| 41 |
GSM451664 |
CDMS3 |
255,984 |
The Ohio State Uinversity |
2009-09-10 |
[GeneChip] Haloferax volcanii DS2 tiled array (GPL9170) |
RNA |
Haloferax volcanii
 |
unclassified |
source_name:DS70, Completely defined media, succinate/glycerol, stationary title:CDMS3 description:specialized growth conditions for this sample: Cells were grown to an OD550 of 1.1. |
| 42 |
GSM451665 |
CXS1 |
255,984 |
The Ohio State Uinversity |
2009-09-10 |
[GeneChip] Haloferax volcanii DS2 tiled array (GPL9170) |
RNA |
Haloferax volcanii
 |
unclassified |
source_name:DS70, Complex media, stationary title:CXS1 description:specialized growth conditions for this sample: Cells were grown to an OD550 of 4.8. |
| 43 |
GSM451666 |
CXS2 |
255,984 |
The Ohio State Uinversity |
2009-09-10 |
[GeneChip] Haloferax volcanii DS2 tiled array (GPL9170) |
RNA |
Haloferax volcanii
 |
unclassified |
source_name:DS70, Complex media, stationary title:CXS2 description:specialized growth conditions for this sample: Cells were grown to an OD550 of 4.8. |
| 44 |
GSM451667 |
CXS3 |
255,984 |
The Ohio State Uinversity |
2009-09-10 |
[GeneChip] Haloferax volcanii DS2 tiled array (GPL9170) |
RNA |
Haloferax volcanii
 |
unclassified |
source_name:DS70, Complex media, stationary title:CXS3 description:specialized growth conditions for this sample: Cells were grown to an OD550 of 4.8. |
| 45 |
GSM451668 |
CDMH1 |
255,984 |
The Ohio State Uinversity |
2009-09-10 |
[GeneChip] Haloferax volcanii DS2 tiled array (GPL9170) |
RNA |
Haloferax volcanii
 |
unclassified |
source_name:DS70, Completely defined media, succinate/glycerol, exponential, histidine as sole nitrogen source title:CDMH1 description:specialized growth conditions for this sample: 5 mM histidine replaced 10 mM NH4Cl as sole nitrogen source |
| 46 |
GSM451669 |
CDMH2 |
255,984 |
The Ohio State Uinversity |
2009-09-10 |
[GeneChip] Haloferax volcanii DS2 tiled array (GPL9170) |
RNA |
Haloferax volcanii
 |
unclassified |
source_name:DS70, Completely defined media, succinate/glycerol, exponential, histidine as sole nitrogen source title:CDMH2 description:specialized growth conditions for this sample: 5 mM histidine replaced 10 mM NH4Cl as sole nitrogen source |
| 47 |
GSM451670 |
CDMH3 |
255,984 |
The Ohio State Uinversity |
2009-09-10 |
[GeneChip] Haloferax volcanii DS2 tiled array (GPL9170) |
RNA |
Haloferax volcanii
 |
unclassified |
source_name:DS70, Completely defined media, succinate/glycerol, exponential, histidine as sole nitrogen source title:CDMH3 description:specialized growth conditions for this sample: 5 mM histidine replaced 10 mM NH4Cl as sole nitrogen source |
| 48 |
GSM451671 |
CDM_N1 |
255,984 |
The Ohio State Uinversity |
2009-09-10 |
[GeneChip] Haloferax volcanii DS2 tiled array (GPL9170) |
RNA |
Haloferax volcanii
 |
unclassified |
source_name:DS70, Completely defined media, succinate/glycerol, exponential, nitrogen starvation title:CDM_N1 description:specialized growth conditions for this sample: A starter culture of H. volcanii DS70 was used as the inoculum (1:1000 dilution) in eight 300 ml cultures of CDM in baffled flasks with foam stoppers (42‹C; 220 rpm). The cultures were grown to an OD550 of 0.1 and the cells were pelleted in sterile centrifuge tubes by centrifugation at 5,000 rpm for 10 minutes at 4‹C in the Beckham JLA10.500 rotor. The cell pellets were washed with basal salts and pelleted by centrifugation as stated above. The supernatant was removed and four of the cell pellets were resuspended in 300 ml of CDM supplemented with succinate and glycerol as carbon sources and 10 mM ammonium chloride (CDM-NR). The other four cell pellets were individually resuspended in 300 ml of CDM supplemented with succinate and glycerol as carbon sources however, no nitrogen source was added (CDM-N). Growth was monitored by the turbidity at 550 nm. Once the four CDM-NR had doubled in turbidity, the culture was allowed to grow for an additional hour. The CDM-N cultures were incubated an equivalent period of time as the CDM-NR cultures. This corresponded to an incubation period of 3.5 hours. RNA was prepared from each and three biological replicates (CDM_N and CDM_NR) were used in array analysis. |
| 49 |
GSM451672 |
CDM_N2 |
255,984 |
The Ohio State Uinversity |
2009-09-10 |
