| 61 |
GSM37453 |
CD1 strain intestine |
21,882 |
University of Toronto |
2004-12-09 |
[Oligo Array] Agilent Custom Mouse Alternative Splicing Oligo Microarray (GPL1738) |
RNA |
Mus musculus
 |
intestine |
Pooled mouse CD1 strain intestine |
| 62 |
GSM37454 |
CD1 strain kidney |
21,882 |
University of Toronto |
2004-12-09 |
[Oligo Array] Agilent Custom Mouse Alternative Splicing Oligo Microarray (GPL1738) |
RNA |
Mus musculus
|
kidney |
Pooled mouse CD1 strain kidney |
| 63 |
GSM37455 |
CD1 strain liver |
21,882 |
University of Toronto |
2004-12-09 |
[Oligo Array] Agilent Custom Mouse Alternative Splicing Oligo Microarray (GPL1738) |
RNA |
Mus musculus
|
liver/hepato |
Pooled mouse CD1 strain liver |
| 64 |
GSM37456 |
CD1 strain lung |
21,882 |
University of Toronto |
2004-12-09 |
[Oligo Array] Agilent Custom Mouse Alternative Splicing Oligo Microarray (GPL1738) |
RNA |
Mus musculus
|
lung |
Pooled mouse CD1 strain lung |
| 65 |
GSM37457 |
CD1 strain muscle |
21,882 |
University of Toronto |
2004-12-09 |
[Oligo Array] Agilent Custom Mouse Alternative Splicing Oligo Microarray (GPL1738) |
RNA |
Mus musculus
|
muscle |
Pooled mouse CD1 strain muscle |
| 66 |
GSM37458 |
CD1 strain salivary |
21,882 |
University of Toronto |
2004-12-09 |
[Oligo Array] Agilent Custom Mouse Alternative Splicing Oligo Microarray (GPL1738) |
RNA |
Mus musculus
|
salivary |
Pooled mouse CD1 strain salivary |
| 67 |
GSM37459 |
CD1 strain spleen |
21,882 |
University of Toronto |
2004-12-09 |
[Oligo Array] Agilent Custom Mouse Alternative Splicing Oligo Microarray (GPL1738) |
RNA |
Mus musculus
|
spleen |
Pooled mouse CD1 strain spleen |
| 68 |
GSM37460 |
CD1 strain testis |
21,882 |
University of Toronto |
2004-12-09 |
[Oligo Array] Agilent Custom Mouse Alternative Splicing Oligo Microarray (GPL1738) |
RNA |
Mus musculus
|
testis |
Pooled mouse CD1 strain testis |
| 69 |
GSM38112 |
SAGE of skeletal muscle of intact mice |
18,383 |
Laval University Hospital Center Research Center |
2004-12-23 |
[SAGE NlaIII] SAGE:10:NlaIII:Mus musculus (GPL11) |
SAGE |
Mus musculus
|
muscle |
skeletal muscle (gastrocnemius) |
| 70 |
GSM38111 |
skeletal muscle of adrenalectomized mice 24h after an injection of glucocorticoids |
12,488 |
Laval University Hospital Center Research Center |
2004-12-23 |
[GeneChip] [MG_U74Av2] Affymetrix Murine Genome U74A Version 2 Array (GPL81) |
RNA |
Mus musculus
|
muscle |
skeletal muscle (gastrocnemius) |
| 71 |
GSM38209 |
skeletal muscle of intact mice |
12,488 |
Laval University Hospital Center Research Center |
2004-12-27 |
[GeneChip] [MG_U74Av2] Affymetrix Murine Genome U74A Version 2 Array (GPL81) |
RNA |
Mus musculus
|
muscle |
skeletal muscle (gastrocnemius) |
| 72 |
GSM38210 |
skeletal muscle of adrenalectomized mice |
12,488 |
Laval University Hospital Center Research Center |
2004-12-27 |
[GeneChip] [MG_U74Av2] Affymetrix Murine Genome U74A Version 2 Array (GPL81) |
RNA |
Mus musculus
|
muscle |
skeletal muscle (gastrocnemius) |
| 73 |
GSM38211 |
skeletal muscle of adrenalectomized mice 3h after an injection of glucocorticoids |
12,468 |
Laval University Hospital Center Research Center |
2004-12-27 |
[GeneChip] [MG_U74Av2] Affymetrix Murine Genome U74A Version 2 Array (GPL81) |
RNA |
Mus musculus
|
muscle |
skeletal muscle (gastrocnemius) |
| 74 |
GSM31975 |
ME7-21dpi-1 |
11,136 |
Canadian Science Centre for Human and Animal Health |
2004-10-10 |
[cDNA Array] BMAP_HGPD (GPL1487) |
RNA |
Mus musculus
|
pooled |
