| 1 |
GPL317 |
SHCO |
43,008 |
Stanford University |
2003-05-07 |
|
Homo sapiens
 |
cDNA Array |
spotted DNA/cDNA, SHCO, Tip Configuration: Standard 48 tip Columns per Sector: 30 Rows per Sector: 30 Column Spacing: 146 Row Spacing: 146 |
| 2 |
GPL318 |
SHCZ |
43,008 |
Stanford University |
2003-05-07 |
|
Homo sapiens
|
cDNA Array |
spotted DNA/cDNA, SHCZ, Tip Configuration: Standard 48 tip Columns per Sector: 30 Rows per Sector: 30 Column Spacing: 146 Row Spacing: 146 |
| 3 |
GPL319 |
SHDJ |
43,008 |
Stanford University |
2003-05-07 |
|
Homo sapiens
|
cDNA Array |
spotted DNA/cDNA, SHDJ, Tip Configuration: Standard 48 tip Columns per Sector: 30 Rows per Sector: 30 Column Spacing: 146 Row Spacing: 146 |
| 4 |
GPL284 |
Human UniGene Set RZPD 1 |
34,560 |
Christian-Albrechts-University Kiel |
2003-04-10 |
|
Homo sapiens
|
cDNA Array |
spotted DNA/cDNA, Human UniGene Set RZPD 1, Preparation of high density cDNA arrays. cDNA clone inserts (not sequence-verified) from eighty-eight 384-well microtitre plates from the Human Unigene 1 clone set (RZPD Deutsches Ressourcenzentrum für Genomforschung and Boer et al. 2001) and a custom-made plate of an additional 258 clones (sequence-verified) were amplified by PCR in a 384-well format using M13 forward (5`-CGTTGTAAAACGACGGCCAGT-3´) and reverse (5´-TTTCACACAGGAAACAGCTATGAC-3´) primers. The PCR reaction was set up by dipping a 384-pin replicator (Genetix, UK) twice into the bacterial library plate and transferring the solution (approximately 0.2 µL) to a new PCR plate containing 25µL of PCR master mix in each well (final concentrations: 1 X PCR buffer (0.5M Tris-HCl (pH 8.6), 0.5M KCl, 15mM MgCl2, 1% Tween 20), betaine (1.5U), 0.2mM dNTPs, 0.2mM primers and 0.1U/µL Taq/Pfu). Amplification of PCR products was carried out as follows: 95ºC for 1 min, 94ºC for 15 sec, 59ºC for 15 sec, 65ºC for 55 sec, repeat of 59ºC for 15 sec, 65ºC for 55 sec for an additional 2 cycles, repeat of 94ºC for 15 sec, 59ºC for 15 sec, 65ºC for 55 sec for 29 cycles and finally 68ºC for 10 min. The sub-cycling step described improved product yield and stability. The quality of the PCR reaction was determined visually by slot-blot electrophoresis on 1% agarose gels. Prior to spotting, PCR products were denatured by the addition of a final concentration of 0.1M NaOH to each well. The ~34,000 PCR products were repeatedly spotted 7 times in a 6 X 6 pattern (asymmetrically to ensure ease in determining filter orientation) on dry 23 X 23 cm Hybond N+ nylon membranes (Amersham Pharmacia) using a Genetix robot attached to a 384 pin gadget-head (250 mm pins). The 6 X 6 spotting blocks were organized into six fields of 384 spotting blocks (arranged 16 X 24), resulting in 2304 spotting blocks on each filter. Each 6 X 6 spotting block contained duplicate PCR products from 15 clones (14 clones in field 6), duplicate Arabidoposis thaliana clones (GenBank accession numbers AF104328 and U29785, derived from the Arabidopsis Biological Resource Center and DNA stock donor at Ohio State University) serve as guide spots during subsequent gridding steps, and four (six in field 6) empty positions to allow for an estimation of background intensity after hybridization. In order to determine the quality of the spotting, 1.58 µM fluorescin was added to the Arabidoposis thaliana control clone plate and the filter was scanned using an ‘in-house’ work station for high resolution fluorescence detection (MPI for Molecular Genetics, Berlin). In this way, unevenly or poorly spotted filters were discarded prior to hybridization. After spotting, cDNA on the filters was further denatured by floating filters (face up) in 0.4M NaOH for 2 min and neutralized with 5 X SSC (pH 7.5) for 2 min. The DNA was cross-linked to the filter by heat-baking at 80°C for 30 min and exposing to ultraviolet radiation (Stratalinker UV cross-linker (auto cross-link option); Stratagene). Filters were stored dry at 4°C until ready for use. Reference: Boer, J.M., Huber, W.K., Sultmann, H., Wilmer, F., von Heydebreck, A., Haas, S., Korn, B., Gunawan, B., Vente, A., Fuzesi, L. et al. (2001) Identification and classification of differentially expressed genes in renal cell carcinoma by expression profiling on a global human 31,500- element cDNA array. Genome Res, 11, 1861-1870. |