[GeneChip] Haloferax volcanii DS2 tiled array (GPL9170) |
RNA |
Haloferax volcanii
 |
unclassified |
source_name:DS70, Completely defined media, succinate/glycerol, exponential, nitrogen starvation title:CDM_N2 description:specialized growth conditions for this sample: A starter culture of H. volcanii DS70 was used as the inoculum (1:1000 dilution) in eight 300 ml cultures of CDM in baffled flasks with foam stoppers (42‹C; 220 rpm). The cultures were grown to an OD550 of 0.1 and the cells were pelleted in sterile centrifuge tubes by centrifugation at 5,000 rpm for 10 minutes at 4‹C in the Beckham JLA10.500 rotor. The cell pellets were washed with basal salts and pelleted by centrifugation as stated above. The supernatant was removed and four of the cell pellets were resuspended in 300 ml of CDM supplemented with succinate and glycerol as carbon sources and 10 mM ammonium chloride (CDM-NR). The other four cell pellets were individually resuspended in 300 ml of CDM supplemented with succinate and glycerol as carbon sources however, no nitrogen source was added (CDM-N). Growth was monitored by the turbidity at 550 nm. Once the four CDM-NR had doubled in turbidity, the culture was allowed to grow for an additional hour. The CDM-N cultures were incubated an equivalent period of time as the CDM-NR cultures. This corresponded to an incubation period of 3.5 hours. RNA was prepared from each and three biological replicates (CDM_N and CDM_NR) were used in array analysis. |
| 50 |
GSM451673 |
CDM_N3 |
255,984 |
The Ohio State Uinversity |
2009-09-10 |
[GeneChip] Haloferax volcanii DS2 tiled array (GPL9170) |
RNA |
Haloferax volcanii
 |
unclassified |
source_name:DS70, Completely defined media, succinate/glycerol, exponential, nitrogen starvation title:CDM_N3 description:specialized growth conditions for this sample: A starter culture of H. volcanii DS70 was used as the inoculum (1:1000 dilution) in eight 300 ml cultures of CDM in baffled flasks with foam stoppers (42‹C; 220 rpm). The cultures were grown to an OD550 of 0.1 and the cells were pelleted in sterile centrifuge tubes by centrifugation at 5,000 rpm for 10 minutes at 4‹C in the Beckham JLA10.500 rotor. The cell pellets were washed with basal salts and pelleted by centrifugation as stated above. The supernatant was removed and four of the cell pellets were resuspended in 300 ml of CDM supplemented with succinate and glycerol as carbon sources and 10 mM ammonium chloride (CDM-NR). The other four cell pellets were individually resuspended in 300 ml of CDM supplemented with succinate and glycerol as carbon sources however, no nitrogen source was added (CDM-N). Growth was monitored by the turbidity at 550 nm. Once the four CDM-NR had doubled in turbidity, the culture was allowed to grow for an additional hour. The CDM-N cultures were incubated an equivalent period of time as the CDM-NR cultures. This corresponded to an incubation period of 3.5 hours. RNA was prepared from each and three biological replicates (CDM_N and CDM_NR) were used in array analysis. |
| 51 |
GSM451674 |
CDM_NR1 |
255,984 |
The Ohio State Uinversity |
2009-09-10 |
[GeneChip] Haloferax volcanii DS2 tiled array (GPL9170) |
RNA |
Haloferax volcanii
 |
unclassified |
source_name:DS70, Completely defined media, succinate/glycerol, exponential, control recovery for nitrogen starvation title:CDM_NR1 description:specialized growth conditions for this sample: A starter culture of H. volcanii DS70 was used as the inoculum (1:1000 dilution) in eight 300 ml cultures of CDM in baffled flasks with foam stoppers (42‹C; 220 rpm). The cultures were grown to an OD550 of 0.1 and the cells were pelleted in sterile centrifuge tubes by centrifugation at 5,000 rpm for 10 minutes at 4‹C in the Beckham JLA10.500 rotor. The cell pellets were washed with basal salts and pelleted by centrifugation as stated above. The supernatant was removed and four of the cell pellets were resuspended in 300 ml of CDM supplemented with succinate and glycerol as carbon sources and 10 mM ammonium chloride (CDM-NR). The other four cell pellets were individually resuspended in 300 ml of CDM supplemented with succinate and glycerol as carbon sources however, no nitrogen source was added (CDM-N). Growth was monitored by the turbidity at 550 nm. Once the four CDM-NR had doubled in turbidity, the culture was allowed to grow for an additional hour. The CDM-N cultures were incubated an equivalent period of time as the CDM-NR cultures. This corresponded to an incubation period of 3.5 hours. RNA was prepared from each and three biological replicates (CDM_N and CDM_NR) were used in array analysis. |
