Gene Expression Omnibus (GEO) Overview

DataSet	:	Series(GSE) x Platform(GPL). A set of related gene expression data.
Sample	:	Biological materials.
platform	:	Methods or instruments used for the gene expression profilings.

All
(898,944)

SAGE NlaIII
(1,683)

SAGE RsaI
(3)

SAGE Sau3A
(54)

MPSS
(439)

GeneChip
(377,145)

Tiling Array
(21,846)

cDNA Array
(103,476)

Oligo Array
(239,584)

Antibody
(1,152)

embryo
(0)

adult aerial structure
(0)

young aerial structure
(0)

root
(0)

meristem/growing tissue
(0)

flower/sexual organ
(0)

seed/fruit/grain
(0)

pooled
(0)

unclassified
(85)

all
(1,152)

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	Sample ID	Title	Number of Data	Institute	Submission date	Platform	Sample type	Species	Organ class	Reasoning of the classification Keywords used for the classification are shown with bold font.
1	GSM11959	slide 1166	20,800	SA Pathology	2003-10-22	[Antibody] angiogenesis (GPL555)	RNA	Homo sapiens	vein	HUVEC from 3 different umbilical cords were plated onto 100ul of bovine type I collagen in 96-well microtitre plates (15 wells/cord). At times 0, 0.5, 3, 6 and 24 hours after stimulation with PMA and RMAC11, total RNA was isolated using the TRIZOL procedure. Message Amp aRNA Kit was used to linearly amplify the mRNA approximately 400-fold. The aRNA was generated incorporating 5-(3-aminoallyl)-UTP and subsequently coupled to Cy3 and Cy5 dyes.
2	GSM11960	slide 1167	20,800	SA Pathology	2003-10-22	[Antibody] angiogenesis (GPL555)	RNA	Homo sapiens	vein	HUVEC from 3 different umbilical cords were plated onto 100ul of bovine type I collagen in 96-well microtitre plates (15 wells/cord). At times 0, 0.5, 3, 6 and 24 hours after stimulation with PMA and RMAC11, total RNA was isolated using the TRIZOL procedure. Message Amp aRNA Kit was used to linearly amplify the mRNA approximately 400-fold. The aRNA was generated incorporating 5-(3-aminoallyl)-UTP and subsequently coupled to Cy3 and Cy5 dyes.
3	GSM11961	slide 1163	20,800	SA Pathology	2003-10-22	[Antibody] angiogenesis (GPL555)	RNA	Homo sapiens	vein	HUVEC from 3 different umbilical cords were plated onto 100ul of bovine type I collagen in 96-well microtitre plates (15 wells/cord). At times 0, 0.5, 3, 6 and 24 hours after stimulation with PMA and RMAC11, total RNA was isolated using the TRIZOL procedure. Message Amp aRNA Kit was used to linearly amplify the mRNA approximately 400-fold. The aRNA was generated incorporating 5-(3-aminoallyl)-UTP and subsequently coupled to Cy3 and Cy5 dyes.
4	GSM11962	slide 1164	20,800	SA Pathology	2003-10-22	[Antibody] angiogenesis (GPL555)	RNA	Homo sapiens	vein	HUVEC from 3 different umbilical cords were plated onto 100ul of bovine type I collagen in 96-well microtitre plates (15 wells/cord). At times 0, 0.5, 3, 6 and 24 hours after stimulation with PMA and RMAC11, total RNA was isolated using the TRIZOL procedure. Message Amp aRNA Kit was used to linearly amplify the mRNA approximately 400-fold. The aRNA was generated incorporating 5-(3-aminoallyl)-UTP and subsequently coupled to Cy3 and Cy5 dyes.
5	GSM12034	slide 1161	20,800	SA Pathology	2003-10-22	[Antibody] angiogenesis (GPL555)	RNA	Homo sapiens	vein	HUVEC from 3 different umbilical cords were plated onto 100ul of bovine type I collagen in 96-well microtitre plates (15 wells/cord). At times 0, 0.5, 3, 6 and 24 hours after stimulation with PMA and RMAC11, total RNA was isolated using the TRIZOL procedure. Message Amp aRNA Kit was used to linearly amplify the mRNA approximately 400-fold. The aRNA was generated incorporating 5-(3-aminoallyl)-UTP and subsequently coupled to Cy3 and Cy5 dyes.
6	GSM12035	slide 1162	20,800	SA Pathology	2003-10-22	[Antibody] angiogenesis (GPL555)	RNA	Homo sapiens	vein	HUVEC from 3 different umbilical cords were plated onto 100ul of bovine type I collagen in 96-well microtitre plates (15 wells/cord). At times 0, 0.5, 3, 6 and 24 hours after stimulation with PMA and RMAC11, total RNA was isolated using the TRIZOL procedure. Message Amp aRNA Kit was used to linearly amplify the mRNA approximately 400-fold. The aRNA was generated incorporating 5-(3-aminoallyl)-UTP and subsequently coupled to Cy3 and Cy5 dyes.