C57BL/6 mice were inoculated intracerebrally with 20 µl of 1% brain homogenate which was obtained from the TSE Resource Centre, Institute for Animal Health, Compton, UK. Mock-infected control mice were inoculated intra-cerebrally with 20 µl of PBS. The brains were removed at the appropriate time-points and stored at -80 C in RNALater (Ambion) until all experimental time points were completed. Total RNA was isolated from the stored tissue samples by homogenization in Trizol reagent (Invitrogen). RNA was further purified using RNeasy columns (Qiagen) according to the manufacturers instructions. 10 µg of total RNA as a template for oligo -dT primed reverse transcription and incorporated amino allyl -dUTP (Sigma) into the cDNA, essentially following the Institute for Genomic Research, Rockville, MD, USA (TIGR) published protocol http://pga.tigr.org/sop/M004_1a.pdf The remaining RNA template was hydrolysed and the cDNA product purified using the QIAquick PCR purification system (Qiagen). cDNA was dried down, then resuspended in 0.1 M Na2CO3, pH9.0 buffer, and incubated for 1 hour in either an Alexa Fluor555 (AF555) or Alexa Fluor647(AF647) mono-functional reactive dye (Molecular Probes) made up in DMSO. Labelled cDNA was also purified using QIAquick PCR purification columns. Labelled cDNA was dried down and resuspended in 60 µl DIG Easy Hyb hybridisation buffer (Roche) containing calf thymus DNA (Sigma) and yeast tRNA (Life Technologies). The labelled cDNAs were applied to arrays under M Series Lifter Slips (Erie Scientific Company) and the slides incubated in a hybridisation chamber at 42oC for 16 hours. Following hybridisation lifter slips were removed and the arrays washed a total of 3 times for 5 minutes first in1 X SSC, 0.2% SDS, and then 3 times for 5 minutes in 0.1 X SSC, 0.2% SDS pre-warmed to 42°C. A final wash in 0.1 X SSC at room temperature was performed, and slides centrifuged to dry. Slides were immediately scanned using a VersArray scanner (BioRad) at appropriate laser power and PhotoMultiplier Tube settings so that high intensity spots were not saturated. Spots were quantitated and background signals removed using ArrayPro software (Media Cybernetics, Inc). |
| 75 |
GSM31976 |
ME7-21dpi-2 |
11,136 |
Canadian Science Centre for Human and Animal Health |
2004-10-10 |
[cDNA Array] BMAP_HGPD (GPL1487) |
RNA |
Mus musculus
|
pooled |
C57BL/6 mice were inoculated intracerebrally with 20 µl of 1% brain homogenate which was obtained from the TSE Resource Centre, Institute for Animal Health, Compton, UK. Mock-infected control mice were inoculated intra-cerebrally with 20 µl of PBS. The brains were removed at the appropriate time-points and stored at -80 C in RNALater (Ambion) until all experimental time points were completed. Total RNA was isolated from the stored tissue samples by homogenization in Trizol reagent (Invitrogen). RNA was further purified using RNeasy columns (Qiagen) according to the manufacturers instructions. 10 µg of total RNA as a template for oligo -dT primed reverse transcription and incorporated amino allyl -dUTP (Sigma) into the cDNA, essentially following the Institute for Genomic Research, Rockville, MD, USA (TIGR) published protocol http://pga.tigr.org/sop/M004_1a.pdf The remaining RNA template was hydrolysed and the cDNA product purified using the QIAquick PCR purification system (Qiagen). cDNA was dried down, then resuspended in 0.1 M Na2CO3, pH9.0 buffer, and incubated for 1 hour in either an Alexa Fluor555 (AF555) or Alexa Fluor647(AF647) mono-functional reactive dye (Molecular Probes) made up in DMSO. Labelled cDNA was also purified using QIAquick PCR purification columns. Labelled cDNA was dried down and resuspended in 60 µl DIG Easy Hyb hybridisation buffer (Roche) containing calf thymus DNA (Sigma) and yeast tRNA (Life Technologies). The labelled cDNAs were applied to arrays under M Series Lifter Slips (Erie Scientific Company) and the slides incubated in a hybridisation chamber at 42oC for 16 hours. Following hybridisation lifter slips were removed and the arrays washed a total of 3 times for 5 minutes first in1 X SSC, 0.2% SDS, and then 3 times for 5 minutes in 0.1 X SSC, 0.2% SDS pre-warmed to 42°C. A final wash in 0.1 X SSC at room temperature was performed, and slides centrifuged to dry. Slides were immediately scanned using a VersArray scanner (BioRad) at appropriate laser power and PhotoMultiplier Tube settings so that high intensity spots were not saturated. Spots were quantitated and background signals removed using ArrayPro software (Media Cybernetics, Inc). |