| 5 |
GPL321 |
M20K (Incyte mouse release 1.0 and Unigene1) |
21,168 |
St. Jude Children's Research Hospital |
2003-05-14 |
|
Mus musculus
 |
cDNA Array |
spotted DNA/cDNA, M20K (Incyte mouse release 1.0 and Unigene1), The M20K microarray includes 18,330 cDNA mouse clones from the Incyte mouse release 1.0 and Unigene 1 collections. The PCR amplified cDNA inserts were either obtained from Incyte or were amplified from replica plate cultures, purified and mechanically spotted onto polylysine-coated microscope slides using an OmniGrid robotic spotter (GeneMachines, San Carlo, CA). Printed slides were post-processed according to the procedures on the Stanford website with some modifications: http://cmgm.stanford.edu/pbrown/protocols/index.html. |
| 6 |
GPL325 |
NBFGC cDNA microarray |
19,200 |
Michigan State University |
2003-05-15 |
|
Bos taurus
 |
cDNA Array |
spotted DNA/cDNA, NBFGC cDNA microarray, National Bovine Functional Genomics Consortium cDNA microarray. Keywords = cDNA, microarray, bovine |
| 7 |
GPL309 |
NBFGC_1 |
19,194 |
Michigan State University |
2003-04-24 |
|
Bos taurus
|
cDNA Array |
spotted DNA/cDNA, NBFGC_1, National Bovine Functional Genomics Consortium microarray version 1 Keywords = bovine microarray EST cDNA |
| 8 |
GPL316 |
Human 18K set (8.13.2001) |
17,754 |
Fred Hutchinson Cancer Research Center |
2003-05-07 |
|
Homo sapiens
|
cDNA Array |
spotted DNA/cDNA, Human 18K set (8.13.2001), Human 18K set created on 08/13/2001 Keywords = Hela, transformation |
| 9 |
GPL324 |
M3 (NIA Mouse 15K Clone Set) |
17,329 |
St. Jude Children's Research Hospital |
2003-05-14 |
|
Mus musculus
|
cDNA Array |
spotted DNA/cDNA, M3 (NIA Mouse 15K Clone Set), The M3 microarray includes cDNAs from the NIA Mouse 15K cDNA clone collection. NIA Mouse 15K is derived from several early embryonic cDNA libraries, including blastocyst, preimplantation, embryonic stem cells, trophoblast stem cells, embryonic germ cells, and others. The cDNA inserts were amplified from replica plate cultures by PCR, purified and mechanically spotted onto polylysine-coated microscope slides using an OmniGrid robotic spotter (GeneMachines, San Carlo, CA). Printed slides were post-processed according to the procedures on the Stanford website with some modifications: http://cmgm.stanford.edu/pbrown/protocols/index.html. |
| 10 |
GPL323 |
Ret13K (BMAP mouse Retinal library) |
17,328 |
St. Jude Children's Research Hospital |
2003-05-14 |
|
Mus musculus
|
cDNA Array |
spotted DNA/cDNA, Ret13K (BMAP mouse Retinal library), The Ret13K microarray includes 12,243 mouse clones including BMAP mouse retinal clones and 220 clones from the Incyte mouse unigene 1 collection. The cDNA inserts were amplified from replica plate cultures by PCR, purified and mechanically spotted onto polylysine-coated microscope slides using an OmniGrid robotic spotter (GeneMachines, San Carlo, CA). Printed slides were post-processed according to the procedures on the Stanford website with some modifications: http://cmgm.stanford.edu/pbrown/protocols/index.html. |
| 11 |
GPL311 |
CDMC_Drosophila_7k2 |
16,896 |
University of Toronto |
2003-04-28 |
|
Drosophila melanogaster
 |
cDNA Array |
spotted DNA/cDNA, CDMC_Drosophila_7k2, The CDMC_Drosophila_7K2 array is a primarily cDNA-based Drosophila melanogaster glass microarray. The array features 5,756 Berkeley Drosophila Genome Project cDNAs, 1,078 NIH Testis cDNAs (Andrews et al. 2000) and 546 gene fragments that were amplified from genomic DNA, together representing approximately 50% of the predicted genes in D. melanogaster. The array is produced by the Canadian Drosophila Microarray Centre at the University of Toronto and is available for distribution to academic labs. Keywords = CDMC Keywords = Drosophila Keywords = microarray Keywords = cDNA |
| 12 |
GPL345 |
H10K(Incyte Human Unigene 1) |
11,856 |
St. Jude Children's Research Hospital |
2003-06-23 |
|
Homo sapiens
|
cDNA Array |
spotted DNA/cDNA, H10K(Incyte Human Unigene 1), The H10K microarray includes 9182 cDNA human clones from the Incyte Human Unigene 1 collection. The cDNA inserts were amplified from replica plate cultures by PCR, purified and mechanically spotted onto polylysine-coated microscope slides using an OmniGrid robotic spotter (GeneMachines, San Carlo, CA). Printed slides were post-processed according to the procedures on the Stanford website with some modifications: http://cmgm.stanford.edu/pbrown/protocols/index.html. |
