| 1 |
GPL1624 |
PorkChip 2,600 cDNA array |
12,288 |
University of Minneosta |
2004-10-29 |
|
Sus scrofa
 |
RT-PCR |
spotted DNA/cDNA, PorkChip 2,600 cDNA array, Spotted cDNA array on glass. This set includes approximately 2,600 cDNA clones, selected from a Peyer’s patch subtracted library and other sources. The PorkChip2 cDNA microarray contains 2,423 ESTs from the subtracted jejunal Peyer’s patch library (UMNMPM3), 10 ESTs from the normal jejunal Peyer’s patch library (UMNMPM1), and 26 ESTs from the immune-stimulated jejunal Peyer’s patch library (UMNMPM2), which were cloned using the GeneTrapper cDNA Positive Selection System and pCMVSport6 vector (Invitrogen Corporation, Carlsbad, CA) (2). Each array also contains 86 ESTs identified by differential display RT-PCR (DD-RT-PCR) of PRRSV-infected macrophages (Library: PRRSV DD-RT-PCR)(5), 19 ESTs identified by DD-RT-PCR of influenza-infected porcine epithelial PK-15 cells (Library: UMNMPM4), 40 porcine genes, which were the result of directed cloning efforts (Library: Sus scrofa gene), and 7 porcine reproductive and respiratory syndrome virus strain VR-2332 sequences (Library: PRRSV) positive and negative controls (Library: Control). Positive controls included 8 Ambion ArrayControl sequences. The plasmid inserts were amplified by two rounds of nested PCR using vector-specific primers. The products from the second round PCR were cleaned by the Montage PCR(96) kit (Millipore, Billerica, MA) and were run on 0.8% agarose gels to quantify the amount of DNA by comparison to standards run on each gel. The dried PCR products were resuspended in phosphate buffer (150 mM sodium phosphate and 1 mM EDTA (pH 8.5)) at a final concentration of 100-300 ng/microliter and transferred to 384 well plates. A BioRobotics MicroGrid II (BioRobotics Ltd., Haslingfield, Cambridge, UK) arrayed the DNA on slides coated with poly-L-lysine (Sigma, St. Louis, MO)(3). Elements were arranged in 48 subarrays of 16 X 16, for a total of 12,288 spots per microarray, with each clone represented in by 4 spots. The slides were baked for 15 min at 80 C, UV irradiated (65 mJ), and chemically blocked with 1-methyl-2-pyrrolidinone and succinic anhydride (4) and were stored at room temperature under vacuum until use. The quality of each printing run was verified by staining one slide with SYBR Gold (Molecular Probes, Eugene, OR) and scanning at 488/530 nm (Excitation/ Emission) (1). Platform_references: 1. Battaglia C, Salani G, Consolandi C, Bernardi LR, and De Bellis G. Analysis of DNA microarrays by non-destructive fluorescent staining using SYBR green II. Biotechniques 29: 78-81, 2000. 2. Dvorak CMT, Hyland KA, Machado JG, Zhang Y, Fahrenkrug SC, and Murtaugh MP. Gene discovery and expression profiling in porcine Peyer's patch. Vet Immunol Immunopathol, submitted, 2004. 3. Eisen MB and Brown O. DNA arrays for analysis of gene expression. Methods Enzymol 303: 179-205, 1999. 4. Shalon D, Smith SJ, and Brown PO. A DNA microarray system for analyzing complex DNA samples using two-color fluorescent probe hybridization. Genome Res 6: 639-645, 1996. 5. Zhang X, Shin J, Molitor TW, Schook LB, and Rutherford MS. Molecular responses of macrophages to porcine reproductive and respiratory syndrome virus infection. Virology 262: 152-162, 1999. Keywords = small intestine, cDNA, pig |