| 52 |
GSM451675 |
CDM_NR2 |
255,984 |
The Ohio State Uinversity |
2009-09-10 |
[GeneChip] Haloferax volcanii DS2 tiled array (GPL9170) |
RNA |
Haloferax volcanii
 |
unclassified |
source_name:DS70, Completely defined media, succinate/glycerol, exponential, control recovery for nitrogen starvation title:CDM_NR2 description:specialized growth conditions for this sample: A starter culture of H. volcanii DS70 was used as the inoculum (1:1000 dilution) in eight 300 ml cultures of CDM in baffled flasks with foam stoppers (42‹C; 220 rpm). The cultures were grown to an OD550 of 0.1 and the cells were pelleted in sterile centrifuge tubes by centrifugation at 5,000 rpm for 10 minutes at 4‹C in the Beckham JLA10.500 rotor. The cell pellets were washed with basal salts and pelleted by centrifugation as stated above. The supernatant was removed and four of the cell pellets were resuspended in 300 ml of CDM supplemented with succinate and glycerol as carbon sources and 10 mM ammonium chloride (CDM-NR). The other four cell pellets were individually resuspended in 300 ml of CDM supplemented with succinate and glycerol as carbon sources however, no nitrogen source was added (CDM-N). Growth was monitored by the turbidity at 550 nm. Once the four CDM-NR had doubled in turbidity, the culture was allowed to grow for an additional hour. The CDM-N cultures were incubated an equivalent period of time as the CDM-NR cultures. This corresponded to an incubation period of 3.5 hours. RNA was prepared from each and three biological replicates (CDM_N and CDM_NR) were used in array analysis. |
| 53 |
GSM451676 |
CDM_NR3 |
255,984 |
The Ohio State Uinversity |
2009-09-10 |
[GeneChip] Haloferax volcanii DS2 tiled array (GPL9170) |
RNA |
Haloferax volcanii
 |
unclassified |
source_name:DS70, Completely defined media, succinate/glycerol, exponential, control recovery for nitrogen starvation title:CDM_NR3 description:specialized growth conditions for this sample: A starter culture of H. volcanii DS70 was used as the inoculum (1:1000 dilution) in eight 300 ml cultures of CDM in baffled flasks with foam stoppers (42‹C; 220 rpm). The cultures were grown to an OD550 of 0.1 and the cells were pelleted in sterile centrifuge tubes by centrifugation at 5,000 rpm for 10 minutes at 4‹C in the Beckham JLA10.500 rotor. The cell pellets were washed with basal salts and pelleted by centrifugation as stated above. The supernatant was removed and four of the cell pellets were resuspended in 300 ml of CDM supplemented with succinate and glycerol as carbon sources and 10 mM ammonium chloride (CDM-NR). The other four cell pellets were individually resuspended in 300 ml of CDM supplemented with succinate and glycerol as carbon sources however, no nitrogen source was added (CDM-N). Growth was monitored by the turbidity at 550 nm. Once the four CDM-NR had doubled in turbidity, the culture was allowed to grow for an additional hour. The CDM-N cultures were incubated an equivalent period of time as the CDM-NR cultures. This corresponded to an incubation period of 3.5 hours. RNA was prepared from each and three biological replicates (CDM_N and CDM_NR) were used in array analysis. |
| 54 |
GSM451677 |
CDM_C1 |
255,984 |
The Ohio State Uinversity |
2009-09-10 |
[GeneChip] Haloferax volcanii DS2 tiled array (GPL9170) |
RNA |
Haloferax volcanii
 |
unclassified |