7	GSM12191	slide 1157	20,800	SA Pathology	2003-10-23	[Antibody] angiogenesis (GPL555)	RNA	Homo sapiens	vein	HUVEC from 3 different umbilical cords were plated onto 100ul of bovine type I collagen in 96-well microtitre plates (15 wells/cord). At times 0, 0.5, 3, 6 and 24 hours after stimulation with PMA and RMAC11, total RNA was isolated using the TRIZOL procedure. Message Amp aRNA Kit was used to linearly amplify the mRNA approximately 400-fold. The aRNA was generated incorporating 5-(3-aminoallyl)-UTP and subsequently coupled to Cy3 and Cy5 dyes.
8	GSM12192	slide 1158	20,800	SA Pathology	2003-10-23	[Antibody] angiogenesis (GPL555)	RNA	Homo sapiens	vein	HUVEC from 3 different umbilical cords were plated onto 100ul of bovine type I collagen in 96-well microtitre plates (15 wells/cord). At times 0, 0.5, 3, 6 and 24 hours after stimulation with PMA and RMAC11, total RNA was isolated using the TRIZOL procedure. Message Amp aRNA Kit was used to linearly amplify the mRNA approximately 400-fold. The aRNA was generated incorporating 5-(3-aminoallyl)-UTP and subsequently coupled to Cy3 and Cy5 dyes.
9	GSM12193	slide 1159	20,800	SA Pathology	2003-10-23	[Antibody] angiogenesis (GPL555)	RNA	Homo sapiens	vein	HUVEC from 3 different umbilical cords were plated onto 100ul of bovine type I collagen in 96-well microtitre plates (15 wells/cord). At times 0, 0.5, 3, 6 and 24 hours after stimulation with PMA and RMAC11, total RNA was isolated using the TRIZOL procedure. Message Amp aRNA Kit was used to linearly amplify the mRNA approximately 400-fold. The aRNA was generated incorporating 5-(3-aminoallyl)-UTP and subsequently coupled to Cy3 and Cy5 dyes.
10	GSM12194	slide 1160	20,800	SA Pathology	2003-10-23	[Antibody] angiogenesis (GPL555)	RNA	Homo sapiens	vein	HUVEC from 3 different umbilical cords were plated onto 100ul of bovine type I collagen in 96-well microtitre plates (15 wells/cord). At times 0, 0.5, 3, 6 and 24 hours after stimulation with PMA and RMAC11, total RNA was isolated using the TRIZOL procedure. Message Amp aRNA Kit was used to linearly amplify the mRNA approximately 400-fold. The aRNA was generated incorporating 5-(3-aminoallyl)-UTP and subsequently coupled to Cy3 and Cy5 dyes.
11	GSM12428	slide 1153	20,800	SA Pathology	2003-10-24	[Antibody] angiogenesis (GPL555)	RNA	Homo sapiens	vein	HUVEC from 3 different umbilical cords were plated onto 100ul of bovine type I collagen in 96-well microtitre plates (15 wells/cord). At times 0, 0.5, 3, 6 and 24 hours after stimulation with PMA and RMAC11, total RNA was isolated using the TRIZOL procedure. Message Amp aRNA Kit was used to linearly amplify the mRNA approximately 400-fold. The aRNA was generated incorporating 5-(3-aminoallyl)-UTP and subsequently coupled to Cy3 and Cy5 dyes.
12	GSM12429	slide 1154	20,800	SA Pathology	2003-10-24	[Antibody] angiogenesis (GPL555)	RNA	Homo sapiens	vein	HUVEC from 3 different umbilical cords were plated onto 100ul of bovine type I collagen in 96-well microtitre plates (15 wells/cord). At times 0, 0.5, 3, 6 and 24 hours after stimulation with PMA and RMAC11, total RNA was isolated using the TRIZOL procedure. Message Amp aRNA Kit was used to linearly amplify the mRNA approximately 400-fold. The aRNA was generated incorporating 5-(3-aminoallyl)-UTP and subsequently coupled to Cy3 and Cy5 dyes.
13	GSM12430	slide 1155	20,800	SA Pathology	2003-10-24	[Antibody] angiogenesis (GPL555)	RNA	Homo sapiens	vein	HUVEC from 3 different umbilical cords were plated onto 100ul of bovine type I collagen in 96-well microtitre plates (15 wells/cord). At times 0, 0.5, 3, 6 and 24 hours after stimulation with PMA and RMAC11, total RNA was isolated using the TRIZOL procedure. Message Amp aRNA Kit was used to linearly amplify the mRNA approximately 400-fold. The aRNA was generated incorporating 5-(3-aminoallyl)-UTP and subsequently coupled to Cy3 and Cy5 dyes.
14	GSM12431	slide 1156	20,800	SA Pathology	2003-10-24	[Antibody] angiogenesis (GPL555)	RNA	Homo sapiens	vein	HUVEC from 3 different umbilical cords were plated onto 100ul of bovine type I collagen in 96-well microtitre plates (15 wells/cord). At times 0, 0.5, 3, 6 and 24 hours after stimulation with PMA and RMAC11, total RNA was isolated using the TRIZOL procedure. Message Amp aRNA Kit was used to linearly amplify the mRNA approximately 400-fold. The aRNA was generated incorporating 5-(3-aminoallyl)-UTP and subsequently coupled to Cy3 and Cy5 dyes.