| 2 |
GPL10963 |
PFGRC Staphylococcus_aureus_full_genome_RT-PCR |
2,647 |
J Craig Venter Institute |
2010-09-23 |
|
Staphylococcus aureus
 |
RT-PCR |
RT-PCR, PFGRC Staphylococcus_aureus_full_genome_RT-PCR, Full genome Staphylococcus aureus qRT-PCR |
| 3 |
GPL11111 |
Salmonella enterica subsp. enterica serovar Typhimurium str. 14028S 0.3K |
288 |
J Craig Venter Institute |
2010-10-25 |
|
Salmonella enterica subsp. enterica serovar Typhimurium str. 14028S
 |
RT-PCR |
RT-PCR, Salmonella enterica subsp. enterica serovar Typhimurium str. 14028S 0.3K, |
| 4 |
GPL2847 |
Applied Biosystems TaqMan Gene Expression Assays (rat) |
150,478 |
Life Technologies |
2005-09-14 |
Applied Biosystems |
Rattus norvegicus
 |
RT-PCR |
RT-PCR, Applied Biosystems TaqMan Gene Expression Assays (rat), Pre-designed TaqMan probe and primer sets for quantitative gene expression studies of rat genes. |
| 5 |
GPL16145 |
Applied Biosystems Open Array Custom Assays (rat) |
110 |
Ontario Institute of Cancer Research |
2012-10-05 |
Applied Biosystems |
Rattus norvegicus
 |
RT-PCR |
RT-PCR, Applied Biosystems Open Array Custom Assays (rat), PCR assay |
| 6 |
GPL16146 |
Applied Biosystems qPCR Custom Assays (rat) |
105 |
Ontario Institute of Cancer Research |
2012-10-05 |
Applied Biosystems |
Rattus norvegicus
 |
RT-PCR |
RT-PCR, Applied Biosystems qPCR Custom Assays (rat), PCR assay |
| 7 |
GPL13888 |
Weiss SABiosciences Custom RT-PCR profiler array CAPR 100083, 96, v1.0 |
96 |
Kansas State University |
2011-07-12 |
SABiosciences - Qiagen |
Rattus norvegicus
 |
RT-PCR |
RT-PCR, Weiss SABiosciences Custom RT-PCR profiler array CAPR 100083, 96, v1.0, rat ESC RT-PCR custom array, see information on layout of array below, see PCR efficiency data provided by manufacturer, below. SABiosciences RT² Profiler panel of PCR assays. |
| 8 |
GPL14605 |
Applied Biosystems Low Density Taquman Rat Inflammatory Array |
96 |
University of South Carolina-School of Medicine |
2011-09-22 |
Applied Biosystems |
Rattus norvegicus
 |
RT-PCR |
RT-PCR, Applied Biosystems Low Density Taquman Rat Inflammatory Array, see manufacturer's website |
| 9 |
GPL1793 |
Rainbow trout sex differentiation cascade-related genes investigated using RT-PCR |
102 |
Institut National de la Recherche Agronomique-INRA |
2005-01-12 |
|
Oncorhynchus mykiss
 |
RT-PCR |
RT-PCR, Rainbow trout sex differentiation cascade-related genes investigated using RT-PCR, Virtual platform listing sex differentiation cascade-related candidate genes investigated using RT-PCR. |
| 10 |
GPL10331 |
TaqMan® Rodent miRNA A+B Cards Set v2.0 |
471 |
health canada |
2010-04-15 |
Applied Biosystem |
Mus musculus,Rattus norvegicus
 |
RT-PCR |
RT-PCR, TaqMan® Rodent miRNA A+B Cards Set v2.0, The TaqMan® Rodent MicroRNA Array Set v2.0 is a two card set containing a total of 364 TaqMan® MicroRNA Assays per card. The set enables accurate quantitation of 518 and 303 unique microRNAs for mouse and rat respectively in as little as 5 hours. Included on each array is three TaqMan® MicroRNA Assay endogenous controls for each species to aid in data normalization and one TaqMan® MicroRNA Assay not related to human as a negative control. Details on the included assays is available in the Target List file. Purchase of the Megaplex RT Primers, Rodent Pool Set is required to run this array set. An additional preamplification step is enabled by Megaplex PreAmp Primers, Rodent Pool Set for situations where sensitivity is of the utmost importance and/or sample is limiting. |
