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| 1 |
GPL2845 |
Applied Biosystems TaqMan Gene Expression Assays (human) |
210,442 |
Life Technologies |
2005-09-13 |
Applied Biosystems |
Homo sapiens
 |
RT-PCR |
RT-PCR, Applied Biosystems TaqMan Gene Expression Assays (human), Pre-designed TaqMan probe and primer sets for quantitative gene expression studies of human genes. |
| 2 |
GPL2850 |
Applied Biosystems TaqMan Gene Expression Assays (mouse) |
175,755 |
Life Technologies |
2005-09-15 |
Applied Biosystems |
Mus musculus
 |
RT-PCR |
RT-PCR, Applied Biosystems TaqMan Gene Expression Assays (mouse), Pre-designed TaqMan probe and primer sets for quantitative gene expression studies of mouse genes. |
| 3 |
GPL2847 |
Applied Biosystems TaqMan Gene Expression Assays (rat) |
150,478 |
Life Technologies |
2005-09-14 |
Applied Biosystems |
Rattus norvegicus
 |
RT-PCR |
RT-PCR, Applied Biosystems TaqMan Gene Expression Assays (rat), Pre-designed TaqMan probe and primer sets for quantitative gene expression studies of rat genes. |
| 4 |
GPL1624 |
PorkChip 2,600 cDNA array |
12,288 |
University of Minneosta |
2004-10-29 |
|
Sus scrofa
 |
RT-PCR |
spotted DNA/cDNA, PorkChip 2,600 cDNA array, Spotted cDNA array on glass. This set includes approximately 2,600 cDNA clones, selected from a Peyer’s patch subtracted library and other sources. The PorkChip2 cDNA microarray contains 2,423 ESTs from the subtracted jejunal Peyer’s patch library (UMNMPM3), 10 ESTs from the normal jejunal Peyer’s patch library (UMNMPM1), and 26 ESTs from the immune-stimulated jejunal Peyer’s patch library (UMNMPM2), which were cloned using the GeneTrapper cDNA Positive Selection System and pCMVSport6 vector (Invitrogen Corporation, Carlsbad, CA) (2). Each array also contains 86 ESTs identified by differential display RT-PCR (DD-RT-PCR) of PRRSV-infected macrophages (Library: PRRSV DD-RT-PCR)(5), 19 ESTs identified by DD-RT-PCR of influenza-infected porcine epithelial PK-15 cells (Library: UMNMPM4), 40 porcine genes, which were the result of directed cloning efforts (Library: Sus scrofa gene), and 7 porcine reproductive and respiratory syndrome virus strain VR-2332 sequences (Library: PRRSV) positive and negative controls (Library: Control). Positive controls included 8 Ambion ArrayControl sequences. The plasmid inserts were amplified by two rounds of nested PCR using vector-specific primers. The products from the second round PCR were cleaned by the Montage PCR(96) kit (Millipore, Billerica, MA) and were run on 0.8% agarose gels to quantify the amount of DNA by comparison to standards run on each gel. The dried PCR products were resuspended in phosphate buffer (150 mM sodium phosphate and 1 mM EDTA (pH 8.5)) at a final concentration of 100-300 ng/microliter and transferred to 384 well plates. A BioRobotics MicroGrid II (BioRobotics Ltd., Haslingfield, Cambridge, UK) arrayed the DNA on slides coated with poly-L-lysine (Sigma, St. Louis, MO)(3). Elements were arranged in 48 subarrays of 16 X 16, for a total of 12,288 spots per microarray, with each clone represented in by 4 spots. The slides were baked for 15 min at 80 C, UV irradiated (65 mJ), and chemically blocked with 1-methyl-2-pyrrolidinone and succinic anhydride (4) and were stored at room temperature under vacuum until use. The quality of each printing run was verified by staining one slide with SYBR Gold (Molecular Probes, Eugene, OR) and scanning at 488/530 nm (Excitation/ Emission) (1). Platform_references: 1. Battaglia C, Salani G, Consolandi C, Bernardi LR, and De Bellis G. Analysis of DNA microarrays by non-destructive fluorescent staining using SYBR green II. Biotechniques 29: 78-81, 2000. 