source_name:DS70, Completely defined media, succinate/glycerol, exponential, carbon starvation title:CDM_C1 description:specialized growth conditions for this sample: A starter culture of H. volcanii DS70 was used as the inoculum (1:1000 dilution) in eight 300 ml cultures of CDM in baffled flasks with foam stoppers (42‹C; 220 rpm). The cultures were grown to an OD550 of 0.1 and the cells were pelleted in sterile centrifuge tubes by centrifugation at 5,000 rpm for 10 minutes at 4‹C in the Beckham JLA10.500 rotor. The cell pellets were washed with basal salts and pelleted by centrifugation as stated above. The supernatant was removed and four of the cell pellets were resuspended in 300 ml of CDM supplemented with succinate and glycerol as carbon sources (CDM_CR). The other four cell pellets were individually resuspended in 300 ml of CDM; however, no carbon source was added (CDM_C). Growth was monitored by the turbidity at 550 nm. Once the four CDM_CR had doubled in turbidity, the culture was allowed to grow for an additional hour. The CDM_C cultures were incubated an equivalent period of time as the CDM_CR cultures. This corresponded to an incubation period of 3.5 hours. RNA was prepared from each and three biological replicates (CDM_C and CDM_CR) were used in array analysis. |
| 55 |
GSM451678 |
CDM_C2 |
255,984 |
The Ohio State Uinversity |
2009-09-10 |
[GeneChip] Haloferax volcanii DS2 tiled array (GPL9170) |
RNA |
Haloferax volcanii
 |
unclassified |
source_name:DS70, Completely defined media, succinate/glycerol, exponential, carbon starvation title:CDM_C2 description:specialized growth conditions for this sample: A starter culture of H. volcanii DS70 was used as the inoculum (1:1000 dilution) in eight 300 ml cultures of CDM in baffled flasks with foam stoppers (42‹C; 220 rpm). The cultures were grown to an OD550 of 0.1 and the cells were pelleted in sterile centrifuge tubes by centrifugation at 5,000 rpm for 10 minutes at 4‹C in the Beckham JLA10.500 rotor. The cell pellets were washed with basal salts and pelleted by centrifugation as stated above. The supernatant was removed and four of the cell pellets were resuspended in 300 ml of CDM supplemented with succinate and glycerol as carbon sources (CDM_CR). The other four cell pellets were individually resuspended in 300 ml of CDM; however, no carbon source was added (CDM_C). Growth was monitored by the turbidity at 550 nm. Once the four CDM_CR had doubled in turbidity, the culture was allowed to grow for an additional hour. The CDM_C cultures were incubated an equivalent period of time as the CDM_CR cultures. This corresponded to an incubation period of 3.5 hours. RNA was prepared from each and three biological replicates (CDM_C and CDM_CR) were used in array analysis. |
| 56 |
GSM451679 |
CDM_C3 |
255,984 |
The Ohio State Uinversity |
2009-09-10 |
[GeneChip] Haloferax volcanii DS2 tiled array (GPL9170) |
RNA |
Haloferax volcanii
 |
unclassified |
source_name:DS70, Completely defined media, succinate/glycerol, exponential, carbon starvation title:CDM_C3 description:specialized growth conditions for this sample: A starter culture of H. volcanii DS70 was used as the inoculum (1:1000 dilution) in eight 300 ml cultures of CDM in baffled flasks with foam stoppers (42‹C; 220 rpm). The cultures were grown to an OD550 of 0.1 and the cells were pelleted in sterile centrifuge tubes by centrifugation at 5,000 rpm for 10 minutes at 4‹C in the Beckham JLA10.500 rotor. The cell pellets were washed with basal salts and pelleted by centrifugation as stated above. The supernatant was removed and four of the cell pellets were resuspended in 300 ml of CDM supplemented with succinate and glycerol as carbon sources (CDM_CR). The other four cell pellets were individually resuspended in 300 ml of CDM; however, no carbon source was added (CDM_C). Growth was monitored by the turbidity at 550 nm. Once the four CDM_CR had doubled in turbidity, the culture was allowed to grow for an additional hour. The CDM_C cultures were incubated an equivalent period of time as the CDM_CR cultures. This corresponded to an incubation period of 3.5 hours. RNA was prepared from each and three biological replicates (CDM_C and CDM_CR) were used in array analysis. |
| 57 |
GSM451680 |
CDM_CR1 |
255,984 |
The Ohio State Uinversity |
2009-09-10 |
[GeneChip] Haloferax volcanii DS2 tiled array (GPL9170) |
RNA |
Haloferax volcanii
 |
unclassified |
