| 1 |
GSM517406 |
Larvae rep 1 |
385,770 |
DEEDI |
2010-03-03 |
[Oligo Array] Nimblegen Rhipicephalus microplus 385K microarray (GPL10125) |
RNA |
Rhipicephalus microplus
 |
pooled |
unattached larvae (L - unfed) processed to provide a control larvae group without host stimulus A total of six tick naïve cattle, three Brahman and three Holstein-Friesian female cattle were infested with 1.5 g (~30,000) N strain larvae [31] and were kept grazing at the University of Queensland’s Pinjarra Hills campus. On Day 2, approximately 20,000 larvae were placed into a 24 cm2 mesh bag and attached to the neck of each animal for approx. 5 hrs in order for the larvae to ‘sense’ host stimuli while also in the presence of other attached ticks. These ‘frustrated’ larvae from both the Brahman (B-FL) and Holstein-Friesian (H-FL) were subsequently frozen in liquid nitrogen for total RNA extraction. An additional 20,000 unattached larvae (L - unfed) were processed to provide a control larvae group without host stimulus. At 17 days (soon after the 2nd tick moult from nymph to adult stages) approximately 500 young adult female ticks were collected from the Brahman and Holstein-Friesian cattle and frozen for total RNA extraction. The sampling regime was repeated to provide biological replicates of each sample to improve the statistical significance of the gene expression data (1 and 2 denote biological replicates). The experimental samples were: unattached/unfed larvae: L1, L2. “Frustrated†larvae: B-FL1; B-FL2 and HFL1; HFL 2. Attached adult female ticks (~17 days): – B-AT1, B-AT2 and H-AT1, H-AT2. (B=Brahman; H=Holstein-Friesian; FL=frustrated larvae; AT=adult tick) |
| 2 |
GSM517407 |
Larvae rep 2 |
385,770 |
DEEDI |
2010-03-03 |
[Oligo Array] Nimblegen Rhipicephalus microplus 385K microarray (GPL10125) |
RNA |
Rhipicephalus microplus
|
pooled |
unattached larvae (L - unfed) processed to provide a control larvae group without host stimulus A total of six tick naïve cattle, three Brahman and three Holstein-Friesian female cattle were infested with 1.5 g (~30,000) N strain larvae [31] and were kept grazing at the University of Queensland’s Pinjarra Hills campus. On Day 2, approximately 20,000 larvae were placed into a 24 cm2 mesh bag and attached to the neck of each animal for approx. 5 hrs in order for the larvae to ‘sense’ host stimuli while also in the presence of other attached ticks. These ‘frustrated’ larvae from both the Brahman (B-FL) and Holstein-Friesian (H-FL) were subsequently frozen in liquid nitrogen for total RNA extraction. An additional 20,000 unattached larvae (L - unfed) were processed to provide a control larvae group without host stimulus. At 17 days (soon after the 2nd tick moult from nymph to adult stages) approximately 500 young adult female ticks were collected from the Brahman and Holstein-Friesian cattle and frozen for total RNA extraction. The sampling regime was repeated to provide biological replicates of each sample to improve the statistical significance of the gene expression data (1 and 2 denote biological replicates). The experimental samples were: unattached/unfed larvae: L1, L2. “Frustrated†larvae: B-FL1; B-FL2 and HFL1; HFL 2. Attached adult female ticks (~17 days): – B-AT1, B-AT2 and H-AT1, H-AT2. (B=Brahman; H=Holstein-Friesian; FL=frustrated larvae; AT=adult tick) |
| 3 |
GSM517408 |
Brahman frustrate larvae rep 1 |
385,770 |
DEEDI |
2010-03-03 |
[Oligo Array] Nimblegen Rhipicephalus microplus 385K microarray (GPL10125) |
RNA |
Rhipicephalus microplus
|
pooled |
FrustrateLarvae into a mesh bag and attached to the neck of each Brahman bovine to sense host stimuli in the presence of other attached ticks A total of six tick naïve cattle, three Brahman and three Holstein-Friesian female cattle were infested with 1.5 g (~30,000) N strain larvae [31] and were kept grazing at the University of Queensland’s Pinjarra Hills campus. On Day 2, approximately 20,000 larvae were placed into a 24 cm2 mesh bag and attached to the neck of each animal for approx. 