| 76 |
GSM31977 |
ME7-21dpi-3 |
11,136 |
Canadian Science Centre for Human and Animal Health |
2004-10-10 |
[cDNA Array] BMAP_HGPD (GPL1487) |
RNA |
Mus musculus
|
pooled |
C57BL/6 mice were inoculated intracerebrally with 20 µl of 1% brain homogenate which was obtained from the TSE Resource Centre, Institute for Animal Health, Compton, UK. Mock-infected control mice were inoculated intra-cerebrally with 20 µl of PBS. The brains were removed at the appropriate time-points and stored at -80 C in RNALater (Ambion) until all experimental time points were completed. Total RNA was isolated from the stored tissue samples by homogenization in Trizol reagent (Invitrogen). RNA was further purified using RNeasy columns (Qiagen) according to the manufacturers instructions. 10 µg of total RNA as a template for oligo -dT primed reverse transcription and incorporated amino allyl -dUTP (Sigma) into the cDNA, essentially following the Institute for Genomic Research, Rockville, MD, USA (TIGR) published protocol http://pga.tigr.org/sop/M004_1a.pdf The remaining RNA template was hydrolysed and the cDNA product purified using the QIAquick PCR purification system (Qiagen). cDNA was dried down, then resuspended in 0.1 M Na2CO3, pH9.0 buffer, and incubated for 1 hour in either an Alexa Fluor555 (AF555) or Alexa Fluor647(AF647) mono-functional reactive dye (Molecular Probes) made up in DMSO. Labelled cDNA was also purified using QIAquick PCR purification columns. Labelled cDNA was dried down and resuspended in 60 µl DIG Easy Hyb hybridisation buffer (Roche) containing calf thymus DNA (Sigma) and yeast tRNA (Life Technologies). The labelled cDNAs were applied to arrays under M Series Lifter Slips (Erie Scientific Company) and the slides incubated in a hybridisation chamber at 42oC for 16 hours. Following hybridisation lifter slips were removed and the arrays washed a total of 3 times for 5 minutes first in1 X SSC, 0.2% SDS, and then 3 times for 5 minutes in 0.1 X SSC, 0.2% SDS pre-warmed to 42°C. A final wash in 0.1 X SSC at room temperature was performed, and slides centrifuged to dry. Slides were immediately scanned using a VersArray scanner (BioRad) at appropriate laser power and PhotoMultiplier Tube settings so that high intensity spots were not saturated. Spots were quantitated and background signals removed using ArrayPro software (Media Cybernetics, Inc). |
| 77 |
GSM31978 |
ME7-21dpi-4 |
11,136 |
Canadian Science Centre for Human and Animal Health |
2004-10-10 |
[cDNA Array] BMAP_HGPD (GPL1487) |
RNA |
Mus musculus
|
pooled |
C57BL/6 mice were inoculated intracerebrally with 20 µl of 1% brain homogenate which was obtained from the TSE Resource Centre, Institute for Animal Health, Compton, UK. Mock-infected control mice were inoculated intra-cerebrally with 20 µl of PBS. The brains were removed at the appropriate time-points and stored at -80 C in RNALater (Ambion) until all experimental time points were completed. Total RNA was isolated from the stored tissue samples by homogenization in Trizol reagent (Invitrogen). RNA was further purified using RNeasy columns (Qiagen) according to the manufacturers instructions. 10 µg of total RNA as a template for oligo -dT primed reverse transcription and incorporated amino allyl -dUTP (Sigma) into the cDNA, essentially following the Institute for Genomic Research, Rockville, MD, USA (TIGR) published protocol http://pga.tigr.org/sop/M004_1a.pdf The remaining RNA template was hydrolysed and the cDNA product purified using the QIAquick PCR purification system (Qiagen). cDNA was dried down, then resuspended in 0.1 M Na2CO3, pH9.0 buffer, and incubated for 1 hour in either an Alexa Fluor555 (AF555) or Alexa Fluor647(AF647) mono-functional reactive dye (Molecular Probes) made up in DMSO. Labelled cDNA was also purified using QIAquick PCR purification columns. Labelled cDNA was dried down and resuspended in 60 µl DIG Easy Hyb