| 13 |
GPL322 |
H1 (Incyte Human Unigene 1) |
11,552 |
St. Jude Children's Research Hospital |
2003-05-14 |
|
Homo sapiens
|
cDNA Array |
spotted DNA/cDNA, H1 (Incyte Human Unigene 1), The H1 microarray includes 9182 cDNA human clones from the Incyte Human Unigene 1 collection. The cDNA inserts were amplified from replica plate cultures by PCR, purified and mechanically spotted onto polylysine-coated microscope slides using an OmniGrid robotic spotter (GeneMachines, San Carlo, CA). Printed slides were post-processed according to the procedures on the Stanford website with some modifications: http://cmgm.stanford.edu/pbrown/protocols/index.html. |
| 14 |
GPL344 |
M4(Incyte mouse Unigene1) |
11,552 |
St. Jude Children's Research Hospital |
2003-06-23 |
|
Mus musculus
|
cDNA Array |
spotted DNA/cDNA, M4(Incyte mouse Unigene1), The M4 microarray includes 9,596 cDNA mouse clones from the Incyte mouse Unigene 1 collection. The PCR amplified cDNA inserts were either obtained from Incyte or were amplified from replica plate cultures, purified and mechanically spotted onto polylysine-coated microscope slides using an OmniGrid robotic spotter (GeneMachines, San Carlo, CA). Printed slides were post-processed according to the procedures on the Stanford website with some modifications: http://cmgm.stanford.edu/pbrown/protocols/index.html. |
| 15 |
GPL346 |
M2(Incyte mouse release 1.0) |
11,552 |
St. Jude Children's Research Hospital |
2003-06-23 |
|
Mus musculus
|
cDNA Array |
spotted DNA/cDNA, M2(Incyte mouse release 1.0), The M2 microarray includes 8,734 cDNA mouse clones from the Incyte mouse release 1.0 collection. The PCR amplified cDNA inserts were either obtained from Incyte or were amplified from replica plate cultures, purified and mechanically spotted onto polylysine-coated microscope slides using an OmniGrid robotic spotter (GeneMachines, San Carlo, CA). Printed slides were post-processed according to the procedures on the Stanford website with some modifications: http://cmgm.stanford.edu/pbrown/protocols/index.html. |
| 16 |
GPL334 |
Human 10K - Wellcome Trust Sanger Institute |
9,984 |
University of Oxford, John Radcliffe Hospital |
2003-06-06 |
|
Homo sapiens
|
cDNA Array |
spotted DNA/cDNA, Human 10K - Wellcome Trust Sanger Institute, 10K cDNA microarray manufactured at the Microarray Facility, The Wellcome Trust Sanger Institute, Cambridge, UK. |
| 17 |
GPL338 |
BLI_array_NIAS |
9,216 |
National Institute of Agrobiological Sciences |
2003-06-17 |
|
Bos taurus
|
cDNA Array |
spotted DNA/cDNA, BLI_array_NIAS, BLI_array_NIAS platform of a spotted cDNA microarray has been established using a bovine liver cDNA library. The array has been fabricated collaboratively by Laboratory of Reproductive Biology and Technology at National Institute of Agrobiological Sciences, Tsukuba Science City, Japan and Department of Paediatric Pharmacology at National Institute of Child Health and Development, Tokyo, Japan. Keywords = bovine liver, cDNA array, gene expression |
| 18 |
GPL335 |
Incyte Human UniGEM V .14 |
7,166 |
UT Southwestern Medical Center |
2003-06-09 |
|
Homo sapiens
|
cDNA Array |
spotted DNA/cDNA, Incyte Human UniGEM V .14, The UniGEM V gene expression microarray contains 7,075 sequence-verified human cDNA clones for both known genes and ESTs. UniGEM V clones include a broad spectrum of human genes whose responses can be measured for all types of experimental systems. The clones are derived from Incyte’s set of sequence-verified cDNA clones and represent unique human genes identified in NCBI’s UniGene database, build 46. For more information about UniGene, visit http://www.ncbi.nlm.nih.gov/UniGene/ Keywords = high density cDNA microarray |
| 19 |
GPL289 |
Segal_3 |
6,111 |
Stanford University |
2003-04-15 |
|
Saccharomyces cerevisiae
 |
cDNA Array |
spotted DNA/cDNA, Segal_3, Used to examine wild type and ypl230w mutant under stationary phase. |
| 20 |
GPL287 |
Segal_1 |
5,993 |
Stanford University |
2003-04-15 |
|
Saccharomyces cerevisiae
|
cDNA Array |
spotted DNA/cDNA, Segal_1, Used to examine wild type and kin82 mutant under heat shock. |
|