| 11 |
GPL11636 |
TaqMan® Rodent miRNA card A v2.0 |
384 |
Stanford University School of Medicine |
2011-01-20 |
|
Mus musculus,Rattus norvegicus
 |
RT-PCR |
RT-PCR, TaqMan® Rodent miRNA card A v2.0, A Card Content: MicroRNAs of demonstrated interest to the research community (these tend to be functionally defined, broadly expressed and/or highly expressed). |
| 12 |
GPL11637 |
TaqMan® Rodent miRNA Card B v2.0 |
384 |
Stanford University School of Medicine |
2011-01-20 |
|
Mus musculus,Rattus norvegicus
 |
RT-PCR |
RT-PCR, TaqMan® Rodent miRNA Card B v2.0, B Card Content: MicroRNAs less extensively studied (these tend to have less defined functions, be narrowly expressed, and/or be expressed at a low level). |
| 13 |
GPL14646 |
TaqMan Rodent MicroRNA A Array v2.0 |
384 |
Stanford University |
2011-10-02 |
Applied Biosystems |
Mus musculus,Rattus norvegicus
 |
RT-PCR |
RT-PCR, TaqMan Rodent MicroRNA A Array v2.0, TaqMan array rodent microRNA A Card v2.0 Real-time PCR based microRNA microarrays |
| 14 |
GPL15350 |
Applied Biosystems TaqMan Rodent MicroRNA Array A |
384 |
SBMRI |
2012-03-14 |
Applied Biosystems |
Mus musculus,Rattus norvegicus
 |
RT-PCR |
RT-PCR, Applied Biosystems TaqMan Rodent MicroRNA Array A, PCR assay Standard TaqMan Assay |
| 15 |
GPL15351 |
Applied Biosystems TaqMan Rodent MicroRNA Array B |
384 |
SBMRI |
2012-03-14 |
Applied Biosystems |
Mus musculus,Rattus norvegicus
 |
RT-PCR |
RT-PCR, Applied Biosystems TaqMan Rodent MicroRNA Array B, PCR assay Standard TaqMan Assay |
| 16 |
GPL16178 |
TaqMan Rodent microRNA A Card v3.0 |
381 |
Kennedy Institute of Rheumatology |
2012-10-15 |
Applied Biosystems |
Mus musculus,Rattus norvegicus
 |
RT-PCR |
RT-PCR, TaqMan Rodent microRNA A Card v3.0, |
| 17 |
GPL2850 |
Applied Biosystems TaqMan Gene Expression Assays (mouse) |
175,755 |
Life Technologies |
2005-09-15 |
Applied Biosystems |
Mus musculus
 |
RT-PCR |
RT-PCR, Applied Biosystems TaqMan Gene Expression Assays (mouse), Pre-designed TaqMan probe and primer sets for quantitative gene expression studies of mouse genes. |
| 18 |
GPL11665 |
Mouse Immunology 384 StellARrayâ„¢ qPCR Array plates (# 00188202) |
778 |
Imperial College London |
2011-01-27 |
Lonza/Bar Harbor Biotechnology |
Mus musculus
 |
RT-PCR |
RT-PCR, Mouse Immunology 384 StellARrayâ„¢ qPCR Array plates (# 00188202), This GEO Platform record represents two different plates, one custom made by Bar Harbor labs and another commercially available. This is designated by the 'plate' column in the Platform table below. |
| 19 |
GPL11199 |
Applied Biosystems Mouse Taqman miRNA Expression Assays A and B |
768 |
Mayo Clinic |
2010-11-11 |
Applied Biosystems |
Mus musculus
 |
RT-PCR |
RT-PCR, Applied Biosystems Mouse Taqman miRNA Expression Assays A and B, Cards A and B. |
| 20 |
GPL15466 |
TaqMan® Rodent MicroRNA Array v3.0 A/B |
768 |
Renji Hospital, JiaoTong University School of Medicine |
2012-04-19 |
Applied Biosystems |
Mus musculus
 |
RT-PCR |
RT-PCR, TaqMan® Rodent MicroRNA Array v3.0 A/B, PCR assay |