source_name:DS70, Completely defined media, succinate/glycerol, exponential, control recovery for carbon starvation title:CDM_CR1 description:specialized growth conditions for this sample: A starter culture of H. volcanii DS70 was used as the inoculum (1:1000 dilution) in eight 300 ml cultures of CDM in baffled flasks with foam stoppers (42‹C; 220 rpm). The cultures were grown to an OD550 of 0.1 and the cells were pelleted in sterile centrifuge tubes by centrifugation at 5,000 rpm for 10 minutes at 4‹C in the Beckham JLA10.500 rotor. The cell pellets were washed with basal salts and pelleted by centrifugation as stated above. The supernatant was removed and four of the cell pellets were resuspended in 300 ml of CDM supplemented with succinate and glycerol as carbon sources (CDM_CR). The other four cell pellets were individually resuspended in 300 ml of CDM; however, no carbon source was added (CDM_C). Growth was monitored by the turbidity at 550 nm. Once the four CDM_CR had doubled in turbidity, the culture was allowed to grow for an additional hour. The CDM_C cultures were incubated an equivalent period of time as the CDM_CR cultures. This corresponded to an incubation period of 3.5 hours. RNA was prepared from each and three biological replicates (CDM_C and CDM_CR) were used in array analysis. |
| 58 |
GSM451681 |
CDM_CR2 |
255,984 |
The Ohio State Uinversity |
2009-09-10 |
[GeneChip] Haloferax volcanii DS2 tiled array (GPL9170) |
RNA |
Haloferax volcanii
 |
unclassified |
source_name:DS70, Completely defined media, succinate/glycerol, exponential, control recovery for carbon starvation title:CDM_CR2 description:specialized growth conditions for this sample: A starter culture of H. volcanii DS70 was used as the inoculum (1:1000 dilution) in eight 300 ml cultures of CDM in baffled flasks with foam stoppers (42‹C; 220 rpm). The cultures were grown to an OD550 of 0.1 and the cells were pelleted in sterile centrifuge tubes by centrifugation at 5,000 rpm for 10 minutes at 4‹C in the Beckham JLA10.500 rotor. The cell pellets were washed with basal salts and pelleted by centrifugation as stated above. The supernatant was removed and four of the cell pellets were resuspended in 300 ml of CDM supplemented with succinate and glycerol as carbon sources (CDM_CR). The other four cell pellets were individually resuspended in 300 ml of CDM; however, no carbon source was added (CDM_C). Growth was monitored by the turbidity at 550 nm. Once the four CDM_CR had doubled in turbidity, the culture was allowed to grow for an additional hour. The CDM_C cultures were incubated an equivalent period of time as the CDM_CR cultures. This corresponded to an incubation period of 3.5 hours. RNA was prepared from each and three biological replicates (CDM_C and CDM_CR) were used in array analysis. |
| 59 |
GSM451682 |
CDM_CR3 |
255,984 |
The Ohio State Uinversity |
2009-09-10 |
[GeneChip] Haloferax volcanii DS2 tiled array (GPL9170) |
RNA |
Haloferax volcanii
 |
unclassified |
source_name:DS70, Completely defined media, succinate/glycerol, exponential, control recovery for carbon starvation title:CDM_CR3 description:specialized growth conditions for this sample: A starter culture of H. volcanii DS70 was used as the inoculum (1:1000 dilution) in eight 300 ml cultures of CDM in baffled flasks with foam stoppers (42‹C; 220 rpm). The cultures were grown to an OD550 of 0.1 and the cells were pelleted in sterile centrifuge tubes by centrifugation at 5,000 rpm for 10 minutes at 4‹C in the Beckham JLA10.500 rotor. The cell pellets were washed with basal salts and pelleted by centrifugation as stated above. The supernatant was removed and four of the cell pellets were resuspended in 300 ml of CDM supplemented with succinate and glycerol as carbon sources (CDM_CR). The other four cell pellets were individually resuspended in 300 ml of CDM; however, no carbon source was added (CDM_C). Growth was monitored by the turbidity at 550 nm. Once the four CDM_CR had doubled in turbidity, the culture was allowed to grow for an additional hour. The CDM_C cultures were incubated an equivalent period of time as the CDM_CR cultures. This corresponded to an incubation period of 3.5 hours. RNA was prepared from each and three biological replicates (CDM_C and CDM_CR) were used in array analysis. |
| 60 |
GSM451683 |
CDM_sRNAP_E |
255,984 |
The Ohio State Uinversity |
2009-09-10 |
[GeneChip] Haloferax volcanii DS2 tiled array (GPL9170) |
RNA |
Haloferax volcanii
 |
unclassified |
source_name:DS70, small RNA, completely defined media, succinate/glycerol, exponential (pooled) title:CDM_sRNAP_E description:specialized growth conditions for this sample: none |