hybridisation buffer (Roche) containing calf thymus DNA (Sigma) and yeast tRNA (Life Technologies). The labelled cDNAs were applied to arrays under M Series Lifter Slips (Erie Scientific Company) and the slides incubated in a hybridisation chamber at 42oC for 16 hours. Following hybridisation lifter slips were removed and the arrays washed a total of 3 times for 5 minutes first in1 X SSC, 0.2% SDS, and then 3 times for 5 minutes in 0.1 X SSC, 0.2% SDS pre-warmed to 42°C. A final wash in 0.1 X SSC at room temperature was performed, and slides centrifuged to dry. Slides were immediately scanned using a VersArray scanner (BioRad) at appropriate laser power and PhotoMultiplier Tube settings so that high intensity spots were not saturated. Spots were quantitated and background signals removed using ArrayPro software (Media Cybernetics, Inc). |
| 78 |
GSM31979 |
ME7-21dpi-5 |
11,136 |
Canadian Science Centre for Human and Animal Health |
2004-10-10 |
[cDNA Array] BMAP_HGPD (GPL1487) |
RNA |
Mus musculus
|
pooled |
C57BL/6 mice were inoculated intracerebrally with 20 µl of 1% brain homogenate which was obtained from the TSE Resource Centre, Institute for Animal Health, Compton, UK. Mock-infected control mice were inoculated intra-cerebrally with 20 µl of PBS. The brains were removed at the appropriate time-points and stored at -80 C in RNALater (Ambion) until all experimental time points were completed. Total RNA was isolated from the stored tissue samples by homogenization in Trizol reagent (Invitrogen). RNA was further purified using RNeasy columns (Qiagen) according to the manufacturers instructions. 10 µg of total RNA as a template for oligo -dT primed reverse transcription and incorporated amino allyl -dUTP (Sigma) into the cDNA, essentially following the Institute for Genomic Research, Rockville, MD, USA (TIGR) published protocol http://pga.tigr.org/sop/M004_1a.pdf The remaining RNA template was hydrolysed and the cDNA product purified using the QIAquick PCR purification system (Qiagen). cDNA was dried down, then resuspended in 0.1 M Na2CO3, pH9.0 buffer, and incubated for 1 hour in either an Alexa Fluor555 (AF555) or Alexa Fluor647(AF647) mono-functional reactive dye (Molecular Probes) made up in DMSO. Labelled cDNA was also purified using QIAquick PCR purification columns. Labelled cDNA was dried down and resuspended in 60 µl DIG Easy Hyb hybridisation buffer (Roche) containing calf thymus DNA (Sigma) and yeast tRNA (Life Technologies). The labelled cDNAs were applied to arrays under M Series Lifter Slips (Erie Scientific Company) and the slides incubated in a hybridisation chamber at 42oC for 16 hours. Following hybridisation lifter slips were removed and the arrays washed a total of 3 times for 5 minutes first in1 X SSC, 0.2% SDS, and then 3 times for 5 minutes in 0.1 X SSC, 0.2% SDS pre-warmed to 42°C. A final wash in 0.1 X SSC at room temperature was performed, and slides centrifuged to dry. Slides were immediately scanned using a VersArray scanner (BioRad) at appropriate laser power and PhotoMultiplier Tube settings so that high intensity spots were not saturated. Spots were quantitated and background signals removed using ArrayPro software (Media Cybernetics, Inc). |
| 79 |
GSM31980 |
ME7-21dpi-6 |
11,136 |
Canadian Science Centre for Human and Animal Health |
2004-10-10 |
[cDNA Array] BMAP_HGPD (GPL1487) |
RNA |
Mus musculus
|
pooled |