2. Dvorak CMT, Hyland KA, Machado JG, Zhang Y, Fahrenkrug SC, and Murtaugh MP. Gene discovery and expression profiling in porcine Peyer's patch. Vet Immunol Immunopathol, submitted, 2004. 3. Eisen MB and Brown O. DNA arrays for analysis of gene expression. Methods Enzymol 303: 179-205, 1999. 4. Shalon D, Smith SJ, and Brown PO. A DNA microarray system for analyzing complex DNA samples using two-color fluorescent probe hybridization. Genome Res 6: 639-645, 1996. 5. Zhang X, Shin J, Molitor TW, Schook LB, and Rutherford MS. Molecular responses of macrophages to porcine reproductive and respiratory syndrome virus infection. Virology 262: 152-162, 1999. Keywords = small intestine, cDNA, pig |
| 5 |
GPL10963 |
PFGRC Staphylococcus_aureus_full_genome_RT-PCR |
2,647 |
J Craig Venter Institute |
2010-09-23 |
|
Staphylococcus aureus
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RT-PCR |
RT-PCR, PFGRC Staphylococcus_aureus_full_genome_RT-PCR, Full genome Staphylococcus aureus qRT-PCR |
| 6 |
GPL14610 |
Wafergen Biosystems SmartChip Human Oncology Panel v2 |
1,239 |
Cornell University |
2011-09-22 |
Wafergen Biosystems |
Homo sapiens
 |
RT-PCR |
RT-PCR, Wafergen Biosystems SmartChip Human Oncology Panel v2, SYBR Green qPCR assay |
| 7 |
GPL10974 |
Francisella tularensis full genome qRT-PCR |
1,068 |
J Craig Venter Institute |
2010-09-24 |
|
Francisella tularensis
 |
RT-PCR |
RT-PCR, Francisella tularensis full genome qRT-PCR, Francisella tularensis full genome qRT-PCR |
| 8 |
GPL4097 |
TaqMan Human MAQC (TAQ) |
1,044 |
NCTR/FDA |
2006-08-15 |
|
Homo sapiens
 |
RT-PCR |
RT-PCR, TaqMan Human MAQC (TAQ), |
| 9 |
GPL15373 |
Applied Biological Materials human miRNA (AMB C201) |
1,034 |
The University of British Columbia |
2012-03-22 |
Applied Biological Materials |
Homo sapiens
 |
RT-PCR |
RT-PCR, Applied Biological Materials human miRNA (AMB C201), PCR-based miRNA profiling assay |
| 10 |
GPL11665 |
Mouse Immunology 384 StellARrayâ„¢ qPCR Array plates (# 00188202) |
778 |
Imperial College London |
2011-01-27 |
Lonza/Bar Harbor Biotechnology |
Mus musculus
 |
RT-PCR |
RT-PCR, Mouse Immunology 384 StellARrayâ„¢ qPCR Array plates (# 00188202), This GEO Platform record represents two different plates, one custom made by Bar Harbor labs and another commercially available. This is designated by the 'plate' column in the Platform table below. |
| 11 |
GPL11199 |
Applied Biosystems Mouse Taqman miRNA Expression Assays A and B |
768 |
Mayo Clinic |
2010-11-11 |
Applied Biosystems |
Mus musculus
 |
RT-PCR |
RT-PCR, Applied Biosystems Mouse Taqman miRNA Expression Assays A and B, Cards A and B. |
| 12 |
GPL11316 |
Applied Biosystems Human Taqman MicroRNA Array v3.0 |
768 |
Johns Hopkins University School of Medicine |
2010-12-14 |
Applied Biosystems by Life Technologies |
Homo sapiens
 |
RT-PCR |
RT-PCR, Applied Biosystems Human Taqman MicroRNA Array v3.0, qRT-PCR TaqMan Low-Density Array |
| 13 |
GPL14907 |
SA Biosciences Human Genome V2.0 miRNA PCR Arrays: MAH-3200 (MAH-3100 & MAH-3101) |
768 |
University of Pittsburgh |
2011-11-21 |
SA Biosciences - A Qiagen Company |
Homo sapiens
 |
RT-PCR |
