| 1 |
GSM10325 |
Reciprocity-2h high UV-B |
17,296 |
Universidad Nacional de Rosario |
2003-09-18 |
[cDNA Array] ZmDB Array Unigene-1-01-07 (GPL499) |
RNA |
Zea mays
 |
pooled |
Microarray hybridization was used to assess UV-B responses in both directly exposed and shielded tissues of a maize line deficient in anthocyanin accumulation. Among 347 transcripts found to be regulated after 8 h of high UV-B irradiation, 285 were increased significantly in at least one organ but only 80 were down-regulated by UV-B. Although some genes were regulated by UV-B in more than one sample, most UV-B regulated genes were organ-specific. A larger number of transcript changes occurred in directly exposed than in shielded organs, and more transcripts levels were changed in adult than in seedling tissues. Because shielded tissues including roots, immature ears, and leaves covered with a plastic that absorbs UV-B exhibited altered transcriptome profiles after plant exposure to UV-B, some signal(s) must be transmitted from irradiated to shielded tissues. The time course of transcript abundance changes monitored by RNA blot hybridization and real time RT-PCR indicated that the response kinetics to UV-B are very rapid as some transcript levels are altered within 1 h exposure, in both exposed and shielded tissues. As the same total UV-B irradiation dose applied at three intensities elicited different transcript profiles, transcriptome changes do not show reciprocity. Confirming and extending prior microarray hybridization results, supplemental UV-B increased expression of stress response and ribosomal protein genes, while photosynthesis-associated genes were down regulated in adult leaves. |
| 2 |
GSM10328 |
reciprocity-2h high UV-B-2 |
17,296 |
Universidad Nacional de Rosario |
2003-09-18 |
[cDNA Array] ZmDB Array Unigene-1-01-07 (GPL499) |
RNA |
Zea mays
 |
pooled |
Microarray hybridization was used to assess UV-B responses in both directly exposed and shielded tissues of a maize line deficient in anthocyanin accumulation. Among 347 transcripts found to be regulated after 8 h of high UV-B irradiation, 285 were increased significantly in at least one organ but only 80 were down-regulated by UV-B. Although some genes were regulated by UV-B in more than one sample, most UV-B regulated genes were organ-specific. A larger number of transcript changes occurred in directly exposed than in shielded organs, and more transcripts levels were changed in adult than in seedling tissues. Because shielded tissues including roots, immature ears, and leaves covered with a plastic that absorbs UV-B exhibited altered transcriptome profiles after plant exposure to UV-B, some signal(s) must be transmitted from irradiated to shielded tissues. The time course of transcript abundance changes monitored by RNA blot hybridization and real time RT-PCR indicated that the response kinetics to UV-B are very rapid as some transcript levels are altered within 1 h exposure, in both exposed and shielded tissues. As the same total UV-B irradiation dose applied at three intensities elicited different transcript profiles, transcriptome changes do not show reciprocity. Confirming and extending prior microarray hybridization results, supplemental UV-B increased expression of stress response and ribosomal protein genes, while photosynthesis-associated genes were down regulated in adult leaves. |
| 3 |
GSM10330 |
Reciprocity-4h medium UV-B |
17,296 |
Universidad Nacional de Rosario |
2003-09-18 |
[cDNA Array] ZmDB Array Unigene-1-01-07 (GPL499) |
RNA |
Zea mays
 |
pooled |
