liver and Adipose tissue. |
| 4 |
GPL1477 |
SAGE:17:NlaIII:Gallus gallus |
48,341 |
|
2004-10-04 |
|
Gallus gallus
|
SAGE NlaIII |
SAGE NlaIII, SAGE:17:NlaIII:Gallus gallus, This is a virtual platform for longSAGE 17 libraries generated for Gallus gallus using NlaIII as an anchor enzyme. Platform_anchor: NlaIII |
| 5 |
GPL1724 |
SAGE:10:NlaIII:Gallus gallus |
4,090 |
|
2004-11-30 |
|
Gallus gallus
|
SAGE NlaIII |
SAGE NlaIII, SAGE:10:NlaIII:Gallus gallus, This is a virtual platform for SAGE libraries generated for Gallus gallus using NlaIII as an anchor enzyme. Platform_anchor: NlaIII |
| 6 |
GPL1516 |
SA-DEV-VS1 |
21,600 |
HCMR |
2004-10-19 |
|
Sparus aurata
 |
cDNA Array |
spotted DNA/cDNA, SA-DEV-VS1, For the microarray production a cDNA library of mixed embryos/larvae of gilthead sea bream, Sparus aurata (Sarropoulou et al., in press) was used. A total of 10,176 clones were arrayed into 384 square well plates. In order to amplify the inserts culture PCR was performed as followed. The total volume of one PCR reaction was 50 µl, containing 43.5 µl reaction mix (2 µl 10x PCR Puffer, 0.04 µl 100 mM of each dATP, dCTP, dGTP and dTTP, 12.12 µl dH20), 1.5 µl of T7 and 1.5 µl of T3 primer (20 µM), 1 µl Taq polymerase (5U/µl) and 2.5 µl of culture. The PCR program utilized consisted of 1 step of 94ºC for 1 min 20 s, followed by 35 cycles of 94ºC for 1 min, 50ºC for 1 min, 72ºC for 1 min 30 s; the program terminated with a final step of 72ºC for 5min. Each reaction was performed twice; PCR products were combined, separated by agarose gel (2%) electrophoresis and visualized by transillumination in the presence of ethidium bromide (EtBr) in order to check insert size. Purification of PCR products was perfomed via the GFX-96TM Polymerase Chain Reaction (PCR) Purification Kit (Amersham Pharmacia) according to the manufacturer’s instructions. The purified products were then dried down by applying vacuum, re-eluted in 3x SSC and 1.5 M betaine to a volume of 10 ml and transferred to 384 well plates. The spotting was performed with the OmniGrid 300 robotic system configured with 48 pins. Each glass slide contains 10,176 PCR amplified inserts in duplicate. The spotted glass slides were baked at 80ºC for 2 h in order to cross-link the DNA onto the glass slide. The slides were then incubated in blocking buffer at 50°C for 30 min, washed twice with Millipore water at room temperature for 2 min and dried by spinning in an Eppendorf 5810R centrifuge for 7 min at 800 rpm. |
| 7 |
GPL1731 |
DEL-MAR 14K Integrated Systems |
19,200 |
University of Delaware |
2004-12-06 |
|
Gallus gallus
|
cDNA Array |
spotted DNA/cDNA, DEL-MAR 14K Integrated Systems, Spotted amplified cDNA array on glass. 19200 spot corresponding to 14053 different genes. liver / fat / muscle / pituitary / hypothalamus / pineal / testis / ovary / oviduct / Oligo |
| 8 |
GPL1744 |
Chicken_Neuroendocrine_System_5K |
7,200 |
University of Maryland |
2004-12-10 |
|
Gallus gallus
|
cDNA Array |
spotted DNA/cDNA, Chicken_Neuroendocrine_System_5K, Spotted amplified cDNA array on glass. Produced with cDNA clones expressed in the chicken neuroendocrine system. Tissues used to produced the cDNA library included the hypothalamus, anterior pituitary gland, and pineal gland. 7200 spots Keywords = spotted Keywords = cDNA |
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