RT-PCR, SA Biosciences Human Genome V2.0 miRNA PCR Arrays: MAH-3200 (MAH-3100 & MAH-3101), |
| 14 |
GPL15466 |
TaqMan® Rodent MicroRNA Array v3.0 A/B |
768 |
Renji Hospital, JiaoTong University School of Medicine |
2012-04-19 |
Applied Biosystems |
Mus musculus
 |
RT-PCR |
RT-PCR, TaqMan® Rodent MicroRNA Array v3.0 A/B, PCR assay |
| 15 |
GPL15467 |
TaqMan® Human MicroRNA Array v3.0 A/B |
768 |
Renji Hospital, JiaoTong University School of Medicine |
2012-04-19 |
Applied Biosystems |
Homo sapiens
 |
RT-PCR |
RT-PCR, TaqMan® Human MicroRNA Array v3.0 A/B, PCR assay |
| 16 |
GPL15695 |
Exiqon microRNA Ready-to-Use PCR, Human panel I+II, V2.M |
768 |
Cancer Hospital, Fudan University |
2012-06-14 |
Exiqon |
Homo sapiens
 |
RT-PCR |
RT-PCR, Exiqon microRNA Ready-to-Use PCR, Human panel I+II, V2.M, miRCURY LNAâ„¢ Universal RT microRNA PCR, 4x Human panel I and 4x Human panel II in 384well PCR plates. One reaction each of 742 human microRNAs and 6 reference genes. For use with ABI 7900HT and other instruments. |
| 17 |
GPL16618 |
SABiosciences Human miRNome miRNA PCR Array [MAH-3200G] |
768 |
Instituto Mexicano del Seguro Social |
2013-02-12 |
SABiosciences |
Homo sapiens
 |
RT-PCR |
RT-PCR, SABiosciences Human miRNome miRNA PCR Array [MAH-3200G], PCR assay RT2 miRNA PCR Array: Human Genome V2.0 Complete (384-well)_Plates 1 & 2 Catalog Number: MAH-3200G SABiosciences Human Pediatric Astrocytomes IMSS |
| 18 |
GPL16850 |
Applied Biosystems TaqMan Array Human MicroRNA A+B Cards Set v3.0 |
768 |
The Chinese University of Hong Kong |
2013-03-20 |
Applied Biosystems, Life Technologies |
Homo sapiens
 |
RT-PCR |
RT-PCR, Applied Biosystems TaqMan Array Human MicroRNA A+B Cards Set v3.0, PCR assay |
| 19 |
GPL15497 |
Applied Biosystems TaqMan Array Human MicroRNA Cards (A+B Card Set v3) |
762 |
Universitätsklinikum Jena |
2012-04-27 |
Applied Biosystems |
Homo sapiens
 |
RT-PCR |
RT-PCR, Applied Biosystems TaqMan Array Human MicroRNA Cards (A+B Card Set v3), The TaqMan® Array Human MicroRNA Card Set v3.0 is a two card set containing a total of 384 TaqMan® MicroRNA Assays per card |
| 20 |
GPL4473 |
GUELPH Bovine immune-endocrine microarray |
720 |
University of Guelph |
2006-10-22 |
Immunogenetics lab, Department of Pathobiology, Universitu of Guelph |
Bos taurus
 |
RT-PCR |
spotted DNA/cDNA, GUELPH Bovine immune-endocrine microarray, The in-house microarray consists of 167 RT-PCR derived DNA fragments representing 167 ORFs from bovine. The DNA fragments are printed 4 times on the surface of the microarray (four-replicate blocks). The 167 immune and endocrine genes grouped into the following categories: cytokine genes, chemoattractants or chemokines and their respective receptor genes, immune regulatory genes, T-cell associated genes, pattern recognition or innate host defense genes, immunglobin (Ig) or Ig-related genes, complement genes, antigen processing or presenting genes, and genes encoding endocrine hormones or their receptors. As positive controls, the array includes 5 “housekeeping†genes (GAPDH, HPRT, PRL19, β-actin, β2-microglobulin), negative controls consisted of a Rhodococcus equi 17kD virulence protein gene, plasmid pACYC177 DNA, and a thaliana photosystem I chlorophyll a/b-binding protein gene, and the FcRgII gene intron. The printing controls included serially diluted bovine genomic DNA, and printing buffer only. |
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