Microarray hybridization was used to assess UV-B responses in both directly exposed and shielded tissues of a maize line deficient in anthocyanin accumulation. Among 347 transcripts found to be regulated after 8 h of high UV-B irradiation, 285 were increased significantly in at least one organ but only 80 were down-regulated by UV-B. Although some genes were regulated by UV-B in more than one sample, most UV-B regulated genes were organ-specific. A larger number of transcript changes occurred in directly exposed than in shielded organs, and more transcripts levels were changed in adult than in seedling tissues. Because shielded tissues including roots, immature ears, and leaves covered with a plastic that absorbs UV-B exhibited altered transcriptome profiles after plant exposure to UV-B, some signal(s) must be transmitted from irradiated to shielded tissues. The time course of transcript abundance changes monitored by RNA blot hybridization and real time RT-PCR indicated that the response kinetics to UV-B are very rapid as some transcript levels are altered within 1 h exposure, in both exposed and shielded tissues. As the same total UV-B irradiation dose applied at three intensities elicited different transcript profiles, transcriptome changes do not show reciprocity. Confirming and extending prior microarray hybridization results, supplemental UV-B increased expression of stress response and ribosomal protein genes, while photosynthesis-associated genes were down regulated in adult leaves. |
| 4 |
GSM10332 |
Reciprocity-4h medium UV-B-2 |
17,296 |
Universidad Nacional de Rosario |
2003-09-18 |
[cDNA Array] ZmDB Array Unigene-1-01-07 (GPL499) |
RNA |
Zea mays
 |
pooled |
Microarray hybridization was used to assess UV-B responses in both directly exposed and shielded tissues of a maize line deficient in anthocyanin accumulation. Among 347 transcripts found to be regulated after 8 h of high UV-B irradiation, 285 were increased significantly in at least one organ but only 80 were down-regulated by UV-B. Although some genes were regulated by UV-B in more than one sample, most UV-B regulated genes were organ-specific. A larger number of transcript changes occurred in directly exposed than in shielded organs, and more transcripts levels were changed in adult than in seedling tissues. Because shielded tissues including roots, immature ears, and leaves covered with a plastic that absorbs UV-B exhibited altered transcriptome profiles after plant exposure to UV-B, some signal(s) must be transmitted from irradiated to shielded tissues. The time course of transcript abundance changes monitored by RNA blot hybridization and real time RT-PCR indicated that the response kinetics to UV-B are very rapid as some transcript levels are altered within 1 h exposure, in both exposed and shielded tissues. As the same total UV-B irradiation dose applied at three intensities elicited different transcript profiles, transcriptome changes do not show reciprocity. Confirming and extending prior microarray hybridization results, supplemental UV-B increased expression of stress response and ribosomal protein genes, while photosynthesis-associated genes were down regulated in adult leaves. |
| 5 |
GSM10334 |
Reciprocity-8h high UV-B |
17,296 |
Universidad Nacional de Rosario |
2003-09-18 |
[cDNA Array] ZmDB Array Unigene-1-01-07 (GPL499) |
RNA |
Zea mays
 |
pooled |
Microarray hybridization was used to assess UV-B responses in both directly exposed and shielded tissues of a maize line deficient in anthocyanin accumulation. Among 347 transcripts found to be regulated after 8 h of high UV-B irradiation, 285 were increased significantly in at least one organ but only 80 were down-regulated by UV-B. Although some genes were regulated by UV-B in more than one sample, most UV-B regulated genes were organ-specific. A larger number of transcript changes occurred in directly exposed than in shielded organs, and more transcripts levels were changed in adult than in seedling tissues. Because shielded tissues including roots, immature ears, and leaves covered with a plastic that absorbs UV-B exhibited altered transcriptome profiles after plant exposure to UV-B, some signal(s) must be transmitted from irradiated to shielded tissues. The time course of transcript abundance changes monitored by RNA blot hybridization and real time RT-PCR indicated that the response kinetics to UV-B are very rapid as some transcript levels are altered within 1 h exposure, in both exposed and shielded tissues. As the same total UV-B irradiation dose applied at three intensities elicited different transcript profiles, transcriptome changes do not show reciprocity. Confirming and extending prior microarray hybridization results, supplemental UV-B increased expression of stress response and ribosomal protein genes, while photosynthesis-associated genes were down regulated in adult leaves. |