15	GSM12432	slide 1149	20,800	SA Pathology	2003-10-24	[Antibody] angiogenesis (GPL555)	RNA	Homo sapiens	vein	HUVEC from 3 different umbilical cords were plated onto 100ul of bovine type I collagen in 96-well microtitre plates (15 wells/cord). At times 0, 0.5, 3, 6 and 24 hours after stimulation with PMA and RMAC11, total RNA was isolated using the TRIZOL procedure. Message Amp aRNA Kit was used to linearly amplify the mRNA approximately 400-fold. The aRNA was generated incorporating 5-(3-aminoallyl)-UTP and subsequently coupled to Cy3 and Cy5 dyes. Keywords = angiogenesis Keywords = HUVEC Keywords = in vitro Keywords = capillary tube
16	GSM12433	slide 1150	20,800	SA Pathology	2003-10-24	[Antibody] angiogenesis (GPL555)	RNA	Homo sapiens	vein	HUVEC from 3 different umbilical cords were plated onto 100ul of bovine type I collagen in 96-well microtitre plates (15 wells/cord). At times 0, 0.5, 3, 6 and 24 hours after stimulation with PMA and RMAC11, total RNA was isolated using the TRIZOL procedure. Message Amp aRNA Kit was used to linearly amplify the mRNA approximately 400-fold. The aRNA was generated incorporating 5-(3-aminoallyl)-UTP and subsequently coupled to Cy3 and Cy5 dyes. Keywords = angiogenesis Keywords = HUVEC Keywords = in vitro Keywords = capillary tube
17	GSM12434	slide 1151	20,800	SA Pathology	2003-10-24	[Antibody] angiogenesis (GPL555)	RNA	Homo sapiens	vein	HUVEC from 3 different umbilical cords were plated onto 100ul of bovine type I collagen in 96-well microtitre plates (15 wells/cord). At times 0, 0.5, 3, 6 and 24 hours after stimulation with PMA and RMAC11, total RNA was isolated using the TRIZOL procedure. Message Amp aRNA Kit was used to linearly amplify the mRNA approximately 400-fold. The aRNA was generated incorporating 5-(3-aminoallyl)-UTP and subsequently coupled to Cy3 and Cy5 dyes. The cDNAs used in this microarray were identified as part of collaborative project between the IMVS and Bionomics Limited. The project aims were to identify genes up-regulated during in vitro capillary tube formation as targets for angiogenesis-based therapeutics. Any requests for further information regarding the data generated from this collaboration should be directed to Bionomics Limited (www.bionomics.com.au) at the following address: Bionomics Limited 31 Dalgleish Street Thebarton, South Australia Australia, 5031 Phone: 618 8354 6104 Fax: 618 8354 6199 Email: busdev@bionomics.com.au Keywords = angiogenesis Keywords = HUVEC Keywords = in vitro Keywords = capillary tube
18	GSM12435	slide 1152	20,800	SA Pathology	2003-10-24	[Antibody] angiogenesis (GPL555)	RNA	Homo sapiens	vein	HUVEC from 3 different umbilical cords were plated onto 100ul of bovine type I collagen in 96-well microtitre plates (15 wells/cord). At times 0, 0.5, 3, 6 and 24 hours after stimulation with PMA and RMAC11, total RNA was isolated using the TRIZOL procedure. Message Amp aRNA Kit was used to linearly amplify the mRNA approximately 400-fold. The aRNA was generated incorporating 5-(3-aminoallyl)-UTP and subsequently coupled to Cy3 and Cy5 dyes. The cDNAs used in this microarray were identified as part of collaborative project between the IMVS and Bionomics Limited. The project aims were to identify genes up-regulated during in vitro capillary tube formation as targets for angiogenesis-based therapeutics. Any requests for further information regarding the data generated from this collaboration should be directed to Bionomics Limited (www.bionomics.com.au) at the following address: Bionomics Limited 31 Dalgleish Street Thebarton, South Australia Australia, 5031 Phone: 618 8354 6104 Fax: 618 8354 6199 Email: busdev@bionomics.com.au Keywords = angiogenesis Keywords = HUVEC Keywords = in vitro Keywords = capillary tube
19	GSM1685	110 Antigens, mix #1, 12-9-99	2,304	Stanford Microarray Database (SMD)	2002-05-23	[Antibody] AB-8 (GPL173)	protein	Homo sapiens	unclassified	source_name:Reference antigens Test antigens title:110 Antigens, mix #1, 12-9-99 description:Category: Protein array Subcategory: Antibody Scanning Software: ScanAlyze Software version: 2.44 Lot batch = 8cl110
20	GSM1686	110 Antigens, mix #2, 12-9-99	2,304	Stanford Microarray Database (SMD)	2002-05-23	[Antibody] AB-8 (GPL173)	protein	Homo sapiens	unclassified	source_name:Reference antigens Test antigens title:110 Antigens, mix #2, 12-9-99 description:Category: Protein array Subcategory: Antibody Scanning Software: ScanAlyze Software version: 2.44 Lot batch = 8cl111

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