C57BL/6 mice were inoculated intracerebrally with 20 µl of 1% brain homogenate which was obtained from the TSE Resource Centre, Institute for Animal Health, Compton, UK. Mock-infected control mice were inoculated intra-cerebrally with 20 µl of PBS. The brains were removed at the appropriate time-points and stored at -80 C in RNALater (Ambion) until all experimental time points were completed. Total RNA was isolated from the stored tissue samples by homogenization in Trizol reagent (Invitrogen). RNA was further purified using RNeasy columns (Qiagen) according to the manufacturers instructions. 10 µg of total RNA as a template for oligo -dT primed reverse transcription and incorporated amino allyl -dUTP (Sigma) into the cDNA, essentially following the Institute for Genomic Research, Rockville, MD, USA (TIGR) published protocol http://pga.tigr.org/sop/M004_1a.pdf The remaining RNA template was hydrolysed and the cDNA product purified using the QIAquick PCR purification system (Qiagen). cDNA was dried down, then resuspended in 0.1 M Na2CO3, pH9.0 buffer, and incubated for 1 hour in either an Alexa Fluor555 (AF555) or Alexa Fluor647(AF647) mono-functional reactive dye (Molecular Probes) made up in DMSO. Labelled cDNA was also purified using QIAquick PCR purification columns. Labelled cDNA was dried down and resuspended in 60 µl DIG Easy Hyb hybridisation buffer (Roche) containing calf thymus DNA (Sigma) and yeast tRNA (Life Technologies). The labelled cDNAs were applied to arrays under M Series Lifter Slips (Erie Scientific Company) and the slides incubated in a hybridisation chamber at 42oC for 16 hours. Following hybridisation lifter slips were removed and the arrays washed a total of 3 times for 5 minutes first in1 X SSC, 0.2% SDS, and then 3 times for 5 minutes in 0.1 X SSC, 0.2% SDS pre-warmed to 42°C. A final wash in 0.1 X SSC at room temperature was performed, and slides centrifuged to dry. Slides were immediately scanned using a VersArray scanner (BioRad) at appropriate laser power and PhotoMultiplier Tube settings so that high intensity spots were not saturated. Spots were quantitated and background signals removed using ArrayPro software (Media Cybernetics, Inc). |
| 80 |
GSM31981 |
ME7-21dpi-7 |
11,136 |
Canadian Science Centre for Human and Animal Health |
2004-10-10 |
[cDNA Array] BMAP_HGPD (GPL1487) |
RNA |
Mus musculus
|
pooled |
C57BL/6 mice were inoculated intracerebrally with 20 µl of 1% brain homogenate which was obtained from the TSE Resource Centre, Institute for Animal Health, Compton, UK. Mock-infected control mice were inoculated intra-cerebrally with 20 µl of PBS. The brains were removed at the appropriate time-points and stored at -80 C in RNALater (Ambion) until all experimental time points were completed. Total RNA was isolated from the stored tissue samples by homogenization in Trizol reagent (Invitrogen). RNA was further purified using RNeasy columns (Qiagen) according to the manufacturers instructions. 10 µg of total RNA as a template for oligo -dT primed reverse transcription and incorporated amino allyl -dUTP (Sigma) into the cDNA, essentially following the Institute for Genomic Research, Rockville, MD, USA (TIGR) published protocol http://pga.tigr.org/sop/M004_1a.pdf The remaining RNA template was hydrolysed and the cDNA product purified using the QIAquick PCR purification system (Qiagen). cDNA was dried down, then resuspended in 0.1 M Na2CO3, pH9.0 buffer, and incubated for 1 hour in either an Alexa Fluor555 (AF555) or Alexa Fluor647(AF647) mono-functional reactive dye (Molecular Probes) made up in DMSO. Labelled cDNA was also purified using QIAquick PCR purification columns. Labelled cDNA was dried down and resuspended in 60 µl DIG Easy Hyb hybridisation buffer (Roche) containing calf thymus DNA (Sigma) and yeast tRNA (Life Technologies). The labelled cDNAs were applied to arrays under M Series Lifter Slips (Erie Scientific Company) and the slides incubated in a hybridisation chamber at 42oC for 16 hours. Following hybridisation lifter slips were removed and the arrays washed a total of 3 times for 5 minutes first in1 X SSC, 0.2% SDS, and then 3 times for 5 minutes in 0.1 X SSC, 0.2% SDS pre-warmed to 42°C. A final wash in 0.1 X SSC at room temperature was performed, and slides centrifuged to dry. Slides were immediately scanned using a VersArray scanner (BioRad) at appropriate laser power and PhotoMultiplier Tube settings so that high intensity spots were not saturated. Spots were quantitated and background signals removed using ArrayPro software (Media Cybernetics, Inc). |