| 6 |
GSM10339 |
Reciprocity-8h low UV-B |
17,296 |
Universidad Nacional de Rosario |
2003-09-18 |
[cDNA Array] ZmDB Array Unigene-1-01-07 (GPL499) |
RNA |
Zea mays
 |
pooled |
Microarray hybridization was used to assess UV-B responses in both directly exposed and shielded tissues of a maize line deficient in anthocyanin accumulation. Among 347 transcripts found to be regulated after 8 h of high UV-B irradiation, 285 were increased significantly in at least one organ but only 80 were down-regulated by UV-B. Although some genes were regulated by UV-B in more than one sample, most UV-B regulated genes were organ-specific. A larger number of transcript changes occurred in directly exposed than in shielded organs, and more transcripts levels were changed in adult than in seedling tissues. Because shielded tissues including roots, immature ears, and leaves covered with a plastic that absorbs UV-B exhibited altered transcriptome profiles after plant exposure to UV-B, some signal(s) must be transmitted from irradiated to shielded tissues. The time course of transcript abundance changes monitored by RNA blot hybridization and real time RT-PCR indicated that the response kinetics to UV-B are very rapid as some transcript levels are altered within 1 h exposure, in both exposed and shielded tissues. As the same total UV-B irradiation dose applied at three intensities elicited different transcript profiles, transcriptome changes do not show reciprocity. Confirming and extending prior microarray hybridization results, supplemental UV-B increased expression of stress response and ribosomal protein genes, while photosynthesis-associated genes were down regulated in adult leaves. |
| 7 |
GSM10338 |
Reciprocity-8h high UV-B-2 |
17,294 |
Universidad Nacional de Rosario |
2003-09-18 |
[cDNA Array] ZmDB Array Unigene-1-01-07 (GPL499) |
RNA |
Zea mays
 |
pooled |
Microarray hybridization was used to assess UV-B responses in both directly exposed and shielded tissues of a maize line deficient in anthocyanin accumulation. Among 347 transcripts found to be regulated after 8 h of high UV-B irradiation, 285 were increased significantly in at least one organ but only 80 were down-regulated by UV-B. Although some genes were regulated by UV-B in more than one sample, most UV-B regulated genes were organ-specific. A larger number of transcript changes occurred in directly exposed than in shielded organs, and more transcripts levels were changed in adult than in seedling tissues. Because shielded tissues including roots, immature ears, and leaves covered with a plastic that absorbs UV-B exhibited altered transcriptome profiles after plant exposure to UV-B, some signal(s) must be transmitted from irradiated to shielded tissues. The time course of transcript abundance changes monitored by RNA blot hybridization and real time RT-PCR indicated that the response kinetics to UV-B are very rapid as some transcript levels are altered within 1 h exposure, in both exposed and shielded tissues. As the same total UV-B irradiation dose applied at three intensities elicited different transcript profiles, transcriptome changes do not show reciprocity. Confirming and extending prior microarray hybridization results, supplemental UV-B increased expression of stress response and ribosomal protein genes, while photosynthesis-associated genes were down regulated in adult leaves. |
| 8 |
GSM10340 |
Reciprocity-8 h low UV-B-2 |
17,294 |
Universidad Nacional de Rosario |
2003-09-18 |
[cDNA Array] ZmDB Array Unigene-1-01-07 (GPL499) |
RNA |
Zea mays
 |
pooled |