5 hrs in order for the larvae to ‘sense’ host stimuli while also in the presence of other attached ticks. These ‘frustrated’ larvae from both the Brahman (B-FL) and Holstein-Friesian (H-FL) were subsequently frozen in liquid nitrogen for total RNA extraction. An additional 20,000 unattached larvae (L - unfed) were processed to provide a control larvae group without host stimulus. At 17 days (soon after the 2nd tick moult from nymph to adult stages) approximately 500 young adult female ticks were collected from the Brahman and Holstein-Friesian cattle and frozen for total RNA extraction. The sampling regime was repeated to provide biological replicates of each sample to improve the statistical significance of the gene expression data (1 and 2 denote biological replicates). The experimental samples were: unattached/unfed larvae: L1, L2. “Frustrated†larvae: B-FL1; B-FL2 and HFL1; HFL 2. Attached adult female ticks (~17 days): – B-AT1, B-AT2 and H-AT1, H-AT2. (B=Brahman; H=Holstein-Friesian; FL=frustrated larvae; AT=adult tick) |
| 4 |
GSM517409 |
Holstein-Friesian frustrate larvae rep 1 |
385,770 |
DEEDI |
2010-03-03 |
[Oligo Array] Nimblegen Rhipicephalus microplus 385K microarray (GPL10125) |
RNA |
Rhipicephalus microplus
|
pooled |
Frustrate Larvae into a mesh bag and attached to the neck of each Holstein-Friesian bovine to sense host stimuli in the presence of other attached ticks A total of six tick naïve cattle, three Brahman and three Holstein-Friesian female cattle were infested with 1.5 g (~30,000) N strain larvae [31] and were kept grazing at the University of Queensland’s Pinjarra Hills campus. On Day 2, approximately 20,000 larvae were placed into a 24 cm2 mesh bag and attached to the neck of each animal for approx. 5 hrs in order for the larvae to ‘sense’ host stimuli while also in the presence of other attached ticks. These ‘frustrated’ larvae from both the Brahman (B-FL) and Holstein-Friesian (H-FL) were subsequently frozen in liquid nitrogen for total RNA extraction. An additional 20,000 unattached larvae (L - unfed) were processed to provide a control larvae group without host stimulus. At 17 days (soon after the 2nd tick moult from nymph to adult stages) approximately 500 young adult female ticks were collected from the Brahman and Holstein-Friesian cattle and frozen for total RNA extraction. The sampling regime was repeated to provide biological replicates of each sample to improve the statistical significance of the gene expression data (1 and 2 denote biological replicates). The experimental samples were: unattached/unfed larvae: L1, L2. “Frustrated†larvae: B-FL1; B-FL2 and HFL1; HFL 2. Attached adult female ticks (~17 days): – B-AT1, B-AT2 and H-AT1, H-AT2. (B=Brahman; H=Holstein-Friesian; FL=frustrated larvae; AT=adult tick) |
| 5 |
GSM517410 |
Brahman frustrate larvae rep 2 |
385,770 |
DEEDI |
2010-03-03 |
[Oligo Array] Nimblegen Rhipicephalus microplus 385K microarray (GPL10125) |
RNA |
Rhipicephalus microplus
|
pooled |
FrustrateLarvae into a mesh bag and attached to the neck of each Brahman bovine to sense host stimuli in the presence of other attached ticks A total of six tick naïve cattle, three Brahman and three Holstein-Friesian female cattle were infested with 1.5 g (~30,000) N strain larvae [31] and were kept grazing at the University of Queensland’s Pinjarra Hills campus. On Day 2, approximately 20,000 larvae were placed into a 24 cm2 mesh bag and attached to the neck of each animal for approx. 5 hrs in order for the larvae to ‘sense’ host stimuli while also in the presence of other attached ticks. These ‘frustrated’ larvae from both the Brahman (B-FL) and Holstein-Friesian (H-FL) were subsequently frozen in liquid nitrogen for total RNA extraction. An additional 20,000 unattached larvae (L - unfed) were processed to provide a control larvae group without host stimulus. At 17 days (soon after the 2nd tick moult from nymph to adult stages) approximately 500 young adult female ticks were collected from the Brahman and Holstein-Friesian cattle and frozen for total RNA extraction. The sampling regime was repeated to provide biological replicates of each sample to improve the statistical significance of the gene expression data (1 and 2 denote biological replicates). The experimental samples were: unattached/unfed larvae: L1, L2. “Frustrated†larvae: B-FL1; B-FL2 and HFL1; HFL 2. Attached adult female ticks (~17 days): – B-AT1, B-AT2 and H-AT1, H-AT2. (B=Brahman; H=Holstein-Friesian; FL=frustrated larvae; AT=adult tick) |
