Gene Expression Omnibus (GEO) Overview

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DataSet	:	Series(GSE) x Platform(GPL). A set of related gene expression data.
Sample	:	Biological materials.
platform	:	Methods or instruments used for the gene expression profilings.

Human
(6,346)

Phages
(0)

All
(11,587)

SAGE RsaI
(0)

SAGE Sau3A
(0)

MPSS
(0)

GeneChip
(5,305)

Protein Array
(0)

Antibody
(0)

RT-PCR
(0)

HT-Seq
(0)

Other
(0)

adult aerial structure
(49)

young aerial structure
(0)

root
(20)

meristem/growing tissue
(8)

flower/sexual organ
(0)

seed/fruit/grain
(0)

pooled
(160)

unclassified
(547)

all
(5,305)

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	Sample ID	Title	Number of Data	Institute	Submission date	Platform	Sample type	Species	Organ class	Reasoning of the classification Keywords used for the classification are shown with bold font.
1	GSM102682	NS1	54,675	University of Pittsburgh	2006-03-31	[GeneChip] [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array (GPL570)	RNA	Homo sapiens	skin	normal skin
2	GSM102683	NS2	54,675	University of Pittsburgh	2006-03-31	[GeneChip] [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array (GPL570)	RNA	Homo sapiens	skin	normal skin
3	GSM102684	BN1	54,675	University of Pittsburgh	2006-03-31	[GeneChip] [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array (GPL570)	RNA	Homo sapiens	unclassified	source_name:benign nevi title:BN1 description:Intradermal nevus
4	GSM102685	BN2	54,675	University of Pittsburgh	2006-03-31	[GeneChip] [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array (GPL570)	RNA	Homo sapiens	unclassified	source_name:benign nevi title:BN2 description:Intradermal nevus
5	GSM102686	AN1	54,675	University of Pittsburgh	2006-03-31	[GeneChip] [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array (GPL570)	RNA	Homo sapiens	unclassified	source_name:atypical nevi title:AN1 description:Compound nevus with mild to moderate atypia
6	GSM102687	AN2	54,675	University of Pittsburgh	2006-03-31	[GeneChip] [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array (GPL570)	RNA	Homo sapiens	unclassified	source_name:atypical nevi title:AN2 description:Compound nevus with moderate atypia
7	GSM102688	in situ 1	54,675	University of Pittsburgh	2006-03-31	[GeneChip] [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array (GPL570)	RNA	Homo sapiens	skin	melanoma in situ
8	GSM102689	in situ 2	54,675	University of Pittsburgh	2006-03-31	[GeneChip] [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array (GPL570)	RNA	Homo sapiens	skin	melanoma in situ
9	GSM102691	VGP1	54,675	University of Pittsburgh	2006-03-31	[GeneChip] [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array (GPL570)	RNA	Homo sapiens	skin	VGP melanoma
10	GSM102692	VGP2	54,675	University of Pittsburgh	2006-03-31	[GeneChip] [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array (GPL570)	RNA	Homo sapiens	skin	VGP melanoma
11	GSM102695	MGP1	54,675	University of Pittsburgh	2006-03-31	[GeneChip] [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array (GPL570)	RNA	Homo sapiens	skin	MGP melanoma
12	GSM102696	MGP2	54,675	University of Pittsburgh	2006-03-31	[GeneChip] [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array (GPL570)	RNA	Homo sapiens	skin	MGP melanoma
13	GSM102697	LN1	54,675	University of Pittsburgh	2006-03-31	[GeneChip] [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array (GPL570)	RNA	Homo sapiens	lymphnode	lymph node metastasis
14	GSM102698	LN2	54,675	University of Pittsburgh	2006-03-31	[GeneChip] [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array (GPL570)	RNA	Homo sapiens	lymphnode	lymph node metastasis
15	GSM102699	LN3	54,675	University of Pittsburgh	2006-03-31	[GeneChip] [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array (GPL570)	RNA	Homo sapiens	lymphnode	lymph node metastasis
16	GSM102700	cultured MGP melanoma cells	54,675	University of Pittsburgh	2006-03-31	[GeneChip] [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array (GPL570)	RNA	Homo sapiens	skin	metastatic melanoma culture
17	GSM102701	Mel	54,675	University of Pittsburgh	2006-03-31	[GeneChip] [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array (GPL570)	RNA	Homo sapiens	skin	short term cultures of epidermal melanocytes
18	GSM102702	Ker	54,675	University of Pittsburgh	2006-03-31	[GeneChip] [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array (GPL570)	RNA	Homo sapiens	skin	short term cultures of epidermal keratinocytes
19	GSM102703	B RA 2	54,675	UniversitÃƒÂ© catholique de Louvain	2006-03-31	[GeneChip] [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array (GPL570)	RNA	Homo sapiens	unclassified	source_name:Rheumatoid arthritis patient title:B RA 2 description:Labeling of RNA (cRNA synthesis) was performed according to a standard AffymetrixÃ‚Â® procedure (One-Cycle Target Labeling kit, Affymetrix UK Ltd, High Wycombe, United Kingdom); briefly total RNA was first reverse transcribed into single-stranded cDNA using a T7-Oligo(dT) Promoter Primer and Superscript II reverse transcriptase. Next, Rnase H was added together with E. Coli DNA polymerase I and E. Coli DNA ligase, followed by a short incubation with T4 DNA polymerase in order to achieve synthesis of the second-strand cDNA. The double-stranded cDNA was purified and served as a template for the overnight in vitro transcription reaction, carried out in the presence of T7 RNA polymerase and a biotinylated nucleotide analog/ribonucleotide mix. At the end of this procedure, the biotinylated complementary RNA (cRNA) was cleaned, and fragmented by a 35 minute incubation at 95Ã‚Â°c. Because a minimal amount of 1 ug total RNA is required for the synthesis of cRNA, not all the CD20 cell samples could be processed, accounting for the lower number of CD20 cell hybridizations.
20	GSM102704	B RA 3	54,675	UniversitÃƒÂ© catholique de Louvain	2006-03-31	[GeneChip] [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array (GPL570)	RNA	Homo sapiens	unclassified	source_name:Rheumatoid arthritis patient title:B RA 3 description:Labeling of RNA (cRNA synthesis) was performed according to a standard AffymetrixÃ‚Â® procedure (One-Cycle Target Labeling kit, Affymetrix UK Ltd, High Wycombe, United Kingdom); briefly total RNA was first reverse transcribed into single-stranded cDNA using a T7-Oligo(dT) Promoter Primer and Superscript II reverse transcriptase. Next, Rnase H was added together with E. Coli DNA polymerase I and E. Coli DNA ligase, followed by a short incubation with T4 DNA polymerase in order to achieve synthesis of the second-strand cDNA. The double-stranded cDNA was purified and served as a template for the overnight in vitro transcription reaction, carried out in the presence of T7 RNA polymerase and a biotinylated nucleotide analog/ribonucleotide mix. At the end of this procedure, the biotinylated complementary RNA (cRNA) was cleaned, and fragmented by a 35 minute incubation at 95Ã‚Â°c. Because a minimal amount of 1 ug total RNA is required for the synthesis of cRNA, not all the CD20 cell samples could be processed, accounting for the lower number of CD20 cell hybridizations.

1 | 2 | 3 | 4 | 5 » [266]