Microarray hybridization was used to assess UV-B responses in both directly exposed and shielded tissues of a maize line deficient in anthocyanin accumulation. Among 347 transcripts found to be regulated after 8 h of high UV-B irradiation, 285 were increased significantly in at least one organ but only 80 were down-regulated by UV-B. Although some genes were regulated by UV-B in more than one sample, most UV-B regulated genes were organ-specific. A larger number of transcript changes occurred in directly exposed than in shielded organs, and more transcripts levels were changed in adult than in seedling tissues. Because shielded tissues including roots, immature ears, and leaves covered with a plastic that absorbs UV-B exhibited altered transcriptome profiles after plant exposure to UV-B, some signal(s) must be transmitted from irradiated to shielded tissues. The time course of transcript abundance changes monitored by RNA blot hybridization and real time RT-PCR indicated that the response kinetics to UV-B are very rapid as some transcript levels are altered within 1 h exposure, in both exposed and shielded tissues. As the same total UV-B irradiation dose applied at three intensities elicited different transcript profiles, transcriptome changes do not show reciprocity. Confirming and extending prior microarray hybridization results, supplemental UV-B increased expression of stress response and ribosomal protein genes, while photosynthesis-associated genes were down regulated in adult leaves. |
| 9 |
GSM10305 |
seedling leaf-8h UV-B |
16,928 |
Universidad Nacional de Rosario |
2003-09-18 |
[cDNA Array] ZmDB Array Unigene-1-01-01 (GPL498) |
RNA |
Zea mays
 |
leaf |
UV-B exposed leaves control leaves (-)UV-B |
| 10 |
GSM10306 |
adult leaf-8h UV-B |
16,928 |
Universidad Nacional de Rosario |
2003-09-18 |
[cDNA Array] ZmDB Array Unigene-1-01-01 (GPL498) |
RNA |
Zea mays
 |
leaf |
UV-B exposed leaves control leaves |
| 11 |
GSM10307 |
adult leaf-UV-B exposed leves covered withCA and PE bag |
16,928 |
Universidad Nacional de Rosario |
2003-09-18 |
[cDNA Array] ZmDB Array Unigene-1-01-01 (GPL498) |
RNA |
Zea mays
 |
leaf |
leaf covered with CA bag leaf covered with PE bag |
| 12 |
GSM10310 |
adult leaf-CA bag vs PE bag in UV-B exposed leaves |
16,928 |
Universidad Nacional de Rosario |
2003-09-18 |
[cDNA Array] ZmDB Array Unigene-1-01-01 (GPL498) |
RNA |
Zea mays
 |
leaf |
adult leaf-CA bag vs PE bag in UV-B exposed leaves |
| 13 |
GSM10313 |
adult leaf-8h UV-B-2 |
16,928 |
Universidad Nacional de Rosario |
2003-09-18 |
[cDNA Array] ZmDB Array Unigene-1-01-01 (GPL498) |
RNA |
Zea mays
 |
leaf |
control leaf UV-B leaf |
| 14 |
GSM10315 |
roots-8h UV-B |
16,928 |
Universidad Nacional de Rosario |
2003-09-18 |
[cDNA Array] ZmDB Array Unigene-1-01-01 (GPL498) |
RNA |
Zea mays
 |
root |
roots-8h UV-B |
| 15 |
GSM10318 |
adult leaf-PE covered leaf in UV-B exposed plants vs PE covered leaf in control plants |
16,928 |
Universidad Nacional de Rosario |
2003-09-18 |
[cDNA Array] ZmDB Array Unigene-1-01-01 (GPL498) |
RNA |
Zea mays
 |
leaf |
PE covered leaf in UV-B exposed plants PE covered leaf in control plants |
| 16 |
GSM10321 |
immature ear-8H UV-B |
16,928 |
Universidad Nacional de Rosario |
2003-09-18 |
[cDNA Array] ZmDB Array Unigene-1-01-01 (GPL498) |
RNA |
Zea mays
 |
other flower/sexual organ |
immature ear-8H UV-B |
| 17 |
GSM10322 |
tassel-8h UV-B |
16,928 |
Universidad Nacional de Rosario |
2003-09-18 |
[cDNA Array] ZmDB Array Unigene-1-01-01 (GPL498) |
RNA |
Zea mays
 |
other flower/sexual organ |
tassel-8h UV-B |
| 18 |
GSM10323 |
immature ear-8h UV-B exposure |
16,928 |
Universidad Nacional de Rosario |
2003-09-18 |
[cDNA Array] ZmDB Array Unigene-1-01-01 (GPL498) |
RNA |
Zea mays
 |
other flower/sexual organ |
immature ear-8h UV-B exposure |
| 19 |
GSM8787 |
(-)PE b,pl-Cy3-CA b,pl-Cy5 |
16,200 |
Universidad Nacional de Rosario |
2003-08-01 |
[cDNA Array] ZmDB 606-Immature Ear Microarray (2 cm) (GPL372) |
RNA |
Zea mays
 |
leaf |
corn adult leaf b,pl after removing PE plastic 1 day-Cy3 corn adult leaf b,plunder CA plastic -Cy5 |
| 20 |
GSM8788 |
(-)PEB,PL-Cy3-CA b,pl-Cy5 |
16,200 |
Universidad Nacional de Rosario |
2003-08-01 |
[cDNA Array] ZmDB 606-Immature Ear Microarray (2 cm) (GPL372) |
RNA |
Zea mays
 |
leaf |
corn adult leaf B,PL one day after removing PE filter-Cy3 corn adult leaf b,pl under CA filter-Cy5 |