| 6 |
GSM517411 |
Holstein-Friesian frustrate larvae rep 2 |
385,770 |
DEEDI |
2010-03-03 |
[Oligo Array] Nimblegen Rhipicephalus microplus 385K microarray (GPL10125) |
RNA |
Rhipicephalus microplus
|
pooled |
Frustrate Larvae into a mesh bag and attached to the neck of each Holstein-Friesian bovine to sense host stimuli in the presence of other attached ticks A total of six tick naïve cattle, three Brahman and three Holstein-Friesian female cattle were infested with 1.5 g (~30,000) N strain larvae [31] and were kept grazing at the University of Queensland’s Pinjarra Hills campus. On Day 2, approximately 20,000 larvae were placed into a 24 cm2 mesh bag and attached to the neck of each animal for approx. 5 hrs in order for the larvae to ‘sense’ host stimuli while also in the presence of other attached ticks. These ‘frustrated’ larvae from both the Brahman (B-FL) and Holstein-Friesian (H-FL) were subsequently frozen in liquid nitrogen for total RNA extraction. An additional 20,000 unattached larvae (L - unfed) were processed to provide a control larvae group without host stimulus. At 17 days (soon after the 2nd tick moult from nymph to adult stages) approximately 500 young adult female ticks were collected from the Brahman and Holstein-Friesian cattle and frozen for total RNA extraction. The sampling regime was repeated to provide biological replicates of each sample to improve the statistical significance of the gene expression data (1 and 2 denote biological replicates). The experimental samples were: unattached/unfed larvae: L1, L2. “Frustrated†larvae: B-FL1; B-FL2 and HFL1; HFL 2. Attached adult female ticks (~17 days): – B-AT1, B-AT2 and H-AT1, H-AT2. (B=Brahman; H=Holstein-Friesian; FL=frustrated larvae; AT=adult tick) |
| 7 |
GSM517412 |
Brahman adult tick rep 1 |
385,770 |
DEEDI |
2010-03-03 |
[Oligo Array] Nimblegen Rhipicephalus microplus 385K microarray (GPL10125) |
RNA |
Rhipicephalus microplus
|
pooled |
17 days old (soon after the 2nd tick moult from nymph to adult stages) approximately 500 young adult female ticks were collected from the Brahman cattle A total of six tick naïve cattle, three Brahman and three Holstein-Friesian female cattle were infested with 1.5 g (~30,000) N strain larvae [31] and were kept grazing at the University of Queensland’s Pinjarra Hills campus. On Day 2, approximately 20,000 larvae were placed into a 24 cm2 mesh bag and attached to the neck of each animal for approx. 5 hrs in order for the larvae to ‘sense’ host stimuli while also in the presence of other attached ticks. These ‘frustrated’ larvae from both the Brahman (B-FL) and Holstein-Friesian (H-FL) were subsequently frozen in liquid nitrogen for total RNA extraction. An additional 20,000 unattached larvae (L - unfed) were processed to provide a control larvae group without host stimulus. At 17 days (soon after the 2nd tick moult from nymph to adult stages) approximately 500 young adult female ticks were collected from the Brahman and Holstein-Friesian cattle and frozen for total RNA extraction. The sampling regime was repeated to provide biological replicates of each sample to improve the statistical significance of the gene expression data (1 and 2 denote biological replicates). The experimental samples were: unattached/unfed larvae: L1, L2. “Frustrated†larvae: B-FL1; B-FL2 and HFL1; HFL 2. Attached adult female ticks (~17 days): – B-AT1, B-AT2 and H-AT1, H-AT2. (B=Brahman; H=Holstein-Friesian; FL=frustrated larvae; AT=adult tick) |
| 8 |
GSM517413 |
Holstein-Friesian adult tick rep 1 |
385,770 |
DEEDI |
2010-03-03 |
[Oligo Array] Nimblegen Rhipicephalus microplus 385K microarray (GPL10125) |
RNA |
Rhipicephalus microplus
|
pooled |
17 days old (soon after the 2nd tick moult from nymph to adult stages) approximately 500 young adult female ticks were collected from the Holstein-Friesian cattle A total of six tick naïve cattle, three Brahman and three Holstein-Friesian female cattle were infested with 1.5 g (~30,000) N strain larvae [31] and were kept grazing at the University of Queensland’s Pinjarra Hills campus. On Day 2, approximately 20,000 larvae were placed into a 24 cm2 mesh bag and attached to the neck of each animal for approx. 5 hrs in order for the larvae to ‘sense’ host stimuli while also in the presence of other attached ticks. These ‘frustrated’ larvae from both the Brahman (B-FL) and Holstein-Friesian (H-FL) were subsequently frozen in liquid nitrogen for total RNA extraction. An additional 20,000 unattached larvae (L - unfed) were processed to provide a control larvae group without host stimulus. At 17 days (soon after the 2nd tick moult from nymph to adult stages) approximately 500 young adult female ticks were collected from the Brahman and Holstein-Friesian cattle and frozen for total RNA extraction. The sampling regime was repeated to provide biological replicates of each sample to improve the statistical significance of the gene expression data (1 and 2 denote biological replicates). The experimental samples were: unattached/unfed larvae: L1, L2. “Frustrated†larvae: B-FL1; B-FL2 and HFL1; HFL 2. Attached adult female ticks (~17 days): – B-AT1, B-AT2 and H-AT1, H-AT2. (B=Brahman; H=Holstein-Friesian; FL=frustrated larvae; AT=adult tick) |
| 9 |
GSM517414 |
Brahman adult tick rep 2 |
385,770 |
DEEDI |
2010-03-03 |
[Oligo Array] Nimblegen Rhipicephalus microplus 385K microarray (GPL10125) |
RNA |
Rhipicephalus microplus
|
pooled |
17 days old (soon after the 2nd tick moult from nymph to adult stages) approximately 500 young adult female ticks were collected from the Brahman cattle A total of six tick naïve cattle, three Brahman and three Holstein-Friesian female cattle were infested with 1.5 g (~30,000) N strain larvae [31] and were kept grazing at the University of Queensland’s Pinjarra Hills campus. On Day 2, approximately 20,000 larvae were placed into a 24 cm2 mesh bag and attached to the neck of each animal for approx. 5 hrs in order for the larvae to ‘sense’ host stimuli while also in the presence of other attached ticks. These ‘frustrated’ larvae from both the Brahman (B-FL) and Holstein-Friesian (H-FL) were subsequently frozen in liquid nitrogen for total RNA extraction. An additional 20,000 unattached larvae (L - unfed) were processed to provide a control larvae group without host stimulus. At 17 days (soon after the 2nd tick moult from nymph to adult stages) approximately 500 young adult female ticks were collected from the Brahman and Holstein-Friesian cattle and frozen for total RNA extraction. The sampling regime was repeated to provide biological replicates of each sample to improve the statistical significance of the gene expression data (1 and 2 denote biological replicates). The experimental samples were: unattached/unfed larvae: L1, L2. “Frustrated†larvae: B-FL1; B-FL2 and HFL1; HFL 2. Attached adult female ticks (~17 days): – B-AT1, B-AT2 and H-AT1, H-AT2. (B=Brahman; H=Holstein-Friesian; FL=frustrated larvae; AT=adult tick) |
| 10 |
GSM517415 |
Holstein-Friesian adult tick rep 2 |
385,770 |
DEEDI |
2010-03-03 |
[Oligo Array] Nimblegen Rhipicephalus microplus 385K microarray (GPL10125) |
RNA |
Rhipicephalus microplus
|
pooled |
17 days old (soon after the 2nd tick moult from nymph to adult stages) approximately 500 young adult female ticks were collected from the Holstein-Friesian cattle A total of six tick naïve cattle, three Brahman and three Holstein-Friesian female cattle were infested with 1.5 g (~30,000) N strain larvae [31] and were kept grazing at the University of Queensland’s Pinjarra Hills campus. On Day 2, approximately 20,000 larvae were placed into a 24 cm2 mesh bag and attached to the neck of each animal for approx. 5 hrs in order for the larvae to ‘sense’ host stimuli while also in the presence of other attached ticks. These ‘frustrated’ larvae from both the Brahman (B-FL) and Holstein-Friesian (H-FL) were subsequently frozen in liquid nitrogen for total RNA extraction. An additional 20,000 unattached larvae (L - unfed) were processed to provide a control larvae group without host stimulus. At 17 days (soon after the 2nd tick moult from nymph to adult stages) approximately 500 young adult female ticks were collected from the Brahman and Holstein-Friesian cattle and frozen for total RNA extraction. The sampling regime was repeated to provide biological replicates of each sample to improve the statistical significance of the gene expression data (1 and 2 denote biological replicates). The experimental samples were: unattached/unfed larvae: L1, L2. “Frustrated†larvae: B-FL1; B-FL2 and HFL1; HFL 2. Attached adult female ticks (~17 days): – B-AT1, B-AT2 and H-AT1, H-AT2. (B=Brahman; H=Holstein-Friesian; FL=frustrated larvae; AT=adult tick) |
| 11 |
GSM678423 |
Untreated adult female ticks 1 rep 1 |
385,770 |
The University of Queensland |
2011-02-22 |
[Oligo Array] Nimblegen Rhipicephalus microplus 385K microarray (GPL10125) |
RNA |
Rhipicephalus microplus
|
unclassified |
source_name:Rhipicephalus (Boophilus) microplus (N strain) title:Untreated adult female ticks 1 rep 1 description:untreated control adult female ticks |
| 12 |
GSM678424 |
Untreated adult female ticks 1 rep 2 |
385,770 |
The University of Queensland |
2011-02-22 |
[Oligo Array] Nimblegen Rhipicephalus microplus 385K microarray (GPL10125) |
RNA |
Rhipicephalus microplus
|
unclassified |
source_name:Rhipicephalus (Boophilus) microplus (N strain) title:Untreated adult female ticks 1 rep 2 description:untreated control adult female ticks |
| 13 |
GSM678425 |
Untreated adult female ticks 2 rep 1 |
385,770 |
The University of Queensland |
2011-02-22 |
[Oligo Array] Nimblegen Rhipicephalus microplus 385K microarray (GPL10125) |
RNA |
Rhipicephalus microplus
|
unclassified |
source_name:Rhipicephalus (Boophilus) microplus (N strain) title:Untreated adult female ticks 2 rep 1 description:untreated control adult female ticks |
| 14 |
GSM678426 |
Untreated adult female ticks 2 rep 2 |
385,770 |
The University of Queensland |
2011-02-22 |
[Oligo Array] Nimblegen Rhipicephalus microplus 385K microarray (GPL10125) |
RNA |
Rhipicephalus microplus
|
unclassified |
source_name:Rhipicephalus (Boophilus) microplus (N strain) title:Untreated adult female ticks 2 rep 2 description:untreated control adult female ticks |
| 15 |
GSM678427 |
600bp TC6372 dsRNA injected adult female ticks 1 rep 1 |
385,770 |
The University of Queensland |
2011-02-22 |
[Oligo Array] Nimblegen Rhipicephalus microplus 385K microarray (GPL10125) |
RNA |
Rhipicephalus microplus
|
unclassified |
source_name:Rhipicephalus (Boophilus) microplus (N strain) title:600bp TC6372 dsRNA injected adult female ticks 1 rep 1 description:dsRNA injected adult female ticks |
| 16 |
GSM678428 |
600bp TC6372 dsRNA injected adult female ticks 1 rep 2 |
385,770 |
The University of Queensland |
2011-02-22 |
[Oligo Array] Nimblegen Rhipicephalus microplus 385K microarray (GPL10125) |
RNA |
Rhipicephalus microplus
|
unclassified |
source_name:Rhipicephalus (Boophilus) microplus (N strain) title:600bp TC6372 dsRNA injected adult female ticks 1 rep 2 description:dsRNA injected adult female ticks |
| 17 |
GSM678429 |
600bp TC6372 dsRNA injected adult female ticks 2 rep 1 |
385,770 |
The University of Queensland |
2011-02-22 |
[Oligo Array] Nimblegen Rhipicephalus microplus 385K microarray (GPL10125) |
RNA |
Rhipicephalus microplus
|
unclassified |
source_name:Rhipicephalus (Boophilus) microplus (N strain) title:600bp TC6372 dsRNA injected adult female ticks 2 rep 1 description:dsRNA injected adult female ticks |
| 18 |
GSM678430 |
600bp TC6372 dsRNA injected adult female ticks 2 rep 2 |
385,770 |
The University of Queensland |
2011-02-22 |
[Oligo Array] Nimblegen Rhipicephalus microplus 385K microarray (GPL10125) |
RNA |
Rhipicephalus microplus
|
unclassified |
source_name:Rhipicephalus (Boophilus) microplus (N strain) title:600bp TC6372 dsRNA injected adult female ticks 2 rep 2 description:dsRNA injected adult female ticks |
| 19 |
GSM215715 |
A02 |
317,503 |
QIMR |
2007-08-06 |
[Oligo Array] Sentrix BeadChip Array HumanHap300 Genotyping BeadChip 317K, TagSNP Phase I, v1.1 (GPL5711) |
genomic |
Homo sapiens
 |
skin |
Melanoma Cell Line |
| 20 |
GSM215716 |
A04 |
317,503 |
QIMR |
2007-08-06 |
[Oligo Array] Sentrix BeadChip Array HumanHap300 Genotyping BeadChip 317K, TagSNP Phase I, v1.1 (GPL5711) |
genomic |
Homo sapiens
|
skin |
Melanoma Cell Line |